Impact of anti-inflammatory agent like Bryophyllum pinnatum aqueous extract and diclofenac on blood pressure and creatinine clearance.

Abstract

Objectives: To study the impact of anti-inflammatory agent like Bryophyllum pinnatum aqueous extract and diclofenac on blood pressure and creatinine clearance. Study Design: Experimental study. Setting: Sargodha Medical College, Sargodha, Department of Pharmacy, University of Sargodha. Period: 1st January 2019 to 30th June 2019. Material & Methods: 24 Sprague Dawley rats were obtained and were then divided into four groups. Negative control group (A) contained animals received normal diet while Group B, C and D received diet containing sucrose (20% w/w) to induce hypertension. After that group B (positive control group) received distilled water 0.5 ml was by oral route, group C (Bryophyllum pinnatum group) received Bryophyllum pinnatum aqueous extract 300 mg/ml intraperitoneally and group D received diclofenac 12 mg/kg intraperitoneally as a single morning dose for two weeks. Blood pressure of animals was recorded at baseline and then weekly throughout the study using tail cuff using non-invasive blood pressure controller (ML125R). Animals were anesthetised with chloroform and two ml blood was drawn through cardiac puncture at 0, 4and 6 weeks. Blood was tested for haematocrit. Serum sodium and potassium levels were estimated by flame photometer. Urinary creatinine levels were estimated by kinetic Jaffé method. The data collected was processed by using Statistical Package for Social Sciences (SPSS 20). Results: Mean and standard deviation of systolic blood pressure (BP) of group A rats did not change with time, while that of group B, C and D increased till 4th week. Creatinine clearance of group A, B and C did not change much with time but that of group D decreased towards end of study period. Conclusion: Bryophyllum pinnatum leaf aqueous extract is an effective anti-hypertensive agent with minimal renal effects.

Comparative Evaluation of Aloe vera and Diclofenac on Body Weight, Blood Pressure and Renal Function of Hypertensive Rats

“…In accordance with this study, intra-peritoneal injection of diclofenac has shown a significant (p <0.001) rise in blood pressure of hypertensive rats. 20 In another study, diclofenac has shown increase in blood pressure of elderly human volunteers with a p value of 0.08. 21 These results are contrary to a study in which diclofenac did not change blood pressure of hypertensive rats 22 which may be because of a very small dose (1mg/kg) of diclofenac given for fifteen days.…”

“…26 Another study has shown similar non-significant decrease in creatinine clearance in rats after diclofenac treatment. 20 Had the study been conducted for longer duration, results of serum creatinine and creatinine clearance may have become significant.…”

Repurposing of Bryophyllum pinnatum for dysmenorrhea treatment: a systematic scoping review and case series

“…In these studies, leaf extract as well as root extract inhibited xanthinoxidase ( Latif et al, 2020 ; Morais Fernandes et al, 2021 ). 3-O-α-L-Arabinopyranosyl-(1→ 2)-O-α-L-rhamnopyranoside was shown to inhibit phosphodiesterase 4 (PDE4) ( Lourenço et al, 2020 ) and the antiplatelet effect of the aqueous extract indicated an effect on the arachidonic acid pathway (as does the inhibition of COX) ( Naqvi et al, 2019 ).…”