Impact of Agrobacterium tumefaciens-induced stem tumors on NO 3 − uptake in Ricinus communis

Mistrı́k, Igor; Pavlovkin, Ján; Wächter, Rebecca; Pradel, Katja S.; Schwalm, Katja; Hartung, Wolfram; Mathesius, Ulrike; Stöhr, Christine; Ullrich, C.

doi:10.1007/978-94-017-2858-4_15

Cited by 8 publications

(19 citation statements)

References 32 publications

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“…Like in other species, tumors of Arabidopsis accumulate high levels of the stress hormone ABA (Mistrik et al, 2000;Veselov et al, 2003;Wächter et al, 2003). Accordingly, we identified ABA-dependent adaptive mechanisms on the level of gene regulation, as well as accumulation of osmoprotectants.…”

Section: Aba Induces Drought Adaptations and Regulates Water Flow Intmentioning

confidence: 53%

“…High levels of ABA levels are found in Arabidopsis stalks above the rosette, as well as in tumor tissue close to vascular bundles. Thus, ethylene-induced ABA production in leaves might result in its transport via the phloem from leaves into tumors (Mistrik et al, 2000).…”

Section: Aba Induces Drought Adaptations and Regulates Water Flow Intmentioning

confidence: 99%

“…Expression of the T-DNAencoded oncogenes in turn promote biosynthesis of auxin and cytokinin, resulting in non-plant-controlled production of these phytohormones. This initiates cell proliferation as well as differentiation of a vascular network, which will connect the tumor to vascular bundles of the host plant and thus support the tumor with nutrients and water (Kado, 1984;Aloni et al, 1995;Mistrik et al, 2000;Ullrich and Aloni, 2000).…”

mentioning

confidence: 99%

“…In contrast to previous studies (e.g. Mistrik et al, 2000;Veselov et al, 2003;Wächter et al, 2003) we took advantage of the genetic model plant Arabidopsis to dissect the role of ABA signaling in drought protection during crown gall development. We applied microarray techniques to investigate expression of the wellknown marker genes for drought stress and/or ABA.…”

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confidence: 99%

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A Central Role of Abscisic Acid in Drought Stress Protection of Agrobacterium-Induced Tumors on Arabidopsis

Efetova¹,

Zeier²,

Riederer³

et al. 2007

Plant Physiology

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Crown gall tumors induced by Agrobacterium tumefaciens represent a sink that has to be provided with nutrients and water by the host plant. The lack of an intact epidermis or cuticle results in uncontrolled loss of water. However, neither the tumor nor the host plant displays wilting. This phenomenon points to drought adaptation in both tumors and the crown gall host plant. To understand the underlying molecular mechanisms of protection against desiccation the gene expression pattern of Arabidopsis (Arabidopsis thaliana) tumors was integrated with the profile of stress metabolites: Arabidopsis tumors accumulated high amounts of abscisic acid (ABA), the ethylene precursor aminocyclopropyl carboxylic acid, osmoprotectants, and form a suberized periderm-like protective layer. Suberization of the outer tumor cell layers most likely is mediated by ABA since external application of ABA induced suberization of Arabidopsis roots. However, the expression level of the classical marker genes, known to respond to drought stress and/or ABA, was lower in tumors. Instead another set of drought and/or ABAinducible genes was more highly transcribed. Elevated transcription of several ABA-dependent aquaporin genes might indicate that ABA controls the water balance of the tumor. The retarded tumor growth on abi and aba mutant plants underlined the importance of a tumor-specific ABA signaling pathway. Taken together, we propose that ABA is an important signal for protection of tumors against desiccation and thus supports tumor development.

