Impact and interaction of lipophilic antioxidants in mutants and transgenic plants

Woitsch, Sonja; Römer, Susanne

doi:10.1016/j.jplph.2005.04.007

Cited by 4 publications

(2 citation statements)

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“…2c). These data thus demonstrate that the alteration of VTE3(1) mRNA levels directly affects tocopherol content and composition in tomato fruit and furthermore supports earlier hypotheses that fruit redox status is balanced by mechanisms which impact the metabolic pathways associated with both tocopherol and carotenoid metabolism [20][21][22] .…”

Section: Resultssupporting

confidence: 87%

Natural occurring epialleles determine vitamin E accumulation in tomato fruits

et al. 2014

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Vitamin E (VTE) content is a low heritability nutritional trait for which the genetic determinants are poorly understood. Here, we focus on a previously detected major tomato VTE quantitative trait loci (QTL; mQTL 9-2-6 ) and identify the causal gene as one encoding a 2-methyl-6-phytylquinol methyltransferase (namely VTE3 (1)) that catalyses one of the final steps in the biosynthesis of g-and a-tocopherols, which are the main forms of VTE. By reverse genetic approaches, expression analyses, siRNA profiling and DNA methylation assays, we demonstrate that mQTL 9-2-6 is an expression QTL associated with differential methylation of a SINE retrotransposon located in the promoter region of VTE3(1). Promoter DNA methylation can be spontaneously reverted leading to different epialleles affecting VTE3(1) expression and VTE content in fruits. These findings indicate therefore that naturally occurring epialleles are responsible for regulation of a nutritionally important metabolic QTL and provide direct evidence of a role for epigenetics in the determination of agronomic traits.

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Section: Resultssupporting

confidence: 87%

Natural occurring epialleles determine vitamin E accumulation in tomato fruits

et al. 2014

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“…Additionally, tocopherol can be functionally replaced by other antioxidative compounds e.g. ascorbate and glutathione or by increase in xantophyll cycle activity (Fryer 1992;Grabes et al 2001;Kanwischer et al 2005;Woitsch and Römer 2005).…”

Section: Discussionmentioning

confidence: 99%

Tocopherol content and enzymatic antioxidant activities in chloroplasts from NaCl-stressed tomato plants

et al. 2008

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Changes in tocopherol, chlorophyll and TBARS levels and the activities of antioxidant enzymes i.e., GSH-Px, GST, and SOD in chloroplasts of tomato plants subjected to moderate (50 mM) and severe (150 mM) NaCl stress were determined. Increase in tocopherol content around the second day under both stresses did not correlate with the chlorophyll degradation while such correlation was observed from the fifth day of severe stress. The activities of GSH-Px and GST as well as TBARS content showed NaCl-induced enhancement which was dose-and time-dependent. However, chloroplastic SOD was rather not involved in the response of tomato plants to NaCl stress. The obtained results suggest that under the moderate stress similarly as in the early phase of severe stress tocopherol functions as a typical antioxidant, while in the late phase of the latter it may be involved in senescence signaling pathway and enables the recovery and recycling of the compounds significant for a plant organism.

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