Impact of dairy manure pre-application treatment on manure composition, soil dynamics of antibiotic resistance genes, and abundance of antibiotic-resistance genes on vegetables at harvest

Tien, Yuan-Ching; Li, Bing; Zhang, Tong; Scott, Andrew; Murray, Roger; Sabourin, Lyne; Marti, Romain; Topp, Edward

doi:10.1016/j.scitotenv.2016.12.138

Cited by 165 publications

(89 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Peak et al (2007) also observed that resistance gene levels are highly seasonal in wastewater lagoons with concentrations being 10-100 times greater in the autumn compared to the summer (Peak et al, 2007). With regard to the significant increase in relative abundance of erm(F) on the second farm, Tien et al (2017) reported that this resistance gene was significantly more abundant in anaerobically digested dairy manure than in the raw manure (Tien et al, 2017). This could be due to the fact that some microorganisms are enriched during waste treatment (Tien et al, 2017).…”

Section: Discussionmentioning

confidence: 98%

“…With regard to the significant increase in relative abundance of erm(F) on the second farm, Tien et al (2017) reported that this resistance gene was significantly more abundant in anaerobically digested dairy manure than in the raw manure (Tien et al, 2017). This could be due to the fact that some microorganisms are enriched during waste treatment (Tien et al, 2017). In light of the results from the present study, this suggests that some resistance genes might be more abundant after treatment of the of tet(B))) is presented for the different fractions sampled on both farms.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Presence and fate of antibiotic residues, antibiotic resistance genes and zoonotic bacteria during biological swine manure treatment

Meersche

Rasschaert

Haesebrouck

et al. 2019

Ecotoxicology and Environmental Safety

View full text Add to dashboard Cite

A B S T R A C TThe presence and dissemination of antibiotic residues, antibiotic resistance genes and zoonotic bacteria in the environment is of growing concern worldwide. Manure management practices, such as biological removal of nitrogen from swine manure, may help to decrease levels of antibiotic residues, antibiotic resistance genes and zoonotic bacteria present in manure before fertilization, thereby reducing environmental contamination. Therefore, the aim of this study was to monitor the presence and fate of seven antibiotic residues (colistin, sulfadiazine, trimethoprim, doxycycline, oxytetracycline, ceftiofur and tylosin A), nine antibiotic resistance genes (tet(B), tet(L), tet(M), tet(O), tet(Q), tet(W), erm(B), erm(F) and sul2) and two zoonotic bacteria (Salmonella Typhimurium and Campylobacter coli) during biological nitrogen removal from swine manure over time. Samples from the raw manure, the solid fraction, the liquid fraction and the storage lagoon were analyzed on two farms at six time points with an interval of two weeks. Only the antibiotics which were used during the three months preceding the first sampling could be detected before and after biological nitrogen removal from swine manure. Of all the antibiotics studied, doxycycline was recovered in all of the samples and sulfadiazine was recovered in most samples on both farms. For both antibiotics, there appears to be a reduction of the amount of residues present in the storage lagoon compared to the liquid fraction, however, this reduction was not statistically significant. A significant reduction of the relative abundances of most of the antibiotic resistance genes studied was observed when comparing the liquid fraction and the storage lagoon. For tet(L), no differences were observed between the fractions sampled and for sul2 and erm(F), a significant increase in relative abundances was observed on the second farm sampled. For the zoonotic bacteria, a reduction of at least 1 log was observed after biological nitrogen removal from swine manure. The results indicate that the concentration of certain antibiotic residues and several antibiotic resistance genes and the amount of zoonotic bacteria present in the manure may be reduced in the end product of the biological nitrogen removal from swine manure. Coördinatiecentrum Mestverwerking, 2017). For the biological removal https://doi.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Presence and fate of antibiotic residues, antibiotic resistance genes and zoonotic bacteria during biological swine manure treatment

Meersche

Rasschaert

Haesebrouck

et al. 2019

Ecotoxicology and Environmental Safety

View full text Add to dashboard Cite

show abstract

“…In some confined production practices, manure can be managed to reduce the burden of AMR prior to land application through digestion or composting (12). Fundamental and translational research in this domain has rich potential to develop and validate means of reducing agricultural antimicrobial use and thus human exposure to AMR throughout the food chain or the various environments impacted by agricultural waste.…”

