Impact of Caloric Vestibular Stimulation on Co-Ordination in Parkinson Disease Induced Mice

Mohan, Ram; KAYALVIZHI,; Ramanathan, Rashmi; Shanmugam, Jeevithan; Archana, R

doi:10.22159/ijpps.2022v14i10.45523

Cited by 1 publication

(1 citation statement)

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“…Due to the clinical failures of previously identified drug candidates, there is an urgent need for the development of new and more effective PD treatments [3]. Sirtuin 3 (SIRT3), a well-known mitochondrial deacetylase, is involved in mitochondrial function and metabolism under various stress conditions, resulting in the downregulation of reactive oxygen species (ROS) [4]. SIRT3 is essential for controlling metabolism, autophagy, cell death, inflammatory responses, and ATP generation [5].…”

Section: Introductionmentioning

confidence: 99%

In Silico, Preparation and in Vitro Studies of Benzylidene-Based Hydroxy Benzyl Urea Derivatives as Free Radical Scavengers in Parkinson’s Disease

CHAND,

KANDY,

PRASAD

et al. 2024

Int J App Pharm

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Objective: The study focuses on the benzylidene-based hydroxy benzyl urea derivative as free radical scavengers in PD. Methods: The derivatives were designed, synthesized, and characterized using FTIR, 1H, 13C-NMR, and Mass spectrometry. Further in vitro studies were performed on the SHSY-5Y cell lines. Molecular docking and molecular dynamic studies were performed at 100 ns to predict the binding affinity and stability of the ligand/protein complex. Results: Among the nine derivatives, compounds HBU-2, and HBU-4were found to have the highest binding affinity-9.699 kcal/mol, and-9.020 kcal/mol with the amino acid interactions SER 149, PHE 157, ARG 158, SER 159, ILE 230, and ASP 231. Further, this HBU-1 to HBU-9 derivatives were produced using a synthesis route. The neurotoxicity studies were performed on the SHSY-5Y cells, where the % cell viability for the compound HBU-2, and HBU-4 was 91.22 %, and 90.42 %at a minimal concentration of 125 µg/ml with a p-value<0.011. Further, the cell counts and LDH assay for the compound HBU-2, and HBU-4 with MPP+treatment predicted 0.72-fold change and 0.66-fold change. The ROS % activity was also measured for compounds HBU-2 and HBU-4 in conjunction with the MPP+induction. In the SHSY-5Y cell line, compound HBU-2 downregulated the ROS level to 45%. Conclusion: The synthesized compounds were found to have good free radical scavenging properties on SHSY-5Y neuroblastoma cell lines, considering these derivatives could be further assessed using appropriate PD models.

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Section: Introductionmentioning

confidence: 99%