Impact of Environmental Factors on Secondary Metabolite Diversity and Free Radical Scavenging Activity of V .vinefera Jumbo Seedless From Nashik Valley

Abstract

An environmental factor has great influence on biosynthesis of secondary metabolites in plants. Nashik valley is located at highest elevated point of the Maharashtra state of India. Thereby, vineyards of these areas are always subjected to various stressful environmental conditions. Present work aimed to study the effect of environmental factors on secondary metabolite diversity and free radical scavenging activity of different aerial parts of V.Vinefera Nashik valley. Organic extracts are prepared by soaking the dry powder (10%) of different aerial parts viz.leaf lamina, stem and petiole of Jumbo seedless cultivar into 90% Methanol. Presence of different secondary metabolites was confirmed by phytochemical analysis. Detection of spots of secondary metabolites was observed by Thin layer chromatography (TLC) using the solvent system, N Butanol: Acetic Acid: Water (4:1:5). Folin method was used to determine total phenolic content whereas, total flavonoid content was estimated by aluminum chloride colorimetric assay. Free radical scavenging activity of extract was carried out by DPPH assay .Findings of study showed that almost all the aerial parts of V.vinefera are rich source of secondary metabolites with medicinal values. Results of TLC showed presence of different spots of secondary metabolites. Rf value of flavonoid quercetin was found to be 0.85. Total phenolic content of petiole was found to be highest (0.92± 0.10)