Impact of Bacillus on Phthalides Accumulation in Angelica sinensis (Oliv.) by Stoichiometry and Microbial Diversity Analysis

Abstract

Plant-microorganism interaction in the rhizosphere is thought to play an important role in the formation of soil fertility, transformation and absorption of nutrients, growth and development of medicinal plants, and accumulation of medicinal ingredients. Yet, the role that they play in the phthalides accumulation of Angelica sinensis (Oliv.) Diels remains unclear. In the present study, we report a correlative analysis between rhizosphere microorganisms and phthalides accumulation in A. sinensis from Gansu, China where was the major production areas. Meanwhile, Bacillus was explored the potential functions in the plant growth and phthalide accumulation. Results revealed that the common bacterial species detected in six samples comprised 1150 OTUs which were involved in 368 genera, and predominant taxa include Actinobacteria, Acidobacteria, and Proteobacteria. The average contents of the six phthalides were 4.0329 mg/g. The correlation analysis indicated that 20 high abundance strains showed positive or negative correlations with phthalides accumulation. Flavobacterium, Nitrospira, Gaiella, Bradyrhizobium, Mycobacterium, Bacillus, RB41, Blastococcus, Nocardioides, and Solirubrobacter may be the key strains that affect phthalides accumulation on the genus level. By the plant-bacterial co-culture and fermentation, Bacillus which were isolated from rhizosphere soils can promote the plant growth, biomass accumulation and increased the contents of the butylidenephthalide (36∼415%) while the ligustilide (12∼67%) was decreased. Altogether, there is an interaction between rhizosphere microorganisms and phthalides accumulation in A. sinensis, Bacillus could promote butylidenephthalide accumulation while inhibiting ligustilide accumulation.

Explore the interaction between root metabolism and rhizosphere microbiota during the growth of Angelica sinensis

“…The root microbial community could affect the growth and yield of A. sinensis . Studies have shown that Bacillus isolated from the rhizosphere can promote biomass accumulation and plant growth, and can increase the contents of butylidenephthalide (36 ~ 415%) of A. sinensis ( Feng et al., 2020 ). Nonetheless, little is known about the mechanisms of the accumulation of A. sinensis metabolites and the microbial communities.…”

Investigation of Enzymes in the Phthalide Biosynthetic Pathway in Angelica sinensis Using Integrative Metabolite Profiles and Transcriptome Analysis

“…Standard and sample solutions were prepared using an established method, as described previously (Feng et al, 2021). Chromatographic analyses were performed using a Waters Acquity UPLC system (Waters Corp., MA, United States), whereas mass spectromet was conducted using an AB SCIEX Triple Quad 6,500 plus (AB SCIEX Corp., Framingham, MA, United States) with electrospray ionization.…”

“…Raw data were processed using MultiQuant v3.0.2 (AB SCIEX Corp.). A detailed description of the standard solution, chromatographic conditions, and methodology validation were presented in our previous study (Feng et al, 2021).…”

“…The most selective and specific transition was chosen for multiple reaction monitoring (MRM) determination, and all the MRM parameters are provided in Supplementary Table S3. The UHPLC method was validated by assessing the linearity, precision, stability, limit of detection, limit of quantification, and recovery (Feng et al, 2021).…”

Diversity and structural analysis of rhizosphere soil microbial communities in wild and cultivated Rhizoma Atractylodis Macrocephalae and their effects on the accumulation of active components

“…Pseudomonas, Bacillus and Mycorrhizal fungi which were isolated from the rhizosphere, could promote the production of secondary metabolites ( Lugtenberg & Kamilova, 2009 ). In the previous study, the correlation between angelica rhizosphere microorganisms and the accumulation of phthalic acid compounds was analyzed, and it had been revealed that Bacillus could promote the accumulation of butene phthalides ( Feng et al, 2021 ). It was of great significance to study the rhizosphere microorganisms of medicinal plants and their impacts on the accumulation of active ingredients in these medicinal plants.…”