Impact of citrate- and chitosan-capped gold nanoparticles on the liver of Swiss albino mice: Histological and cyto-genotoxic study

This study was to explore the mechanism of action of nanomaterial-loaded clarithromycin (CLA) after sinusitis surgery. Under the guidance of dynamic enhanced scanning (DES). 120 patients with sinusitis admitted to the First Affiliated Hospital of China Medical University from July 2019 to March 2020 were selected and divided into a control group and an observation group according to the random number table method, with 60 cases in each group. Then, the CLA-containing nano-polylactic acid material was prepared, observed with the scanning electron microscope (SEM), and its drug release ability was tested. All patients underwent endoscopic sinus surgery under general anesthesia. After the surgery was completed, patients in the control group were given only CLA capsules, and patients in the observation group were given freshly prepared nanomaterial-loaded CLA, and both groups of patients were continuously observed for two weeks. After that, the patients were examined using the dynamic enhancement computed tomography (CT). The clinical efficacy, serum interleukin-4 (IL-4), interleukin-8 (IL-8), and tumor necrosis factor α (TNF-α) levels of the two groups of patients were observed. The secretions of the patients' sinuses were performed with microbial bacterial culture, and the results were observed and recorded. Results showed that the characterization and analysis of the nano drug-carrying preparation suggested that the polylactic acid nanomembrane showed linear fiber morphology, relatively dense distribution, not greatly different fiber diameter, and small porosity. Characterization under a field of view (FOV) of 500 um showed that the fiber surface was smooth and rich in content. The release of CLA showed a gradual and steady upward trend. On the 25 th day, nearly 50% of the dose had been released, and it had reached more than 90% of the total release on the 55 th day. According to the statistics on the clinical efficacy of patients, it was found that the number of cured and effective patients in the observation group was higher than that of the control group, while the number of ineffective cases was much lower than that of the control group. The dynamic enhanced CT examination results of the patients in the control group after treatment showed that the soft tissue mass on the posterior right side of the nasopharynx was reduced, but the pharyngeal suture still existed; while those in the observation group showed that the plain scan density was uniform, and the mastoid air cells were clear on both sides. The number of cases with Staphylococcus aureus (S. aureus), Staphylococcus saprophyticus (S. saprophyticus), and Pasteurella multocida infections in the observation group were observably lower than those of the control group (P< 0.05), and it was the same case for the levels of serum IL-4, IL-8, and TNF-α. Conclusion: after dynamic enhanced CT scanning, it can be found that the nanomaterialloaded CLA increased the utilization rate of the drug, showing good clinical efficacy, and effectively improved the clinical sym...

Section: Level Of Il-4 Il-8 and Tnf-αmentioning

confidence: 99%

The Mechanism of Action of Nanomaterials Loaded with Clarithromycin after Sinusitis Surgery under the Guidance of Dynamic Enhanced Scanning

Ren¹,

Ma²,

Li³

et al. 2022

“…Ultrasound contrast agents present the advantages of high targeting, high efficiency, and miniaturization. It should be noted that by inorganic and organic nanomaterials, various medical applications are possible (27)(28)(29)(30). In this study, PLA-lecithin NMBs with good dispersibility were prepared.…”

Section: Comparison Of Adverse Reactions and Tumor Inhibition Rates B...mentioning

confidence: 99%

Preparation of Nano-microbubbles and Curative Effect of 125I Particles for Primary Liver Cancer Guided by Abdominal Interventional Ultrasound

Chen¹,

Gao²,

Yuan³

2022

This study aimed at the preparation of nano-microbubbles (NMBs) and the curative effect of 125 I particles for primary liver cancer guided by abdominal interventional ultrasound. NMBs were prepared by the thin-film hydration method, and NMBs and 125 I particles were connected by biotin to obtain the NMBs-125 I complex.36 patients with primary liver cancer treated in X Hospital from January to December 2019 were selected. NMBs-125 I particle implantation was guided by abdominal interventional ultrasound. Computed tomography (CT) or magnetic resonance imaging (MRI) examinations were performed 3 months after treatment, and patients were divided into remission and non-remission groups on account of the results. Contrast-enhanced ultrasound (CEUS) was performed before and 1 month after treatment to analyze and compare the changes in ultrasound parameters. It was shown that there was a good correlation 3 months after treatment between the maximum diameters of the tumor measured by CEUS and CT/MRI, respectively (r=0.856, P<0.01). In the remission group, tRTpre and tTTPprc 1 month after treatment were higher than those before treatment, but tPIpre and tSERmax.pre were lower than those before treatment. In the non-remission group, tRTpre and tTTPprc 1 month after treatment were lower than those before treatment, but tPIpre and tSERmax.pre were higher than those before treatment. Therefore, CEUS had a higher value on the efficacy of the NMBs-125 I particle implantation for primary liver cancer.

“…Blood samples were collected from all mice examined by the retro-orbital venous plexus. Sera were separated and stored at -80 o C until being used, as reported previously by Zaki et al (16). The serum concentration of testosterone was measured using the commercial testosterone immunoassay kit according to Amballi et al (17).…”

Section: Testosterone Hormone Measurementsmentioning

confidence: 99%

Cytogenetic and testicular histological alterations induced by sulphur dioxide in dried apricot leather

Zaki

El-Mahdy

EL-Gamal

et al. 2021

Self Cite

Sulphur dioxide (SO2) is used as a preservative in food to prevent its discolouration, and to inhibit the growth of bacteria. Little data is available concerning its in vivo hazardous impact.The present study is therefore designed to examine the cyto-genotoxic potential and the testicular histological alterations in adult mice, induced by SO2 present in the dried apricot leather used to prepare the oriental drink Qamar Al-Deen. Two different forms of drinks were tested; cold and boiled drinks. Animals were placed into 4 groups. The first group received distilled water as a negative control.The second and third groups received orally the drink for 28 days in the form of a cold and a boiled drink, respectively. Animals of the fourth group received cyclophosphamide, they were used as a positive control for cyto-genotoxic tests. The chromosomal aberrations, as well as sperm abnormalities, were significantly elevated in animals that received the two different drink preparations. The mitotic index significantly decreased in comparison with negative and positive controls. Furthermore, histological examination showed different degrees of alterations in the testis. Our results suggest that the presence of SO2 inside the apricot leather might be responsible for these changes. Thus, these remarkable hazardous effects of SO2 on male albino mice could be used as a potential guide for the prediction of its human health impact. Furthermore, consumers could be advised to prevent excessive consumption of the drink (Qamar Al-Deen) prepared from dried apricot leather.