Impact of dietary Mannan-oligosaccharide and β-Glucan supplementation on growth, histopathology, E-coli colonization and hepatic transcripts of TNF-α and NF- ϰB of broiler challenged with E. coli O78

Abstract

Background: Using probiotics have become popular. They are considered an alternative to Antibiotic Growth Promoters (AGP). Probiotics are supplemented into animal feed for improving growth performance along with preventing and controlling enteric pathogens. The aim of this work was to study the impact of dietary supplementation of Mannan-oligosaccharide and β-Glucan (Agrimos®) on broiler challenged with Escherichia coli O 78 (E. coli O 78-marked with an antibiotic (320 μg ciprofloxacin/ml broth) on growth performance, serum biochemistry, immune organs-histopathology, E-coli colonization, and hepatic transcripts of Tumor necrosis factoralpha (TNF-α) and Nuclear factor-kappa B (NF-ϰB). A total of 125 one-day-old chicks were used for conducting the experiment. Five one-day-old chicks were slaughtered for measuring the initial weight of the lymphoid tissue. The remaining chicks (120) were allotted into four groups according to Mannan-oligosaccharide and β-Glucan supplementation, and E. coli infection. The data were analyzed using SPSS version 16. Results: Results indicated significant alteration of growth performance, serum biochemistry, and selected liver gene expression with pathological lesions, especially in the lymphoid organs due to E. coli infection. These alterations were mitigated by Mannan-oligosaccharide and β-Glucan supplementation. Conclusion: It could be concluded, Mannan-oligosaccharide and β-Glucan supplementation in broiler's diet improved the immune response of broilers and mitigated pathological lesion resulted from E. coli infection.

Ameliorative effect of nanocurcumin and Saccharomyces cell wall alone and in combination against aflatoxicosis in broilers

“…As shown in our study, the addition of Saccharomyces cell wall/Nanocurcumin alone/in combination to the aflatoxin-contaminated diet alleviated the toxic effects of aflatoxin B1 on the hepatic function in broilers fed on for 35 days, where the serum total protein, albumin, and globulin concentrations were increased. These results are compatible with previous studies [ 63 – 65 ]. On the other hand, Li et al [ 66 ] explained that YCW incorporation did not affect the same biochemical indices, including total protein, albumin, and globulin.…”

Dietary hydrolyzed wheat gluten supplementation ameliorated intestinal barrier dysfunctions of broilers challenged with Escherichia coli O78

“…In general, pathogenic E. coli -infection is associated with reduced feed intake and intestinal mucosal damage ( Mellata, 2013 ). In the present study, we found that E. coli O78 infection resulted in increased immune-related organs indexes (spleen index and thymus index) and impaired gut mucosal morphology of broilers, indicating that animal model of E. coli infection has been successfully established ( Fadl et al, 2020 ). Previous studies have demonstrated that functional amino acids, like Gln and tryptophan ( Trp ), exerted positive role in anti-inflammation and cell proliferation, which might account for the amelioration of immune organic compensatory hypertrophy and the intestinal mucosal impairment of E. coli O78-induced broilers ( Maiorka et al, 2016 ; Wang et al, 2020 ).…”

Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers

“…The probiotic dietary inclusion decreased the serum activities of ALT, AST, and ALP in the laying hen ( 62 ). Other findings reported that probiotics dietary supplementation without heat stress does not affect the serum activities of AST and ALT ( 66 ). These results have been confirmed by the results of MDA and antioxidant enzymes.…”