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V. vinifera is one of the important fruit crop, characterized by substantial number of soluble flavonoids including quercetin, the clinically proved therapeutic agent. Present study has been focused on analysis of flavonoids together with quercetin in aerial parts of V.vinifera and their correlation with radical scavenging activity (RSA). Two varieties of black cultivars viz. Sharad and Sonaka seedless were selected. Pr esence of flavonoid was confirmed by phytochemical analysis. Bands of flavonoids were detected by Preparative thin layer chromatography (PTLC). Total flavonoid content (TFC) and radical scavenging activity (RSA) was estimated by AlCl3 colorimetric assay and DPPH assay respectively. Quantification of quercetin was done by HPLC. Findings of analysis showed high abundance of flavonoids in almost all parts of both cultivars. Rf value for quercetin was found to be 0.85. Aerial parts of both cultivars showed significant amount of TFC. Petiole of Sonaka seedless showed highest content of flavonoid (0.28 ± 0.06 mg/g QE). Whereas, stem of Sharad seedless showed less content (0.10 ± 0.02 mg/g QE). Concentration of quercetin in berry skin of Sharad seedless was found to be 2133.4 ppm. Order of RSA for both the cultivars was Petiole>Stem>Leaf lamina. In Sharad seedless less significant correlation was found between TFC and RSA. Whereas positive correlation was shown by Sonaka seedless Therefore, it can be con cluded that in addition to skin, almost all the aerial parts of V.vinifera are rich source of flavonoids with noteworthy RSA i.e antioxidant potential. Thus, significant amount of flavonoids can be efficiently extracted from these aerial parts of V.vinifera which can be used for healing of various diseases and disorders

An environmental factor has great influence on biosynthesis of secondary metabolites in plants. Nashik valley is located at highest elevated point of the Maharashtra state of India. Thereby, vineyards of these areas are always subjected to various stressful environmental conditions. Present work aimed to study the effect of environmental factors on secondary metabolite diversity and free radical scavenging activity of different aerial parts of V.Vinefera Nashik valley. Organic extracts are prepared by soaking the dry powder (10%) of different aerial parts viz.leaf lamina, stem and petiole of Jumbo seedless cultivar into 90% Methanol. Presence of different secondary metabolites was confirmed by phytochemical analysis. Detection of spots of secondary metabolites was observed by Thin layer chromatography (TLC) using the solvent system, N Butanol: Acetic Acid: Water (4:1:5). Folin method was used to determine total phenolic content whereas, total flavonoid content was estimated by aluminum chloride colorimetric assay. Free radical scavenging activity of extract was carried out by DPPH assay .Findings of study showed that almost all the aerial parts of V.vinefera are rich source of secondary metabolites with medicinal values. Results of TLC showed presence of different spots of secondary metabolites. Rf value of flavonoid quercetin was found to be 0.85. Total phenolic content of petiole was found to be highest (0.92± 0.10)