Impact of exogenous GABA treatments on endogenous GABA metabolism in anthurium cut flowers in response to postharvest chilling temperature

Aghdam, Morteza Soleimani; Naderi, Roohangiz; Jannatizadeh, Abbasali; Babalar, Mesbah; Sarcheshmeh, Mohammad Ali Askari; Faradonbe, Mojtaba Zamani

doi:10.1016/j.plaphy.2016.04.045

Cited by 50 publications

(21 citation statements)

References 25 publications

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“…During chilling stress, ROS production has been shown to irreversibly damage chloroplasts, mitochondria and apoplast, among other organelles and macromolecules . H 2 O 2 can also increase oxidative damage and peroxidation of membrane lipids by the generation of the hydroxyl radicals . In this experiment, treating fruit with 60 and 90 μmol L −1 melatonin resulted in significantly lower H 2 O 2 levels after 20 days of storage compared to control fruit (Fig.…”

Section: Resultsmentioning

confidence: 66%

“…These results suggest that the decrease in CI following melatonin treatment in sapota fruit may be associated with the higher GABA and proline content. GABA has been shown to accumulate in plants under stress and involved in osmotic regulation, reactive oxygen radical detoxication, conversion of putrescine to proline and intracellular signal transduction . Increased postharvest chilling tolerance in horticultural commodities in relation to GABA has been attributed to the accumulation of higher proline levels, which in turn is related to higher pyrroline‐5‐carboxylate synthetase and ornithine aminotransferase enzyme activities, which coincide with lower proline dehydrogenase enzyme activity …”

Section: Resultsmentioning

confidence: 99%

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Postharvest melatonin treatment reduces chilling injury in sapota fruit

et al. 2020

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BACKGROUND Sapota is a popular tropical fruit characterized by a very short postharvest life. Low‐temperature storage prolongs postharvest life of sapota fruit, but chilling injury symptoms can develop if the storage temperature is less than 14 °C. There have been no reports on the effects of postharvest melatonin application on the development of chilling injury in sapota fruit during cold storage. The objective of this study was to investigate the effects of different concentrations of postharvest melatonin application (0, 30, 60 and 90 μmol L−1) during cold storage (8 °C) for up to 30 days with an additional 1‐day shelf life at ambient temperature. RESULTS All melatonin treatments reduced chilling injury symptoms, reduced electrolyte leakage, malondialdehyde (MDA) content, H2O2 and superoxide anion (O2−), and increased proline content and the activity of superoxide dismutase (SOD), catalase (CAT) and γ‐aminobutyric acid (GABA), and reduced the activities of phospholipase D (PLD) and lipoxygenase (LOX) compared to the control. CONCLUSION Postharvest melatonin treatment could be a useful strategy for reducing chilling injury during cold storage and transport of sapota fruit. The results indicate that melatonin reduces chilling injury of sapota fruit through maintaining membrane integrity, SOD and CAT activities, and reducing PLD and LOX activities. © 2019 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 66%

Section: Resultsmentioning

confidence: 99%

Postharvest melatonin treatment reduces chilling injury in sapota fruit

et al. 2020

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“…The accumulation of ROS in fruit with CI damage during long‐term storage is a consequence of an imbalance between the generation and deduction of ROS, which leads to damage to the cellular components and cellular membranes . One of the proposed mechanisms, but not the only one, by which exogenous GABA alleviates CI might be through maintaining energy status by providing nicotinamide adenine dinucleotide (NAD) + H+(NADH), adenosine triphosphate (ATP), and the inhibition of cytoplasm acidification, as well as by endogenous GABA accumulation during chilling stress . Reduction of CI is accompanied with the activity of the antioxidant enzymes under cold storage conditions to scavenge ROS, which could be another mechanism of action.…”

Section: Discussionmentioning

confidence: 99%

Postharvest treatments with γ‐aminobutyric acid, methyl jasmonate, or methyl salicylate enhance chilling tolerance of blood orange fruit at prolonged cold storage

et al. 2019

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BACKGROUND Blood orange is sensitive to chilling injury (CI) depending on cultivar and storage temperature. Postharvest treatments with γ‐aminobutyric acid (GABA), methyl jasmonate (MeJA), or methyl salicylate (MeSA) are known to alleviate CI. γ‐Aminobutyric acid aqueous solution, applied at 20 and 40 mM, was vacuum‐infiltrated at 30 kPa for 8 min at 20 °C. Methyl jasmonate or MeSA vapor treatments were applied separately at 50 and 100 μM by putting the fruit in 20 L plastic containers for 18 h at 20 °C. There have been no reports about postharvest treatments of GABA, MeJA, or MeSA on enhancing the tolerance of ‘Moro’ blood orange to chilling during long‐term cold storage at 3 °C for 150 days, which was the subject of this study. RESULTS All treatments significantly alleviated CI symptoms of blood orange manifested by lower electrolyte leakage (EL), malondialdehyde (MDA), hydrogen peroxide (H2O2) concentrations, and higher proline content in flavedo during storage. The largest effects were obtained with 100, 50 μM, and 40 mM for MeSA, MeJA, and GABA, respectively, which enhanced the activity of the antioxidant enzymes catalase (CAT), ascorbate peroxidase (APX) and superoxide dismutase (SOD), and phenylalanine ammonia‐lyase (PAL). On the other hand, these treatments suppressed peroxidase (POD) and polyphenol oxidase (PPO) activities. CONCLUSION The mechanisms involved in enhancing the tolerance of ‘Moro’ blood orange to chilling could involve scavenging H2O2 by increasing the activity of antioxidant enzymes, higher PAL/PPO activity ratio, and osmoregulation by increasing proline content. These changes led to the maintenance of the epidermis structure. This was confirmed by scanning electron microscopy (SEM) micrographs. © 2019 Society of Chemical Industry

