Impact of chlorogenic acid on modulation of significant genes in dermal fibroblasts and epidermal keratinocytes

Lee, Kyung-Ha; Do, Hwan-Kwon; Kim, Do-Yeon; Kim, Wanil

doi:10.1016/j.bbrc.2021.10.057

Cited by 28 publications

(21 citation statements)

References 24 publications

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“…This compound can also reduce lipid peroxidation, which also plays an important role in the protection of cells [ 40 ]. Other authors also report that this compound can be used in order to restore the impaired dermal matrix network, as well as the epidermal barrier [ 41 ]. Yang et al demonstrated that gallic acid can protect skin cells by increasing the expression of antioxidant genes, accelerating the migration of the keratinocytes and the fibroblasts, as well as influencing the wound-healing processes by activating the focal-adhesion kinases (FAK) and c-Jun N-terminal kinases (JNK), as well as the extracellular signal regulated kinases (Erk) [ 42 ].…”

Section: Resultsmentioning

confidence: 99%

Assessment of Cosmetic and Dermatological Properties and Safety of Use of Model Skin Tonics with Kombucha-Fermented Red Berry Extracts

Ziemlewska

Nizioł–Łukaszewska

Zagórska‐Dziok

et al. 2022

IJMS

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Kombucha is a health-promoting beverage that is produced by fermenting sweetened tea using symbiotic cultures of bacteria belonging to the genus Acetobacter, Gluconobacter, and yeast of the genus Saccharomyces. This study compared the cosmetic and dermatological properties of the extracts of the following redberries: R. rubrum, F. vesca, and R. idaeus, and their ferments, which were obtained by fermentation for 10 and 20 days using tea fungus. For this purpose, the fermented and non-fermented extracts were compared in terms of their chemical composition using the HPLC/ESI-MS chromatographic method, demonstrating the high content of biologically active compounds that were present in the ferments. The antioxidant activity of the tested samples was evaluated using DPPH and ABTS tests, as well as by evaluating the scavenging of the external and intracellular free radicals. The cytotoxicity of the extracts and the ferments, as well as the cosmetic formulations, were also determined by conducting Alamar Blue and Neutral Red tests assessing the cell viability and metabolism using skin cell lines: fibroblasts and keratinocytes. In addition, application tests were conducted showing the positive effects of the model cosmetic tonics on the TEWL, the skin hydration, and the skin pH. The results indicate that both the extracts and the ferments that were obtained from kombucha can be valuable ingredients in cosmetic products.

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Section: Resultsmentioning

confidence: 99%

Assessment of Cosmetic and Dermatological Properties and Safety of Use of Model Skin Tonics with Kombucha-Fermented Red Berry Extracts

Ziemlewska

Nizioł–Łukaszewska

Zagórska‐Dziok

et al. 2022

IJMS

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“…In our previous study, we confirmed that gentisic acid promotes proliferation through the phosphorylation of ERK1/2 in keratinocytes [ 22 ]. Syringic acid increases the expression of TGF-β and collagen 1 in keratinocyte cells [ 51 , 57 ], hesperetin is known to increase the secretion of VEGF in keratinocytes [ 58 ], and chlorogenic acid increases the proliferation of fibroblasts and increases the expression of skin barrier genes such as collagen1 and KRT1 [ 59 ]. Taken together, the keratinocyte proliferation-promoting activity of TK extract appears to be a combination of four substances, and gentisic acid and hesperetin are thought to be key substances for ERK1/2- and VEGF-related mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Trichosanthes kirilowii Extract Promotes Wound Healing through the Phosphorylation of ERK1/2 in Keratinocytes

