Impact of bacteria on aggregation of crystalline and amorphous components of infectious urinary stones

Prywer, Jolanta; Torzewska, Agnieszka

doi:10.1016/j.jcrysgro.2018.10.018

Cited by 4 publications

(23 citation statements)

References 31 publications

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“…In addition, our results presented in ref. 20 demonstrate that in the presence of bacteria an increased aggregation process of PCaAP is observed. Experimental results indicate that among the macromolecules released from Proteus mirabilis (P. mirabilis) cells, lipopolysaccharide (LPS) is responsible for increased aggregation of PCaAP 20 .…”

Section: Introductionmentioning

confidence: 65%

“…20 demonstrate that in the presence of bacteria an increased aggregation process of PCaAP is observed. Experimental results indicate that among the macromolecules released from Proteus mirabilis (P. mirabilis) cells, lipopolysaccharide (LPS) is responsible for increased aggregation of PCaAP 20 . LPS is a main component of the outer membrane in Gram-negative bacteria and plays a role in their protection from adverse environmental conditions and it is an important virulence factor.…”

Section: Introductionmentioning

confidence: 65%

“…The further course of action with artificial urine is the same as procedure described in ref. 20 . This means, among other things, that the artificial urine was filtered using a membrane filter with pore size of 0.2 μm.…”

Section: Methodsmentioning

confidence: 99%

“…The literature review shows that researchers conduct LPS studies in different contexts, and the most commonly used is free LPS suspended in a suitable solvent over a wide concentration range from 2 μg/ml to 500 μg/ml 29–31 . In our previous work 20 to assess the effect of P. mirabilis on PCaAP aggregation, we investigated the aggregation of PCaAP in the presence of bacterial macromolecules with and without LPS. In the case of bacterial macromolecules with LPS, the concentration of LPS was in the range 20–40 μg/ml.…”

Section: Introductionmentioning

confidence: 99%

“…In the light of the above review of the literature and the results obtained by us 18,20 , the aim of the present study is to analyze the effect of LPS isolated from the P. mirabilis strain C11 on PCaAP aggregation and verify its ability to influence aggregation processes. LPS isolated from P. mirabilis was chosen for analysis because this species is isolated from human infectious urinary stones in 70% cases 32,33 .…”

Section: Introductionmentioning

confidence: 99%

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Aggregation of poorly crystalline and amorphous components of infectious urinary stones is mediated by bacterial lipopolysaccharide

Prywer

Torzewska

2019

Sci Rep

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Poorly crystalline and amorphous precipitate (PCaAP) is one of the components of the so-called infectious urinary stones, which are the result of the activity of urease-producing microorganisms, mainly from the Proteus species, in particular Proteus mirabilis. The main component of this kind of stones is crystalline struvite (MgNH4PO4∙6H2O). Bacteria can build into the structure of the urinary stone and, in this way, they are one of the components of the urinary stone. From these three components – PCaAP, struvite and Proteus mirabilis – PCaAP exhibits the greatest ability to aggregate. The present study focuses on the aggregation of PCaAP. In particular, an influence of lipopolysaccharide (LPS) isolated from Proteus mirabilis on aggregation of PCaAP is presented. An aggregation of PCaAP is characterized by cross-sectional area of aggregates and zeta potential. The results demonstrate that, in artificial urine, the influence of freely suspended LPS on aggregation of PCaAP depends on the concentrations of LPS. Small concentrations of freely suspended LPS enhance the aggregation of PCaAP compared to the control test. For high concentrations of freely suspended LPS the formation of aggregates of PCaAP is inhibited. LPS, which is not freely suspended, but covers polystyrene latex beads, has no such properties. The investigations provide evidence for the importance of biological regulation in the PCaAP aggregation process.

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Section: Introductionmentioning

confidence: 65%

Section: Introductionmentioning

confidence: 65%

Section: Methodsmentioning

confidence: 99%