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Section: Aba Induces Drought Adaptations and Regulates Water Flow Intmentioning

confidence: 53%

Section: Aba Induces Drought Adaptations and Regulates Water Flow Intmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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A Central Role of Abscisic Acid in Drought Stress Protection of Agrobacterium-Induced Tumors on Arabidopsis

Efetova¹,

Zeier²,

Riederer³

et al. 2007

Plant Physiology

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“…Rapid growth of plant tumors creates strong metabolic sinks on host plants (Malsy et al, 1992;Pradel et al, 1996Pradel et al, , 1999Mistrik et al, 2000). In Arabidopsis tumors, almost all major nutrients were at higher concentrations than in the respective host tissues (Figures 6 to 8).…”

Section: Nutrient Accumulationmentioning

confidence: 99%

An Integrated View of Gene Expression and Solute Profiles of Arabidopsis Tumors: A Genome-Wide Approach

et al. 2006

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Transformation of plant cells with T-DNA of virulent agrobacteria is one of the most extreme triggers of developmental changes in higher plants. For rapid growth and development of resulting tumors, specific changes in the gene expression profile and metabolic adaptations are required. Increased transport and metabolic fluxes are critical preconditions for growth and tumor development. A functional genomics approach, using the Affymetrix whole genome microarray (∼22,800 genes), was applied to measure changes in gene expression. The solute pattern of Arabidopsis thaliana tumors and uninfected plant tissues was compared with the respective gene expression profile. Increased levels of anions, sugars, and amino acids were correlated with changes in the gene expression of specific enzymes and solute transporters. The expression profile of genes pivotal for energy metabolism, such as those involved in photosynthesis, mitochondrial electron transport, and fermentation, suggested that tumors produce C and N compounds heterotrophically and gain energy mainly anaerobically. Thus, understanding of gene-to-metabolite networks in plant tumors promotes the identification of mechanisms that control tumor development.

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Development of Agrobacterium tumefaciens C58-induced plant tumors and impact on host shoots are controlled by a cascade of jasmonic acid, auxin, cytokinin, ethylene and abscisic acid

Веселов¹,

Langhans

Hartung

et al. 2003

Planta

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The development of Agrobacterium tumefaciens-induced plant tumors primarily depends on the excessive production of auxin and cytokinin by enzymes encoded on T-DNA genes integrated into the plant genome. The aim of the present study was to investigate the involvement of additional phytohormone signals in the vascularization required for rapid tumor proliferation. In stem tumors of Ricinus communis L., free auxin and zeatin riboside concentrations increased within 2 weeks to 15-fold the concentrations in control stem tissue. Auxin and cytokinin immunolocalization revealed the highest concentrations within and around tumor vascular bundles with concentration gradients. The time-course of changes in free auxin concentration in roots was inversely correlated with that in the tumors. The high ethylene emission induced by increased auxinand cytokinin correlated with a 36-fold accumulation of abscisic acid in tumors. Ethylene emitted from tumors and exogenously applied ethylene caused an increase in abscisic acid concentrations also in the host leaves, with a diminution in leaf water vapor conductance. Jasmonic acid concentration reached a maximum already within the first week of bacterial infection. A wound effect could be excluded. The results demonstrate the concerted interaction of a cascade of transiently induced, non-T-DNA-encoded phytohormones jasmonic acid, ethylene and abscisic acid with T-DNA-encoded auxin and zeatin riboside plus trans-zeatin, all of which are required for successful plant tumor vascularization and development together with inhibition of host plant growth. Electronic Supplementary Material is available if you access this article at http://dx.

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Impact of Agrobacterium tumefaciens-induced stem tumors on NO 3 − uptake in Ricinus communis

Cited by 8 publications

References 32 publications

A Central Role of Abscisic Acid in Drought Stress Protection of Agrobacterium-Induced Tumors on Arabidopsis

A Central Role of Abscisic Acid in Drought Stress Protection of Agrobacterium-Induced Tumors on Arabidopsis

An Integrated View of Gene Expression and Solute Profiles of Arabidopsis Tumors: A Genome-Wide Approach

Development of Agrobacterium tumefaciens C58-induced plant tumors and impact on host shoots are controlled by a cascade of jasmonic acid, auxin, cytokinin, ethylene and abscisic acid

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