Kim

2022

Biomimetics

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The proliferation of keratinocytes is one of the important steps in the wound-healing process, which is regulated by various signals. Prior studies have shown that Trichosanthes kirilowii extract has the ability to promote angiogenesis. Therefore, in this study, we tested the wound-healing efficacy of Trichosanthes kirilowii extract with respect to promoting keratinocyte proliferation. A total of 100 μg/mL of Trichosanthes kirilowii extract treatment improved 145.38% of keratinocyte proliferation compared with DMSO-treated control in an MTT assay and increased 238.2% of wound closure by re-epithelialization in an in vitro wound-healing assay. Trichosanthes kirilowii extract promoted ERK1/2 phosphorylation in western blot analysis and induced the expression of the c-fos and c-jun (AP-1 transcription factors), cyclins (cell cycle regulator), and growth factors CTGF and VEGF (stimulator of angiogenesis) in qRT-PCR analysis. An in vivo wound-healing assay showed that Trichosanthes kirilowii extract improved wound healing, and the significant difference in wound closure compared with DMSO-treated control was shown on days 6 and 7 with a mouse model. Taken together, we demonstrate that Trichosanthes kirilowii extract promotes the proliferation of keratinocytes by activating ERK1/2 and increasing the mRNA expression of c-fos, c-jun, CTGF, and VEGF. Therefore, we suggest Trichosanthes kirilowii extract as a new component for skin care and as a wound-healing substance.

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“…The diverse compounds in MQEF contribute significantly to the biological functions of MQEF. Chlorogenic acid (CGA) has been demonstrated to have a variety of physiological properties, including anti-inflammatory, antioxidant, immunomodulatory, anti-bacterial and antitumor effects ( Naveed et al, 2018 ), and it is widely recognized for its role in modulating skin function, possibly as a bioactive component of MQEF ( Lee et al, 2021 ). CGA photoprotection protects HDF cells from UVA-induced photoaging by reducing the accumulation of UVA-induced ROS, mitigating DNA damage, inhibiting the degradation of collagen and enhancing the synthesis of collagen ( Xue et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Modified Qing’e Formula protects against UV-induced skin oxidative damage via the activation of Nrf2/ARE defensive pathway

et al. 2022

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Exposure to ultraviolet (UV) light triggers the rapid generation and accumulation of reactive oxygen species (ROS) in skin cells, which increases oxidative stress damage and leads to photoaging. Nuclear factor E2-related factor 2 (Nrf2) modulates the antioxidant defense of skin cells against environmental factors, especially ultraviolet radiation. Natural products that target Nrf2-regulated antioxidant reactions are promising candidates for anti-photoaging. The aim of this study was to investigate the protective effect of Modified Qing’e Formula (MQEF) on UV-induced skin oxidative damage and its molecular mechanisms. In this study, the photoaging models of human keratinocytes (HaCaT) and ICR mice were established by UV irradiation. In vitro models showed that MQEF displayed potent antioxidant activity, significantly increased cell viability and reduced apoptosis and excess ROS levels. Meanwhile, the knockdown of Nrf2 reversed the antioxidant and anti-apoptotic effects of MQEF. In vivo experiments indicated that MQEF could protect the skin against UV-exposed injury which manifested by water loss, sensitivity, tanning, wrinkling, and breakage of collagen and elastic fibers. The application of MQEF effectively increased the activity of antioxidant enzymes and reduced the content of malondialdehyde (MDA) in mice. In addition, MQEF was able to activate Nrf2 nuclear translocation in mouse skin tissue. In summary, MQEF may attenuate UV-induced photoaging by upregulating Nrf2 expression and enhancing antioxidant damage capacity. MQEF may be a potential candidate to prevent UV-induced photoaging by restoring redox homeostasis.

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Impact of chlorogenic acid on modulation of significant genes in dermal fibroblasts and epidermal keratinocytes

Cited by 28 publications

References 24 publications

Assessment of Cosmetic and Dermatological Properties and Safety of Use of Model Skin Tonics with Kombucha-Fermented Red Berry Extracts

Assessment of Cosmetic and Dermatological Properties and Safety of Use of Model Skin Tonics with Kombucha-Fermented Red Berry Extracts

Trichosanthes kirilowii Extract Promotes Wound Healing through the Phosphorylation of ERK1/2 in Keratinocytes

Modified Qing’e Formula protects against UV-induced skin oxidative damage via the activation of Nrf2/ARE defensive pathway

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