Impact of dietary ingredients on the interpretation of various fecal parameters in rats fed inulin

Chen, Hui-Ju; Dai, Fan-Jhen; Chang, Chih-Ren; Lau, Y. C.; Chew, Boon-Swee; Chau, Chi‐Fai

doi:10.1016/j.jfda.2019.06.005

Cited by 5 publications

(4 citation statements)

References 31 publications

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“…This could be due to the fact that acidification decreases the interactions between SCFAs and other substances present in the sample (Tomaino, Parker, & Larick, 2001). Previous studies using formic acid for SCFAs analyses were applied on the samples of feces (Chen et al., 2019; García‐Villalba et al., 2012; Liebisch et al., 2019; Zhao, Nyman, & Åke Jönsson, 2006) and bacteria (Wang et al., 2017). Our research is the first report to apply formic acid for SCFAs analyses on the plasma samples in which SCFAs are very important components for use as health indices.…”

Section: Resultsmentioning

confidence: 99%

“…The results of this study clearly showed that using formic acid at a concentration of 1.0 M led to the most efficient extraction. GC is employed as a highly applicable tool for analyzing SCFAs in samples such as human feces (Chen et al., 2019; Tangerman & Nagengast, 1996), colon and feces samples from rats and humans (Zhao et al., 2006), biogas digesters (Mulat & Feilberg, 2015), and others. GC detection with the FID (flame ionization detector) method is very sensitive for organic compound.…”

Section: Resultsmentioning

confidence: 99%

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Fast quantification of short‐chain fatty acids in rat plasma by gas chromatography

Wang

Chen

Tain

et al. 2020

Journal of Food Science

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Short‐chain fatty acids (SCFAs) are the main metabolites of the intestinal flora and play an important role in the interaction between the intestinal flora and host metabolism. Therefore, reliable methods are needed to accurately measure SCFAs concentrations. SCFAs are commonly analyzed by gas chromatography–mass spectrometry (GC‐MS), which requires lengthy sample treatments and a long run time. This study aimed to develop a fast GC method with formic acid pretreatment for SCFAs quantification in the plasma of rat. Baseline chromatographic resolution was achieved for three SCFAs (acetic, propionic, and butyric) within an analysis time of 10.5 min. The method exhibited good recovery for a wide range of concentrations with a low limit of detection for each compound. The relative standard deviations (RSDs) of all targeted compounds showed good intra‐ and interday precision (<10%). We used our method to measure SCFAs levels in plasma samples from rats fed with a high fructose diet (HFD) to test the accuracy of the developed method. It was shown that SCFAs are indeed affected negatively by a HFD (60% fructose). This method was successfully employed to accurately determine SCFAs in the rat plasma with minimum sample preparation. Results showed potential damage of HFD, which produced lower SCFAs. Practical Application Increasingly, microbiota and gut health research are being conducted by many food scientists to elucidate the relationships among the factors of food components, particularly the nondigestible carbohydrates, food processing conditions, and potential health impact. This research provides a useful, rapid, and accurate method that can save time in the analysis of short‐chain fatty acids, which are commonly analyzed in gut health research.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Fast quantification of short‐chain fatty acids in rat plasma by gas chromatography

Wang

Chen

Tain

et al. 2020

Journal of Food Science

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“…Low consumption of FODMAP and fecal microbiota transplantation improve symptoms and quality of life, restoring intestinal endocrine cell density and improving quality of life. There is evidence that patients who do not respond well to the diet may be offered fecal intestinal microbiota transplantation [44][45][46][47][48][49].…”

Section: The Relationship Between Diet Microbiota and Endocrine Cellsmentioning

confidence: 99%

Possibilities of Correction of Functional Disorders of the Gastrointestinal Tract in Patients with Anxiety Disorders

