Impact and Diagnosis of Minimal or Grade 1 Hepatic Encephalopathy

Lauridsen, Mette Munk; Vilstrup, Hendrik

doi:10.1007/978-3-319-76798-7_4

Cited by 2 publications

(1 citation statement)

References 32 publications

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“…Patients with hyperammonemia may develop hepatic encephalopathy, which manifests as a wide spectrum of neurological or psychiatric abnormalities ranging from mild cognitive and behavioral alterations to coma 8 . Even the mildest forms of hepatic encephalopathy negatively impact the quality of life of both patients and caregivers, while overt and recurrent episodes of hepatic encephalopathy are related to poor prognosis, mortality, and increased healthcare burden 9 .…”

Section: Introductionmentioning

confidence: 99%

Optimization of an Ammonia Assay based on Transmembrane pH-gradient Polymersomes

Spyrogianni

Gourmel

Hofmann

et al. 2021

Preprint

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Reliable ammonia quantification assays are essential for monitoring ammonemia in patients with liver diseases. In this study, we describe the development process of a microplate-based assay for accurate, precise, and robust ammonia quantification in biological fluids, following regulatory guidelines on bioanalytical method validation. The assay is based on transmembrane pH-gradient polymersomes that encapsulate a pH-sensitive ratiometric fluorophore, the fluorescence signal of which correlates with the ammonia concentration in the sample. Using four-parameter logistic regression, the assay had a large quantification range (30–800 µM ammonia). As for selectivity, the presence of amino acids or pyruvate (up to clinically relevant concentrations) showed no assay interference. In samples with low bilirubin levels, polymersomes containing the fluorophore pyranine provided accurate ammonia quantification. In samples with high bilirubin concentrations, billirubin’s optical interference was alleviated when replacing pyranine with a close to near-infrared hemicyanine fluorophore. Finally, the assay could correctly retrieve the ammonia concentration in ammonia-spiked human plasma samples, which was confirmed by comparing our measurements with the data obtained using a commercially available point-of-care device for ammonia.

show abstract

Section: Introductionmentioning

confidence: 99%

Optimization of an Ammonia Assay based on Transmembrane pH-gradient Polymersomes

Spyrogianni

Gourmel

Hofmann

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

Optimization of an ammonia assay based on transmembrane pH-gradient polymersomes

Spyrogianni

Gourmel

Hofmann

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Reliable ammonia quantification assays are essential for monitoring ammonemia in patients with liver diseases. In this study, we describe the development process of a microplate-based assay for accurate, precise, and robust ammonia quantification in biological fluids, following regulatory guidelines on bioanalytical method validation. The assay is based on transmembrane pH-gradient polymersomes that encapsulate a pH-sensitive ratiometric fluorophore, the fluorescence signal of which correlates with the ammonia concentration in the sample. Using a four-parameter logistic regression, the assay had a large quantification range (30–800 μM ammonia). As for selectivity, the presence of amino acids or pyruvate (up to clinically relevant concentrations) showed no assay interference. In samples with low bilirubin levels, polymersomes containing the fluorophore pyranine provided accurate ammonia quantification. In samples with high bilirubin concentrations, billirubin’s optical interference was alleviated when replacing pyranine with a close to near-infrared hemicyanine fluorophore. Finally, the assay could correctly retrieve the ammonia concentration in ammonia-spiked human plasma samples, which was confirmed by comparing our measurements with the data obtained using a commercially available point-of-care device for ammonia.

show abstract

Impact and Diagnosis of Minimal or Grade 1 Hepatic Encephalopathy

Cited by 2 publications

References 32 publications

Optimization of an Ammonia Assay based on Transmembrane pH-gradient Polymersomes

Optimization of an Ammonia Assay based on Transmembrane pH-gradient Polymersomes

Optimization of an ammonia assay based on transmembrane pH-gradient polymersomes

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