Impact of confinement and food access restriction on the three-spined stickleback (Gasterosteus aculeatus, L.) during caging: a multi-biomarker approach

Catteau, Audrey; Guernic, Antoine Le; Marchand, Adrien; Hani, Younes Mohamed Ismail; Palluel, Olivier; Turiès, Cyril; Bado‐Nilles, Anne; Dedourge-Geffard, Odile; Geffard, Alain; Porcher, Jean‐Marc

doi:10.1007/s10695-019-00670-1

Cited by 16 publications

(6 citation statements)

References 81 publications

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“…This induction could mean a lost in the equilibrium of the metabolism in fish that normally compensates ROS generated by mitochondrial respiration through their antioxidant system. Then, overcrowding represents an additional stress factor which alters their homeostasis, as also Catteau et al () evidenced in Gasterosteus aculeatus exposed to confinement and starvation. Since the main role of GST is to catalyse the conjugation of glutathione (GSH) to electrophilic centres on a wide variety of compounds to make the substance more soluble, the energy demand to cope with overcrowding stress could interfere with the synthesis pathway (Biswas & Rahman, ).…”

Section: Discussionmentioning

confidence: 75%

“…Since the main role of GST is to catalyse the conjugation of glutathione (GSH) to electrophilic centres on a wide variety of compounds to make the substance more soluble, the energy demand to cope with overcrowding stress could interfere with the synthesis pathway (Biswas & Rahman, ). Then, fish ran out of energy to synthesize GSH and, consequently, to maintain GST normal activity (Catteau et al, ).…”

Section: Discussionmentioning

confidence: 99%

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Physiological effects of stocking density on the fishPiaractus mesopotamicusfed with red seaweed (Pyropia columbina) and β‐carotene‐supplemented diets

et al. 2020

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This study aimed at investigating the physiological responses of Piaractus mesopotamicus exposed to high stocking density and the potential protective role of supplemented diets. Fish were fed with basal, red seaweed (Pyropia columbina) or β-carotene-supplemented diets for 90 days. Then, fish were distributed at low (1.5 g/L) and high (22 g/L) stocking densities for 15 days. Fish exposed to the high density showed increased hepatosomatic index, haemoglobin content and mean corpuscular haemoglobin concentration (all diets); decreased haematocrit, mean corpuscular volume (basal and seaweed) and white blood cells count (all diets) were observed. High density-exposed fish showed decreased plasmatic metabolites as well as the hepatic lipids content in basal and seaweed diets. Regarding oxidative stress, increased activity of glutathione S-transferase in high density-exposed fish muscle (all diets), and lower lipid peroxidation in liver (basal and β-carotene) and intestine (basal and seaweed) were evidenced. Interactions between diet and stocking density were recorded regarding the triglycerides (decrease in fish exposed to high density fed with basal and seaweed) and hepatic lipids (decrease in fish exposed to high density fed with basal). The major changes occurred in haematologic and metabolic parameters as strategies to cope with overcrowding stress. Fish response to stocking density was not affected by diets. K E Y W O R D Saquaculture, crowding stress, dietary supplementation, energy reserves, haematology, oxidative stress | 1993

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Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Physiological effects of stocking density on the fishPiaractus mesopotamicusfed with red seaweed (Pyropia columbina) and β‐carotene‐supplemented diets

et al. 2020

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“…After 21 days of caging, as recommended [ 21 ] and already tested by Catteau et al [ 11 , 15 , 45 ], sticklebacks were directly anesthetised in situ by balneation with MS222 (tricaine methanesulfonate, 100 mg/L, Tricaine Pharmaq, Overhalla, Norway) to avoid handling stress and then killed by cervical dislocation. Each fish was weighed, measured and sexed and each organ was sampled for further analysis.…”

Section: Methodsmentioning

confidence: 99%

“…These biomarkers have proven their relevance for active biomonitoring [ 15 , 18 , 19 ]. The optimal conditions of stickleback caging have already been characterized to minimize the impact on biomarker responses [ 19 , 20 , 21 ]. However, the monitoring of genotoxicity at different scales in the frame of active methods has not been included in these multi-biomarker approaches in stickleback, although these effects are important to consider.…”

Section: Introductionmentioning

confidence: 99%

Integration of Genotoxic Biomarkers in Environmental Biomonitoring Analysis Using a Multi-Biomarker Approach in Three-Spined Stickleback (Gasterosteus aculeatus Linnaeus, 1758)

Cant

Bonnard

Porcher

et al. 2022

Toxics

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Water is impacted by a variety of increasing pressures, such as contaminants, including genotoxic pollutants. The proposed multi-biomarker approach at a sub-individual level gives a complementary indicator to the chemical and ecological parameters of the Water Framework Directive (WFD, 2000/60/EC). By integrating biomarkers of genotoxicity and erythrocyte necrosis in the sentinel fish species the three-spined stickleback (Gasterosteus aculeatus) through active biomonitoring of six stations of the Artois-Picardie watershed, north France, our work aimed to improve the already existing biomarker approach. Even if fish in all stations had high levels of DNA strand breaks, the multivariate analysis (PCA), followed by hierarchical agglomerative clustering (HAC), improved discrimination among stations by detecting an increase of nuclear DNA content variation (Etaing, St Rémy du Nord, Artres and Biache-St-Vaast) and erythrocyte necrosis (Etaing, St Rémy du Nord). The present work highlighted that the integration of these biomarkers of genotoxicity in a multi-biomarker approach is appropriate to expand physiological parameters which allow the targeting of new potential effects of contaminants.

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“…The caging was carried out in autumn, between September and December 2018. Autumn corresponds to the sexual rest period of G. aculeatus and D. polymorpha and therefore constitutes an appropriate season for the use of these models in biomonitoring to limit the variability linked to reproduction (Catteau et al, 2019;Palais et al, 2012). As far as practicable, all species have been caged in all sites studied.…”

Section: Experimental Designmentioning

confidence: 99%

Interest of a multispecies approach in active biomonitoring: Application in the Meuse watershed

Catteau

Porcher

Bado‐Nilles

et al. 2022

Science of The Total Environment

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Impact of confinement and food access restriction on the three-spined stickleback (Gasterosteus aculeatus, L.) during caging: a multi-biomarker approach

Cited by 16 publications

References 81 publications

Physiological effects of stocking density on the fishPiaractus mesopotamicusfed with red seaweed (Pyropia columbina) and β‐carotene‐supplemented diets

Physiological effects of stocking density on the fishPiaractus mesopotamicusfed with red seaweed (Pyropia columbina) and β‐carotene‐supplemented diets

Integration of Genotoxic Biomarkers in Environmental Biomonitoring Analysis Using a Multi-Biomarker Approach in Three-Spined Stickleback (Gasterosteus aculeatus Linnaeus, 1758)

Interest of a multispecies approach in active biomonitoring: Application in the Meuse watershed

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