Impact of Exopolysaccharides (EPSs) of Lactobacillus gasseri strains isolated from human vagina on cervical tumor cells (HeLa)

Sungur, Tolga; Aslım, Belma; Karaaslan, Çağatay; Aktaş, Büşra

doi:10.1016/j.anaerobe.2017.05.013

Cited by 110 publications

(64 citation statements)

References 48 publications

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“…Our analysis revealed that DSPS is a heteropolysaccharide comprising six monosaccharides. EPSs produced by endophytes are strain‐dependent, and their structural and compositional diversity conveys unique and varied biological activities . Given that endophytes can be grown in large‐scale cultures, have a short growth period, and are easily isolated, the 14‐DS‐1 strain isolated in this study may have great potential for future applications.…”

Section: Discussionmentioning

confidence: 99%

“…A growing body of evidence has revealed that microbial EPSs are beneficial to human health; they show promising activities, including immunomodulation and cytotoxic effects against cancer cells . Importantly, emerging studies have revealed that the structural units of the EPS not only determine its function, but the ecological niches of the host microorganism also contribute to EPS activity . For example, EPS produced by Paenibacilluspolymyxa , a bacterium isolated from Stemona japonica (Blume) Miquel , exhibited robust scavenging activities for superoxide and hydroxyl radicals .…”

Section: Introductionmentioning

confidence: 99%

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Exopolysaccharides from a Codonopsis pilosula endophyte activate macrophages and inhibit cancer cell proliferation and migration

Chen

et al. 2018

Thoracic Cancer

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BackgroundExopolysaccharides with structural diversity have shown wide applications in biomaterial, food, and pharmaceutical industries. Herein, we isolated an endophytic strain, 14‐DS‐1, from the traditional medicinal plant Codonopsis pilosula to elucidate the characteristics and anti‐cancer activities of purified exopolysaccharides.MethodsHPLC and GC‐MS were conducted to purify and characterize the exopolysaccharides isolated from 14‐DS‐1. Quantitative RT‐PCR, cell migration assays, immunofluorescence staining, and flow cytometry analysis were conducted to investighate the biological activity of DSPS.ResultsWe demonstrated that exopolysaccharides isolated from 14‐DS‐1 (DSPS), which were predominately composed of six monosaccharides, showed anti‐cancer activities. Biological activity analysis revealed that exposure to DSPS induced macrophage activation and polarization by promoting the production of TNF‐α and nitric oxide. Further analysis revealed that DSPS treatment promoted macrophage infiltration, whereas cancer cell migration was suppressed. In addition, DSPS exposure led to S‐phase arrest and apoptosis in cancer cells. Immunofluorescence staining revealed that treatment with DSPS resulted in defects in spindle orientation and positioning.ConclusionThese findings thus suggest that DSPS may have promising potential in cancer therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exopolysaccharides from a Codonopsis pilosula endophyte activate macrophages and inhibit cancer cell proliferation and migration

Chen

et al. 2018

Thoracic Cancer

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“…Moreover, EPS from L. acidophilus inhibited the expressions of genes involved in tumor angiogenesis and survival of the colon cancer cell lines in vitro (106). Similarly, EPS from L. acidophilus LA1 demonstrated their anti-tumor activity in vivo against Ehrlich ascites carcinoma cells by suppressing the serum levels of malondialdehyde and nitric oxide (116) and EPS of Lactobacillus gasseri strains also showed their capability to inhibit cervical cancer cell growth and modulate immune response (117).…”

Section: Cell-wall Polysaccharidesmentioning

confidence: 98%

Paraprobiotics and Postbiotics of Probiotic Lactobacilli, Their Positive Effects on the Host and Action Mechanisms: A Review

et al. 2020

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Lactobacilli comprise an important group of probiotics for both human and animals. The emerging concern regarding safety problems associated with live microbial cells is enhancing the interest in using cell components and metabolites derived from probiotic strains. Here, we define cell structural components and metabolites of probiotic bacteria as paraprobiotics and postbiotics, respectively. Paraprobiotics and postbiotics produced from Lactobacilli consist of a wide range of molecules including peptidoglycans, surface proteins, cell wall polysaccharides, secreted proteins, bacteriocins, and organic acids, which mediate positive effect on the host, such as immunomodulatory, anti-tumor, antimicrobial, and barrier-preservation effects. In this review, we systematically summarize the paraprobiotics and postbiotics derived from Lactobacilli and their beneficial functions. We also discuss the mechanisms underlying their beneficial effects on the host, and their interaction with the host cells. This review may boost our understanding on the benefits and molecular mechanisms associated with paraprobiotics and probiotics from Lactobacilli, which may promote their applications in humans and animals.

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“…Many enzymes and peptides and the lactic acid secreted by Lactobacillus are involved in activating and regulating important signaling molecules and pathways in cervical cancer. 43 , 44 It is necessary to elucidate the specific molecular mechanism or construct the molecular regulatory network in future research. Lactobacillus acts as a valuable cloning vector and is currently mainly used as a plasmid vector for the experimental research of cancer in vitro.…”

Section: Future Directionsmentioning

confidence: 99%

Role of <em>Lactobacillus</em> in cervical cancer

Yang¹,

Da²,

Zhang³

et al. 2018

CMAR

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Cervical cancer is a common malignant cancer among women worldwide. Changes in the vaginal microecological environment lead to multiple gynecological diseases, including cervical cancer. Recent research has shown that Lactobacillus may play an important role in the occurrence and development of cervical cancer. This review explores the role of Lactobacillus in cervical cancer. A total of 29 articles were included after identification and screening. The pertinent literature on Lactobacillus in cervical cancer from two perspectives, including clinical studies and experimental studies, was analyzed. An association network for the mechanism by which Lactobacillus induces cervical cancer was constructed. In addition, we provide direction and insight for further research on the role of Lactobacillus in cervical cancer.

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Impact of Exopolysaccharides (EPSs) of Lactobacillus gasseri strains isolated from human vagina on cervical tumor cells (HeLa)

Cited by 110 publications

References 48 publications

Exopolysaccharides from a Codonopsis pilosula endophyte activate macrophages and inhibit cancer cell proliferation and migration

Exopolysaccharides from a Codonopsis pilosula endophyte activate macrophages and inhibit cancer cell proliferation and migration

Paraprobiotics and Postbiotics of Probiotic Lactobacilli, Their Positive Effects on the Host and Action Mechanisms: A Review

Role of <em>Lactobacillus</em> in cervical cancer

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