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“…In plants, GABA plays roles in diverse processes including the carbon:nitrogen balance, signaling, regulation of redox status, development, stress responses (Batushansky et al, 2014; Batushansky et al, 2015; Michaeli & Fromm, 2015; Molina-Rueda et al, 2015; Shi et al, 2010; Yu et al, 2014). Recent studies have indicated that GABA is required for proper growth during exposure to abiotic stresses such as salt (Renault et al, 2013), drought (Mekonnen, Flügge & Ludewig, 2016), low temperature (Aghdam et al, 2016), Zn 2+ (Daş et al, 2016), and cadmium (Sun et al, 2010). Additionally, exogenous GABA has been shown to regulate the expression of genes such as BnNrt2 in Brassica napus (Beuve et al, 2004), 14-3-3 in Arabidopsis thaliana (Lancien & Roberts, 2006), SAMDC (S-adenosylmethionine decarboxylase) in Cucumis melo (Wang et al, 2014), and to control gene transcription in Caragana intermedia (Shi et al, 2010) and A. thaliana (Batushansky et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Identification of two CiGADs from Caragana intermedia and their transcriptional responses to abiotic stresses and exogenous abscisic acid

Zheng

Yue

et al. 2017

PeerJ

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BackgroundGlutamate decarboxylase (GAD), as a key enzyme in the γ -aminobutyric acid (GABA) shunt, catalyzes the decarboxylation of L-glutamate to form GABA. This pathway has attracted much interest because of its roles in carbon and nitrogen metabolism, stress responses, and signaling in higher plants. The aim of this study was to isolate and characterize genes encoding GADs from Caragana intermedia, an important nitrogen-fixing leguminous shrub.MethodsTwo full-length cDNAs encoding GADs (designated as CiGAD1 and CiGAD2) were isolated and characterized. Multiple alignment and phylogenetic analyses were conducted to evaluate their structures and identities to each other and to homologs in other plants. Tissue expression analyses were conducted to evaluate their transcriptional responses to stress (NaCl, ZnSO4, CdCl2, high/low temperature, and dehydration) and exogenous abscisic acid.ResultsThe CiGADs contained the conserved PLP domain and calmodulin (CaM)-binding domain in the C-terminal region. The phylogenetic analysis showed that they were more closely related to the GADs of soybean, another legume, than to GADs of other model plants. According to Southern blotting analysis, CiGAD1 had one copy and CiGAD2-related genes were present as two copies in C. intermedia. In the tissue expression analyses, there were much higher transcript levels of CiGAD2 than CiGAD1 in bark, suggesting that CiGAD2 might play a role in secondary growth of woody plants. Several stress treatments (NaCl, ZnSO4, CdCl2, high/low temperature, and dehydration) significantly increased the transcript levels of both CiGADs, except for CiGAD2 under Cd stress. The CiGAD1 transcript levels strongly increased in response to Zn stress (74.3-fold increase in roots) and heat stress (218.1-fold increase in leaves). The transcript levels of both CiGADs significantly increased as GABA accumulated during a 24-h salt treatment. Abscisic acid was involved in regulating the expression of these two CiGADs under salt stress.DiscussionThis study showed that two CiGADs cloned from C. intermedia are closely related to homologs in another legume, soybean. CiGAD2 expression was much higher than that of CiGAD1 in bark, indicating that CiGAD2 might participate in the process of secondary growth in woody plants. Multiple stresses, interestingly, showed that Zn and heat stresses had the strongest effects on CiGAD1 expression, suggesting that CiGAD1 plays important roles in the responses to Zn and heat stresses. Additionally, these two genes might be involved in ABA dependent pathway during stress. This result provides important information about the role of GADs in woody plants’ responses to environmental stresses.

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Impact of exogenous GABA treatments on endogenous GABA metabolism in anthurium cut flowers in response to postharvest chilling temperature

Cited by 50 publications

References 25 publications

Postharvest melatonin treatment reduces chilling injury in sapota fruit

Postharvest melatonin treatment reduces chilling injury in sapota fruit

Postharvest treatments with γ‐aminobutyric acid, methyl jasmonate, or methyl salicylate enhance chilling tolerance of blood orange fruit at prolonged cold storage

Identification of two CiGADs from Caragana intermedia and their transcriptional responses to abiotic stresses and exogenous abscisic acid

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