Impact of 24‐h Cooling Prior to Freezing on the Survival of Domestic Cat (<i>Felis catus</i>) Epididymal Sperm

Martins, J. P. N; Villaverde, Aisb; Lima, Alfredo Feio da Maia; Steagall, Paulo V.; Ferreira, Jair Camargo; Taconeli, César Augusto; Lopes, Denivaldo

doi:10.1111/j.1439-0531.2009.01432.x

Cited by 9 publications

(8 citation statements)

References 17 publications

(50 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After 10 min, the processed sperm were loaded in 0.25 ml straws (Minitub GmBH, Tiefenbach, Germany) and then frozen. The freezing procedure was undertaken in a styrofoam box by placing the filled straws horizontally 6 cm above the liquid nitrogen (LN 2 ) level for 20 min and dumping into LN 2 pending thawing (Martins et al 2009). Approximately 1 week after freezing, the straws were thawed in 70°C water for 6 s. The thawed sperm that were previously retrieved from the epididymides by Tris were allocated to three aliquots for being further diluted with: (I) nothing (Tris-O), (II) Tris (Tris-Tris) and (III) TALP (Tris-TALP).…”

Section: Experimental Designmentioning

confidence: 99%

“…In addition, recovery of sperm from minced epididymides using Tris-fructose citrate solution plus 20% egg yolk resulted in the motility and viability of retrieved sperm of ≥60% (Tsutsui et al 2003). Likewise, the motility and membrane integrity of epididymal cat sperm, recovered by squeezing the epididymides, diluted with egg yolk-Tris-glucose solution were as good as 80% (Martins et al 2009). Very recently, Jimenez et al (2013) compared the efficacy of Tris-egg yolk-based extenders, between non-feline commercial (Triladyl) and Tris-glucose/fructose citrate solutions, for the cryopreservation of epididymal cat sperm.…”

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

“…Likewise, the motility and membrane integrity of epididymal cat sperm, recovered by squeezing the epididymides, diluted with egg yolk–Tris–glucose solution were as good as 80% (Martins et al. ). Very recently, Jimenez et al.…”

Section: Introductionmentioning

confidence: 99%

“…Epididymal domestic cat (Felis catus) sperm have been widely used as a model for studying various aspects of sperm retrieved from the epididymides of wild felines that died unexpectedly (Farstad 2000;Martins et al 2009;Chatdarong et al 2010a,b). In many of those studies, Tris-based solutions were used as extenders for recovering sperm cells from the epididymides and/or diluting retrieved epididymal sperm for further processes such as cooling (4°C), freezing (À196°C) and thawing (Tsutsui et al 2003;Martins et al 2009;Chatdarong et al 2010a,b). Using Tris-glucose-citrate solution (a Tris buffer; herein called 'Tris') to transmigrate sperm from minced epididymides, the motility, plasma membrane integrity and acrosome integrity of sperm retrieved were approximately 60% (Chatdarong et al 2010b).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Sperm‐TALP: An Alternative Extender for Retrieving and Diluting Epididymal Sperm in the Domestic Cat

Buranaamnuay

2013

Reprod Domestic Animals

View full text Add to dashboard Cite

Effects of sperm-TALP (TALP) on the quality of fresh-extended and frozen-thawed epididymal cat sperm were evaluated. The epididymides suspended in Tris-glucose-citrate solution (Tris), a conventional medium, and TALP were cut into small pieces to recover epididymal sperm. In experiment 1, the sperm pellets remained after centrifugation were re-suspended (1 : 2, v/v) in Tris and TALP. The sperm quality in all four groups, that is, sperm retrieved with Tris (I and II) or TALP (III and IV) and diluted with Tris (I and III) or TALP (II and IV) was assessed. The sperm motility at the 0-h incubation in TALP-TALP was superior to that of the rest (p < 0.001 to p = 0.04). At the 2-h incubation, the motility in Tris/TALP-TALP was greater than that in Tris/TALP-Tris (p ≤ 0.001). In experiment 2, after centrifugation, the sperm pellets were added with freezing extenders and frozen. The thawed sperm previously retrieved from the epididymides with Tris and TALP were allotted so as not to further diluted (Tris/TALP-O) and to further diluted (1 : 1, v/v) with Tris (Tris/TALP-Tris) and TALP (Tris/TALP-TALP) and were evaluated the quality. At both incubation times, the motility of frozen-thawed sperm recovered with TALP (TALP-O/Tris/TALP) was comparable with or significantly higher than that in the Tris groups (Tris-O/Tris/TALP; p = 0.003 to p > 0.05). The motility and viability of thawed sperm in Tris-Tris were significantly decreased during the 2-h incubation (p = 0.007 for the motility and p = 0.01 for the viability). In both experiments, neither type of diluent (Tris vs TALP) nor incubation time (0 vs 2 h) significantly affected the sperm membrane integrity under hypo-osmotic condition (p > 0.05). According to beneficial effects on the quality of fresh-extended and frozen-thawed sperm demonstrated, sperm-TALP could be used as an alternative medium for recovering sperm from the epididymides and for diluting epididymal sperm in the domestic cat.

show abstract

Section: Experimental Designmentioning

confidence: 99%