Impact of Chemical and Alternative Fungicides Applied to Grapevine cv Nebbiolo on Microbial Ecology and Chemical-Physical Grape Characteristics at Harvest

Rantsiou, Kalliopi; Giacosa, Simone; Pugliese, Massimo; Englezos, Vasileios; Ferrocino, Ilario; Monchiero, M.; Gribaudo, Ivana; Gambino, Giorgio; Gullino, M. L.

doi:10.3389/fpls.2020.00700

Cited by 39 publications

(61 citation statements)

References 74 publications

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“…Five commercial antifungal compounds, i.e., fosetyl-Al (Fos-Al), potassium phosphonate (K-Pho), laminarin (Lam), electrolyzed water (EOW) and metiram (Met), were applied in 2017 in a vineyard of ‘Nebbiolo’ following the scheme reported in Table S1 and previously described [ 22 ]. The antifungal compounds were used in combination with sulphur and copper since the experiment was conducted in a commercial vineyard and it was necessary to follow the legal rules for antifungal treatments.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, the results obtained refer to the whole treatment used in the vineyard ( Table S1 ) rather than to the single antifungal compound. 58% of clusters in the untreated control (CTRL) were affected by U. necator , all other treatments reduced the levels of powdery mildew infection and no symptoms associated to downy mildew were observed [ 22 ]. Lam and K-Pho were among the treatments showing the best control of the pathogen, followed by Fos-Al and Met, while EOW induced a very limited control of powdery mildew [ 22 , 27 ].…”

Section: Resultsmentioning

confidence: 99%

“…Grapevine represents a model for studying interactions among plant, elicitors and pathogens [ 17 , 18 ]. Despite the growing interest in the use of these alternative products, few studies were conducted for the development and application of these solutions [ 19 , 20 , 21 , 22 ]. Additional efforts should be done to assess the effects of these strategies on the production and quality of grapes and wine, providing information useful for the companies acting in this sector.…”

Section: Introductionmentioning

confidence: 99%

“…Additional efforts should be done to assess the effects of these strategies on the production and quality of grapes and wine, providing information useful for the companies acting in this sector. Recently, the efficacy of environmentally friendly products against downy and powdery mildews have been demonstrated in a vineyard of ‘Nebbiolo,’ a grape variety commonly used for production of renowned Barolo, Barbaresco and Roero PDO wines [ 22 ]. ‘Nebbiolo,’ as well as all varieties of V. vinifera , is very sensitive to attacks by fungi and oomycota in field conditions.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, it is particularly sensitive to powdery mildew more than other genotypes [ 18 ]. Rantsiou et al [ 22 ] has already verified the impact of these compounds (e.g., laminarin and chito-oligosaccharides) on yield and vine vigor, skin features (including phenolic composition) and yeast community present on grape berries at harvest. Particularly, laminarin, which is extracted from the brown algae Laminaria digitate , is already known to act inducing resistance against downy and powdery mildew and grey mould in grapevine [ 23 , 24 , 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Long-Term Impact of Chemical and Alternative Fungicides Applied to Grapevine cv Nebbiolo on Berry Transcriptome

Balestrini

Ghignone

Quiroga

et al. 2020

IJMS

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Viticulture is one of the horticultural systems in which antifungal treatments can be extremely frequent, with substantial economic and environmental costs. New products, such as biofungicides, resistance inducers and biostimulants, may represent alternative crop protection strategies respectful of the environmental sustainability and food safety. Here, the main purpose was to evaluate the systemic molecular modifications induced by biocontrol products as laminarin, resistance inducers (i.e., fosetyl-Al and potassium phosphonate), electrolyzed water and a standard chemical fungicide (i.e., metiram), on the transcriptomic profile of ‘Nebbiolo’ grape berries at harvest. In addition to a validation of the sequencing data through real-time polymerase chain reaction (PCR), for the first-time the expression of some candidate genes in different cell-types of berry skin (i.e., epidermal and hypodermal layers) was evaluated using the laser microdissection approach. Results showed that several considered antifungal treatments do not strongly affect the berry transcriptome profile at the end of season. Although some treatments do not activate long lasting molecular defense priming features in berry, some compounds appear to be more active in long-term responses. In addition, genes differentially expressed in the two-cell type populations forming the berry skin were found, suggesting a different function for the two-cell type populations.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Long-Term Impact of Chemical and Alternative Fungicides Applied to Grapevine cv Nebbiolo on Berry Transcriptome

