Impact of a high hydrostatic pressure pretreatment on the separation of bioactive peptides from flaxseed protein hydrolysates by electrodialysis with ultrafiltration membranes

Marie, Gina Cecile Urbain; Perreault, Véronique; Hénaux, Loïc; Carnovale, Valérie; Aluko, Rotimi E.; Marette, André; Doyen, Alain; Bazinet, Laurent

doi:10.1016/j.seppur.2018.09.063

Cited by 30 publications

(8 citation statements)

References 35 publications

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“…They observed that the peptide sequence ALPMHIR, identified as lactokinin and known to exert an important antihypertensive effect, was recovered with an estimated 66% migration rate [ 241 ] and that the peptide migration rate was found to be affected by the mode of enzymatic hydrolysis and separation [ 242 ]. Current research is exploring other ways to optimize hydrolysis before EDUF, e.g., by using high hydrostatic pressure [ 243 ]. Recently, Wang et al [ 244 ] synthesized a polyvinylalcohol (PVA) membrane aiming at a 150-kDa MWCO for use as a filtration membrane in an EDUF stack.…”

Section: Recent Technological Developments Based On Ed Membrane Phmentioning

confidence: 99%

Electrodialytic Processes: Market Overview, Membrane Phenomena, Recent Developments and Sustainable Strategies

Bazinet

Geoffroy

2020

Membranes

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In the context of preserving and improving human health, electrodialytic processes are very promising perspectives. Indeed, they allow the treatment of water, preservation of food products, production of bioactive compounds, extraction of organic acids, and recovery of energy from natural and wastewaters without major environmental impact. Hence, the aim of the present review is to give a global portrait of the most recent developments in electrodialytic membrane phenomena and their uses in sustainable strategies. It has appeared that new knowledge on pulsed electric fields, electroconvective vortices, overlimiting conditions and reversal modes as well as recent demonstrations of their applications are currently boosting the interest for electrodialytic processes. However, the hurdles are still high when dealing with scale-ups and real-life conditions. Furthermore, looking at the recent research trends, potable water and wastewater treatment as well as the production of value-added bioactive products in a circular economy will probably be the main applications to be developed and improved. All these processes, taking into account their principles and specificities, can be used for specific eco-efficient applications. However, to prove the sustainability of such process strategies, more life cycle assessments will be necessary to convince people of the merits of coupling these technologies.

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Section: Recent Technological Developments Based On Ed Membrane Phmentioning

confidence: 99%

Electrodialytic Processes: Market Overview, Membrane Phenomena, Recent Developments and Sustainable Strategies

Bazinet

Geoffroy

2020

Membranes

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“…Thus, sequence integration of EDUF-NF would be advantageous to refine the UF permeate [99]. Second, pretreatment of defatted flaxseed protein isolate with high hydrostatic pressure (HHP) at 400 MPa HHP and 21 • C for 20 min, and further processing with EDUF resulted in isolate (recovered in KCl) rich in arginine, which lowers systolic blood pressure [100]. Arginine itself cannot be separated using conventional electrodialysis since it adulterated the CEM in basic EDUF [101].…”

Section: Electroultrafiltration (Euf) and Electrodialysis With Uf Memmentioning

confidence: 99%

Recent Advancements of UF-Based Separation for Selective Enrichment of Proteins and Bioactive Peptides—A Review

et al. 2021

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Proteins are one of the primary building blocks that have significant functional properties to be applied in food and pharmaceutical industries. Proteins could be beneficial in their concentrated products or isolates, of which membrane-based filtration methods such as ultrafiltration (UF) encompass application in broad spectra of protein sources. More importantly, selective enrichment by UF is of immense interest due to the presence of antinutrients that may dominate their perspicuous bioactivities. UF process is primarily obstructed by concentration polarization and fouling; in turn, a trade-off between productivity and selectivity emerges, especially when pure isolates are an ultimate goal. Several factors such as operating conditions and membrane equipment could leverage those pervasive contributions; therefore, UF protocols should be optimized for each unique protein mixture and mode of configuration. For instance, employing charged UF membranes or combining UF membranes with electrodialysis enables efficient separation of proteins with a similar molecular weight, which is hard to achieve by the conventional UF membrane. Meanwhile, some proposed strategies, such as utilizing ultrasonic waves, tuning operating conditions, and modifying membrane surfaces, can effectively mitigate fouling issues. A plethora of advancements in UF, from their membrane material modification to the arrangement of new configurations, contribute to the quest to actualize promising potentials of protein separation by UF, and they are reviewed in this paper.

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“…Durand et al [45] found that the anionic fraction obtained after the 50 kg mol −1 MWCO membrane had the highest antioxidant activity whereas anti-inflammatory activities were higher in the cationic fractions collected after the first and second membranes. EDUF using 20 kg mol −1 MWCO UF membranes enabled the separation of arginine-containing peptides in a defatted flaxseed protein hydrolysate, enhancing hypotension effects in vivo of fractions, since these effects have been associated with the presence of arginine in active peptides [47]. EDUF has recently been considered for large scale peptide production in substitution to several chromatographic operations.…”

Section: Peptide Separation and Purification By Chargementioning

confidence: 99%

“…A positively charged antimicrobial peptide, obtained from hemoglobin, was enriched 24-fold using a 10 kg mol −1 MWCO membrane [44]. Associated costs of producing peptides from EDUF technologies was reported to range from 0.3 to 0.5 Canadian dollars per gram of peptides for an effective filtration area of 10 m 2 [47]. These results indicate the potential of this technology in the field of recovery of peptides from by-products, but competitive evaluation was not done because the cost of peptide recovery using other separation techniques was not yet reported to our best knowledge (e.g., ultrafiltration or chromatography).…”

Section: Peptide Separation and Purification By Chargementioning

confidence: 99%

Membrane Fractionation of Protein Hydrolysates from By-Products: Recovery of Valuable Compounds from Spent Yeasts

et al. 2020

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Spent brewer’s yeast (Saccharomyces sp.), the second most generated by-product from the brewing industry, contains bioactive and nutritional compounds with high added value such as proteins (40–50%), polysaccharides, fibers and vitamins. Molecules of interest from agro-industrial by-products need to be extracted, separated, concentrated, and/or purified so that a minimum purity level is achieved, allowing its application. Enzymatic hydrolysis has been successfully used in the production of peptides and protein hydrolysates. The obtained hydrolysates require efficient downstream processes such as membrane technology, which is an important tool for the recovery of thermolabile and sensitive compounds from complex mixtures, with low energy consumption and high specificity. The integration of membrane techniques that promote the separation through sieving and charge-based mechanisms is of great interest to improve the purity of the recovered fractions. This review is specifically addressed to the application of membrane technologies for the recovery of peptides from yeast protein hydrolysates. Fundamental concepts and practical aspects relative to the ultrafiltration of agro-industrial protein hydrolysates will be described. Challenges and perspectives involving the recovery of peptides from yeast protein hydrolysates will be presented and thoroughly discussed.

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Impact of a high hydrostatic pressure pretreatment on the separation of bioactive peptides from flaxseed protein hydrolysates by electrodialysis with ultrafiltration membranes

Cited by 30 publications

References 35 publications

Electrodialytic Processes: Market Overview, Membrane Phenomena, Recent Developments and Sustainable Strategies

Electrodialytic Processes: Market Overview, Membrane Phenomena, Recent Developments and Sustainable Strategies

Recent Advancements of UF-Based Separation for Selective Enrichment of Proteins and Bioactive Peptides—A Review

Membrane Fractionation of Protein Hydrolysates from By-Products: Recovery of Valuable Compounds from Spent Yeasts

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