2006
DOI: 10.1002/mrd.20686
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Impact of cryopreservation and reactive oxygen species on DNA integrity, lipid peroxidation, and functional parameters in ram sperm

Abstract: Assisted reproduction using frozen-thawed semen has practical advantages, although cryopreservation is detrimental to sperm fertility in most mammals. We examined the influence of cryopreservation and reactive oxygen species (ROS) on ram sperm DNA stability (using SCSA), lipid peroxidation (LPO), chlortetracycline fluorescence (CTC) patterns, motility and viability. In Experiment 1, DNA integrity, LPO, CTC, motility and viability tests were performed on fresh and cryopreserved sperm after 0, 6, and 24 hr in sy… Show more

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Cited by 169 publications
(135 citation statements)
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“…This antioxidant capacity in sperm cells may, however, be insufficient in preventing LPO during the freeze-thawing process. In that regard, mammalian spermatozoa lack a substantial cytoplasmic component, which contains antioxidants that counteract the damaging effects of ROS and LPO (Peris et al 2007). Thiols such as GSH and GSSG are a large class of antioxidants.…”
mentioning
confidence: 99%
“…This antioxidant capacity in sperm cells may, however, be insufficient in preventing LPO during the freeze-thawing process. In that regard, mammalian spermatozoa lack a substantial cytoplasmic component, which contains antioxidants that counteract the damaging effects of ROS and LPO (Peris et al 2007). Thiols such as GSH and GSSG are a large class of antioxidants.…”
mentioning
confidence: 99%
“…Similarly, mechanical stress in other species as different as deer and dog does not lead to DNA damage [57,58], which suggests that mammalian sperm DNA can withstand some substantial amount of artificial physical stress and must also be very robust to any naturally occurring mechanical stress. By contrast, cryopreservation can lead to sperm DNA damage in some species such as ram, boar and alpaca [53,59,60], but not in others such as human, horse, bull and dog [58,[61][62][63].…”
Section: Discussionmentioning
confidence: 97%
“…The axosome and associated dense fibers of the middle pieces in sperm are covered by mitochondria that generate energy from intracellular stores of ATP. It is well established that ROS can induce axonemal and mitochondial damage, resulting in the immobilization of sperm (Aitken & Clarkson, 1987;Peris et al, 2007). Destruction of membrane integrity causes a raise in membrane permeability and decreases the ability of sperm to control the intracellular concentrations of ions involved in the sperm motion (Baumber et al, 2000).…”
Section: Discussionmentioning
confidence: 99%