Balestrini

Ghignone

Quiroga

et al. 2020

IJMS

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Novel sustainable strategies to control Plasmopara viticola in grapevine unveil new insights on priming responses and arthropods ecology

Giudice

Moffa

Niero

et al. 2022

Pest Management Science

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BACKGROUND Reduction of fungicide consumption in agriculture is globally recognized as a priority. Government authorities are fostering research to achieve a reduction of risks associated with conventional pesticides and promoting the development of sustainable alternatives. To address these issues, in the present study, alternative protocols for the control of downy mildew infection in grapevine were compared to the standard protocol. In the first protocol, only resistance inducers were used, comprising a single formulation with Acibenzolar S‐methyl, laminarin and disodium‐phosphonate. The second and third protocols followed the standard protocol but substituted phosphonates with phosphorus pentoxide and Ecklonia maxima extract. RESULTS The results showed that at veraison downy mildew incidence and severity in all tested protocols were significantly reduced compared to nontreated controls on both canopy and bunches. Expression analysis of key genes involved in plant stress response, indicated that the two protocols for phosphites substitution induced a remodulation of salicylic acid (SA) and jasmonic acid (JA), with positive impact on yields. Analysis of the first protocol revealed that the primed state induced a short delay in bunch ripening, with a shift of carbohydrate metabolism to boost the plant defences, involving an upregulation of defence related‐gene, SAR response and a decreased ROS detoxification. Additionally, analysis on the arthropods populations, in parallel with the positive results achieved using alternatives to conventional fungicides, were enriched by those showing the potential of naturally occurring predators of spider mites. CONCLUSION This study provides practical solutions to reduce the environmental impact of treatments for the control downy mildew in viticulture. © 2022 Society of Chemical Industry.

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Effects of fungicide application on physiological and molecular responses of grapevine (Vitis vinifera L.): a comparison between copper and sulfur fungicides applied alone and in combination with novel fungicides

Moine

Pugliese²,

Monchiero

et al. 2023

Pest Management Science

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BACKGROUNDChemical products against fungi and oomycetes pose serious environmental issues. In the last decade, the use of less impacting active ingredients was encouraged to reduce chemical inputs in viticulture. In this study, the effect of different antifungal compounds on grapevine agronomic, physiological, and molecular responses in the vineyard was evaluated in addition to protection against powdery and downy mildews.RESULTSIn 2 years and in two Vitis vinifera cultivars (Nebbiolo and Arneis), a conventional crop protection approach, based on traditional fungicides (sulfur and copper), was compared to combined strategies. A well‐known resistance inducer (potassium phosphonate), Bacillus pumilus strain QST 2808 and calcium oxide, both active ingredients whose biological interaction with grapevine is poorly characterized, were applied in the combined strategies in association with chemical fungicides. Despite a genotype effect occurred, all treatments optimally controlled powdery and downy mildews, with minimal variations in physiological and molecular responses. Gas exchange, chlorophyll content and photosystem II efficiency increased in treated plants at the end of season, along with a slight improvement in the agronomic performances, and an activation of molecular defense processes linked to stilbene and jasmonate pathways.CONCLUSIONThe disease control strategies based on potassium phosphonate, Bacillus pumilus strain QST 2808 or calcium oxide combined with traditional chemical compounds did not cause severe limitations in plant ecophysiology, grape quality, and productive yields. The combination of potassium phosphonate and calcium oxide with traditional fungicides can represent a valuable strategy for reducing copper and sulfur inputs in the vineyards, including those organically managed. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Impact of Chemical and Alternative Fungicides Applied to Grapevine cv Nebbiolo on Microbial Ecology and Chemical-Physical Grape Characteristics at Harvest

Cited by 39 publications

References 74 publications

Long-Term Impact of Chemical and Alternative Fungicides Applied to Grapevine cv Nebbiolo on Berry Transcriptome

Long-Term Impact of Chemical and Alternative Fungicides Applied to Grapevine cv Nebbiolo on Berry Transcriptome

Novel sustainable strategies to control Plasmopara viticola in grapevine unveil new insights on priming responses and arthropods ecology

Effects of fungicide application on physiological and molecular responses of grapevine (Vitis vinifera L.): a comparison between copper and sulfur fungicides applied alone and in combination with novel fungicides

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