Immunotoxicity of nanoparticle nTiO 2 to a commercial marine bivalve species, Tegillarca granosa

Shi, Wei; Han, Yu; Guo, Cheng; Zhao, Xinguo; Liu, Saixi; Su, Wenhao; Zha, Shanjie; Wang, Yichen; Liu, Guangxu

doi:10.1016/j.fsi.2017.05.036

Cited by 61 publications

(25 citation statements)

References 55 publications

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“…Alterations in hemocyte morpho-functional parameters have been reported for bivalves after exposure, both in vitro and in vivo, to organic and inorganic contaminants, such as heavy metals [38][39][40], organotins [37] and polycyclic aromatic hydrocarbons (PAHs) [41,42]. More recently, increasing attention has been addressed to the evaluation of potential immunotoxic effects of emerging contaminants, such as pharmaceuticals and personal care products (PPCPs) [43][44][45][46][47] and nanoparticles [48][49][50].…”

Section: Discussionmentioning

confidence: 99%

Exposure to Decreased pH and Caffeine Affects Hemocyte Parameters in the Mussel Mytilus galloprovincialis

Munari

Matozzo

Benetello

et al. 2020

JMSE

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Combined effects of reduced pH, as predicted under climate change scenarios, and the most popular and widely used stimulant caffeine were assessed in hemocyte parameters of the mussel Mytilus galloprovincialis, being hemocytes involved in immune defense. Bivalves were exposed for one week to natural pH (8.1) and two reduced pH values (pH −0.4 units and pH −0.7 units). Exposure continued for additional two weeks, both in the absence and in the presence of environmentally relevant concentrations of caffeine (0.05 and 0.5 µg/L). Hemocyte parameters (total hemocyte count, hemocyte volume and diameter, neutral red uptake and hemocyte proliferation) were measured after 7 days of exposure to pH only, and after 14 (T1) and 21 (T2) days of exposure to the various pH*caffeine combinations. At all sampling times, pH significantly affected all the biological variables considered, whereas caffeine exhibited a significant influence at T2 only. Among the various hemocyte parameters, caffeine caused a significant increase in total hemocyte count at T2, and in hemocyte volume and diameter at both T1 and T2, when a significant interaction between pH and caffeine was also found. Overall, results demonstrated that hemocyte functionality was strongly influenced by the experimental conditions tested. Further studies are needed to assess combined effects of climate changes and emerging contaminants on bivalve immune system when challenged with environmental pathogens.

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Section: Discussionmentioning

confidence: 99%

Exposure to Decreased pH and Caffeine Affects Hemocyte Parameters in the Mussel Mytilus galloprovincialis

Munari

Matozzo

Benetello

et al. 2020

JMSE

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“…A number of studies have suggested that TiO 2 NPs could pose toxicity to several aquatic organisms including microbes, algae, invertebrates and fish (Chen et al, 2012). In a species of ark clam known as the blood clam Tegillarca granosa, Shi et al (2017) treated the clam with 10 and 100 mg/l TiO 2 NP for 30 days. The authors reported that RBCs were significantly decreased from 79.76% to 70.98% in comparison to the negative control.…”

Section: Discussionmentioning

confidence: 99%

Assessment of titanium dioxide nanoparticle as treatment of Aeromonas hydrophila infection in Oreochromis niloticus

Sherif¹,

Al‐Sokary²,

Esam

2019

J Hellenic Vet Med Soc

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Nanoproducts became widely used materials all over the world. Antimicrobial properties of titanium dioxide (TiO2) nanoparticle (NP) were examined against Aeromonas hydrophila (A. hydrophila) bacteria and the minimum inhibitory concentration (MIC) was found to be 20 μg/ml of TiO2NP. In addition, the treatment efficacy of TiO2NP was examined in Oreochromis niloticus (O. niloticus) infected with A. hydrophila. One hundred and eighty fish (54±2.4 g b.w.) were divided into six groups (G). O. niloticus in G1, G2 and G3 were fed for 30 days with 0, 20 and 100 μg/g b.w. TiO2NP, respectively, while G4, G5 and G6 were i.p. injected with 0.2 ml distal water, 20 and 100 μg/g b.w. TiO2NP, respectively, for three times with ten days of interval. The blood parameters as well as some of the biochemical parameters of O. niloticus that received high dosage of TiO2NP were significantly affected regardless to the administration route. Elevation of the activities of glutathione peroxidase (GPx) and metallothionine (MT) were recorded with the high dosage. Furthermore, O. niloticus subjected to high dosage of TiO2NP had the lower survival rate (SR%) especially with the injection route (50%). On the other hand, no significant changes were demonstrated with the perceived TiO2NP MIC. The mortality rate (MR%) of challenged O. niloticus against A. hydrophila was decreased in case of TiO2NP MIC exposure, as G2 and G5 revealed 20 and 30%, respectively. Therefore, the 20 μg/g b.w. of TiO2NP could safely protect O. niloticus against A. hydrophila infection since no health hazards was observed. Meanwhile, health status of O. niloticus was adversely affected with high dosage of TiO2NP irrespective to the route of administration.

