Nowadays more and more emphasis is placed on the protection of the natural environment. Scientists notice that global warming is associated with an increase of carbon dioxide emissions, which results inter alia from the combustion of gasoline, oil, and coal. To reduce the problem of pollution from transport, the EU is introducing increasingly stringent emission standards which should correspond to sustainable conditions of the environment during the operation of motor vehicles. The emissivity value of substances, such as nitrogen oxides (NOx), hydrocarbons (HC), carbon monoxide (CO), as well as solid particles, was determined. The aim of this paper was to examine, by means of simulation in the Scilab program, the exhaust emissions generated by the 1.3 MultiJet Fiat Panda diesel engine, and in particular, carbon monoxide and nitrogen oxides (verified on the basis of laboratory tests). The Fiat Panda passenger car was selected for the test. The fuels supplied to the tested engine were diesel and FAME (fatty acid methyl esters). The Scilab program, which simulated the diesel engine operation, was the tool for analyzing the exhaust toxicity test. The combustion of biodiesel does not necessarily mean a smaller amount of exhaust emissions, as could be concluded on the basis of information contained in the subject literature. The obtained results were compared with the currently valid EURO-6 standard, for which the limit value for CO is 0.5 g/km, and for NOx − 0.08 g/km, and it can be seen that the emission of carbon monoxide did not exceed the standards in any case examined. Unfortunately, when analyzing the total emissions of nitrogen oxides, the situation was completely the opposite and the emissions were exceeded by 20–30%.