This study investigated the association between both polymorphisms and
metabolic marker changes of the leptin gene, and body weight, weight gain,
carcass traits, feed intake, and feed conversion ratio. Blood samples were
collected from 60 V-line and 60 Baladi Black rabbits for DNA extraction and
biochemical analysis. DNA sequencing of leptin (202-bp) revealed four
non-synonymous single nucleotide polymorphism (SNPs) that characterized a
number of V-line rabbits. Statistical analysis revealed that the identified
SNPs were associated with rabbit growth and carcass measurements (p < 0.05).
A discriminant analysis model showed a high classification percentage for
the identified SNPs within and between breeds using body weight at 5-14
weeks of age (91.7%), slaughter traits (91.6%), feed intake, daily feed
intake, and feed conversion ratio (76.7%). This study reveals that leptin
gene could be a candidate for growth traits in rabbits enabling the
development of marker-assisted selection (MAS) in different rabbit breeds.