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“…Following the methods described by Evelyne et al (1991) and Shi et al (2017) , after a 2-week experimental treatment, three individuals were randomly picked from each trial for the phagocytosis assays. A yeast (Instant dry yeast, AngelYeast) suspension containing (1.45 ± 0.05) × 10 8 yeast cells per ml was prepared by dissolving 7 mg of yeast powder in 1000 μl of Alsever’s solution (ALS, Noble Ryder) and supersonic vibration was used for a sufficient homogenized mixing.…”

Section: Methodsmentioning

confidence: 99%

“…Nevertheless, recent studies have shown that OA could hamper the immune responses of various marine bivalve species, such as Crassostrea gigas ( Wang et al, 2016 ), Mercenaria mercenaria ( Ivanina et al, 2016 ), and Mytilus edulis ( Mackenzie et al, 2014 ). Among all the parameters evaluating immune responses, the phagocytosis of hemocytes, one of the most important defense measures in non-specific immunity ( Wang et al, 2012 ), including the engulfment and degradation of pathogen particles, has been widely used in previous studies ( Liu et al, 2016 ; Shi et al, 2017 ; Su et al, 2017 ). Though an OA-induced reduction in phagocytosis has been reported in bivalve species, the mechanism manifesting this impact still remains unclear ( Canesi et al, 2002 ; Sokolnikova et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

“…The blood clam occupies an important ecological niche in the intertidal zone and is therefore crucial for the material and energy flows of intertidal ecosystems ( Liu et al, 2014 ). In addition, inhabiting in the intertidal zone where surface runoff converges into the sea, the blood clam is often challenged by various pollutants and deemed as an ideal marine bivalve species with which to investigate the impacts of environmental stresses on immune responses ( Zhu et al, 2011 ; Liu et al, 2016 ; Shi et al, 2017 ; Su et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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Ocean Acidification Affects the Cytoskeleton, Lysozymes, and Nitric Oxide of Hemocytes: A Possible Explanation for the Hampered Phagocytosis in Blood Clams, Tegillarca granosa

Rong

Zha

et al. 2018

Front. Physiol.

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An enormous amount of anthropogenic carbon dioxide (CO2) has been dissolved into the ocean, leading to a lower pH and changes in the chemical properties of seawater, which has been termed ocean acidification (OA). The impacts of pCO2-driven acidification on immunity have been revealed recently in various marine organisms. However, the mechanism causing the reduction in phagocytosis still remains unclear. Therefore, the impacts of pCO2-driven OA at present and near-future levels (pH values of 8.1, 7.8, and 7.4) on the rate of phagocytosis, the abundance of cytoskeleton components, the levels of nitric oxide (NO), and the concentration and activity of lysozymes (LZM) of hemocytes were investigated in a commercial bivalve species, the blood clam (Tegillarca granosa). In addition, the effects of OA on the expression of genes regulating actin skeleton and nitric oxide synthesis 2 (NOS2) were also analyzed. The results obtained showed that the phagocytic rate, cytoskeleton component abundance, concentration and activity of LZM of hemocytes were all significantly reduced after a 2-week exposure to the future OA scenario of a pH of 7.4. On the contrary, a remarkable increase in the concentration of NO compared to that of the control was detected in clams exposed to OA. Furthermore, the expression of genes regulating the actin cytoskeleton and NOS were significantly up-regulated after OA exposure. Though the mechanism causing phagocytosis seemed to be complicated based on the results obtained in the present study and those reported previously, our results suggested that OA may reduce the phagocytosis of hemocytes by (1) decreasing the abundance of cytoskeleton components and therefore hampering the cytoskeleton-mediated process of engulfment, (2) reducing the concentration and activity of LZM and therefore constraining the degradation of the engulfed pathogen through an oxygen-independent pathway, and (3) inducing the production of NO, which may negatively regulate immune responses.

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Immunotoxicity of nanoparticle nTiO 2 to a commercial marine bivalve species, Tegillarca granosa

Cited by 61 publications

References 55 publications

Exposure to Decreased pH and Caffeine Affects Hemocyte Parameters in the Mussel Mytilus galloprovincialis

Exposure to Decreased pH and Caffeine Affects Hemocyte Parameters in the Mussel Mytilus galloprovincialis

Assessment of titanium dioxide nanoparticle as treatment of Aeromonas hydrophila infection in Oreochromis niloticus

Ocean Acidification Affects the Cytoskeleton, Lysozymes, and Nitric Oxide of Hemocytes: A Possible Explanation for the Hampered Phagocytosis in Blood Clams, Tegillarca granosa

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