Volodikhina¹,

Akhmedova²,

Uzdenov³

et al. 2022

Pharmacophore

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Functional disorders of the gastrointestinal tract have recently occupied a niche of the most common diagnoses in gastroenterology, recognized by morphological and physiological abnormalities. Functional disorders of the gastrointestinal tract are a heterogeneous group of disorders, including not only symptoms characteristic of functional dyspepsia (14.6%) in combination with changes in intestinal motility but also irritable bowel syndrome (irritable bowel syndrome, 31.7%) and constipation. These are diseases classified by gastrointestinal manifestations, including one of the following symptoms: impaired motor skills, visceral hypersensitivity, changes in the function of the mucous membrane and the immune system, changes in the intestinal microbiota, and changes in the processing of the central nervous system. According to several studies, mental disorders such as depression and anxiety are often accompanied by functional disorders of the gastrointestinal tract. Thus, it can be concluded that functional disorders of the gastrointestinal tract require a multidisciplinary approach for timely diagnosis and treatment. A recent global Internet survey of the Rome Foundation, in which 127 54 people from 26 countries participated, reported that 32 112 (43%) of the respondents met the criteria for at least one of the manifestations of functional disorders of the gastrointestinal tract. Given the high prevalence of the disease, it is necessary to clearly understand the possibilities of correcting functional disorders of the gastrointestinal tract. This will allow researchers and practitioners to approach the treatment and prevention of this pathology more meaningfully.

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“…Results have reported that consuming enough inulin as a prebiotic may help maintain gut health, reduce the risk of infection, and enhance stool quality, along with improving microbiota, pH, short-chain fatty acids, and frequency. This inulin-type prebiotic's advantageous physiological effects also included control of obesity and diabetes as well as elevation of blood lipid levels and absorption of minerals in humans (Chen et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Impact of galacto-oligosaccharides on prebiotic potential in the intestinal microbiota fermentation and health status in an animal model

BAJWA,

IQBAL,

SOHAIB

et al. 2023

Food Sci. Technol (Campinas)

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The present study was conducted to assess selected galacto-oligosaccharides (GOS) effects on short-chain acids (SCFA), microbiota variability in in vitro, and health benefits using an animal model. In the in-vitro anaerobic batch, fermentation was applied to different groups divided by a varied amount of GOS sources, mixtures, and prebiotics. Results reported that SCFA for inulin contributed significantly higher for acetic, propionic, and butyric acids, and resistant starch (RS) showed a non-significant effect for acetic and propionic acids whereas the combined effect of GOS and RS showed higher values for parameters. For bacterial enumeration of bifidobacteria compared to individual GOS, synergistic effects were documented. The Sprague-Dawley rats given GOS under western diet influence relative to a high-fat diet alone observed after 1 and 4 weeks documented significant levels for acetic and butyric acid production, whereas body and organ weights for cecum tend to increase after 4 weeks of dietary intervention (p<0.05). Microbiome data using gene sequencing revealed a higher proportion of firmicutes and lower Bacteroides in control rats, which means Lachnospiraceae family abundances were higher in HF+GOS group. Overall, GOS fermentation showed an increment in the bifidobacterial population and tend to raise levels of SCFA in rats fed on a high-fat diet alone, whereas non-significant variation was reported in microbiome diversity after intervention. The present study was conducted to assess selected galacto-oligosaccharides (GOS) effects on short-chain acids (SCFA), microbiota variability in in vitro, and health benefits using an animal model. In the in-vitro anaerobic batch, fermentation was applied to different groups divided by a varied amount of GOS sources, mixtures, and prebiotics. Results reported that SCFA for inulin contributed significantly higher for acetic, propionic, and butyric acids, and resistant starch (RS) showed a non-significant effect for acetic and propionic acids whereas the combined effect of GOS and RS showed higher values for parameters. For bacterial enumeration of bifidobacteria compared to individual GOS, synergistic effects were documented. The Sprague-Dawley rats given GOS under western diet influence relative to a high-fat diet alone observed after 1 and 4 weeks documented significant levels for acetic and butyric acid production, whereas body and organ weights for cecum tend to increase after 4 weeks of dietary intervention (p<0.05). Microbiome data using gene sequencing revealed a higher proportion of firmicutes and lower Bacteroides in control rats, which means Lachnospiraceae family abundances were higher in HF+GOS group. Overall, GOS fermentation showed an increment in the bifidobacterial population and tend to raise levels of SCFA in rats fed on a high-fat diet alone, whereas non-significant variation was reported in microbiome diversity after intervention.

show abstract

Impact of dietary ingredients on the interpretation of various fecal parameters in rats fed inulin

Cited by 5 publications

References 31 publications

Fast quantification of short‐chain fatty acids in rat plasma by gas chromatography

Fast quantification of short‐chain fatty acids in rat plasma by gas chromatography

Possibilities of Correction of Functional Disorders of the Gastrointestinal Tract in Patients with Anxiety Disorders

Impact of galacto-oligosaccharides on prebiotic potential in the intestinal microbiota fermentation and health status in an animal model

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