Section: Risk Managementmentioning

confidence: 99%

Agriculture and Agri-Food Canada’s research program on antimicrobial resistance

Topp

2017

Canada Communicable Disease Report

View full text Add to dashboard Cite

A key strategy for attenuating the development of antimicrobial resistance (AMR) is ensuring judicious use of antimicrobials in human and veterinary medicine and in agriculture. Research on AMR in agriculture includes risk assessment, risk management, and identifying the role of agricultural practices in development of AMR. Risk assessment includes an impact assessment of antimicrobial use in livestock and on the environment; for example, many antimicrobials are excreted unchanged and thus reach the environment through manure application. This creates the potential for AMR transmission through the food processing chain and into agro-ecosystems receiving the agricultural waste. Risk management includes the assessment of cost-effective methods to keep animals healthy without the need for antimicrobial use, such as the use of vaccines, nutritional supplements and pre-, pro-or synbiotics and of waste management strategies to avoid AMR transmission. Currently, there is an important gap in understanding the degree of human exposure to AMR that is generated through agriculture, the burden of illness of AMR pathogens in human populations and the relationship between exposure and burden of illness. It is important that research on the agricultural, environmental and human medicine dimensions of AMR not be undertaken in silos, which is why the United Nations and countries around the world are working together within the One Health Framework that considers the inter-relatedness of humans, animals and the environment.

show abstract

“…To combat antibiotic resistance strategies-including reduced use of antibiotics in livestock and avoiding the use of clinically-important antibiotics for human medicine in livestock-should be complemented with manure management strategies (Meek et al, 2015). The potential for tailored manure management strategies to mitigate the spread of antibiotic resistance is gaining attention (Arikan et al, 2009;Tien et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Microbiota and Antibiotic Resistome of Lettuce Leaves and Radishes Grown in Soils Receiving Manure-Based Amendments Derived From Antibiotic-Treated Cows

Fogler

Guron

Wind

et al. 2019

Front. Sustain. Food Syst.

View full text Add to dashboard Cite

Cattle are commonly administered antibiotics, resulting in excretion of antibiotics, antibiotic-resistant bacteria (ARB), and antibiotic resistance genes (ARGs). The aim of this study was to determine if the use of dairy manure collected during antibiotic administration influences the bacterial microbiota of lettuce and radishes, including carriage of ARB and ARGs, when applied as a soil amendment and if composting mitigates the effects. Lettuce and radishes were grown in field-plots amended with raw manure from antibiotic-treated (cephapirin, pirlimycin) cows, composted manure from antibiotic-treated cows, composted manure from antibiotic-free cows, or an inorganic chemical fertilizer (control; 12 plots, n = 3). Surficial vegetable bacteria and antibiotic resistomes (i.e., total ARGs) were characterized using heterotrophic plate counts (HPCs) on antibiotic-containing media, 16S rRNA gene amplicon sequencing, quantitative polymerase chain reaction (qPCR), and shotgun metagenomics. The different manure and compost amendments did not result in significant changes to the surficial vegetable bacteria at the phylum level; however, some minor changes at the class and family level were observed. Beta-diversities of the ARGs detected by shotgun metagenomic sequencing were distinctly different between vegetable type (R = 0.30, p = 0.04), with small separations between the resistomes associated with amendment type in unrarefied analysis (R = 0.27, p = 0.02), but not rarefied analysis, of the data. Network analysis highlighted that multi-drug ARG classes commonly co-occurred with plasmid-associated genes and could be a driver of co-and cross-selection of ARGs in the different conditions. Carriage of sul1 and tet(W) ARGs on vegetables quantified by qPCR were strong indicators of manure-based amendment relative to chemical fertilizer, with some reduction incurred via composting (p < 0.05). Also, increased HPCs resistant/tolerant to clindamycin, a class of antibiotics administered to cattle, were on lettuce grown in biological soil amendments relative to chemical fertilizer (p < 0.05). This study demonstrates that amending soil with raw manure collected from dairy cows during Fogler et al. Biological Amendment Affects Vegetable Resistome antibiotic administration may affect the composition of microbiota and resistomes associated with vegetable surfaces. Composting may be an important strategy to reduce some ARGs on fresh produce, but differences in the resistomes of lettuce and radishes suggest the extent of soil contact should be considered.

show abstract

Impact of dairy manure pre-application treatment on manure composition, soil dynamics of antibiotic resistance genes, and abundance of antibiotic-resistance genes on vegetables at harvest

Cited by 165 publications

References 30 publications

Presence and fate of antibiotic residues, antibiotic resistance genes and zoonotic bacteria during biological swine manure treatment

Presence and fate of antibiotic residues, antibiotic resistance genes and zoonotic bacteria during biological swine manure treatment

Agriculture and Agri-Food Canada’s research program on antimicrobial resistance

Microbiota and Antibiotic Resistome of Lettuce Leaves and Radishes Grown in Soils Receiving Manure-Based Amendments Derived From Antibiotic-Treated Cows

Contact Info

Product

Resources

About