Impact of fluoride and a static magnetic field on the gene expression that is associated with the antioxidant defense system of human fibroblasts

Kimsa-Dudek, Magdalena; Synowiec-Wojtarowicz, Agnieszka; Derewniuk, Małgorzata; Gawron, S.; Paul-Samojedny, Monika; Kruszniewska‐Rajs, Celina; Pawłowska-Góral, Katarzyna

doi:10.1016/j.cbi.2018.04.004

Cited by 18 publications

(18 citation statements)

References 34 publications

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“…In addition, in a study of men, Sirmatel et al [10] found a decrease in the oxidative stress index (OSI) and total oxidant status (TOS) after exposure to a SMF. A similar SMF effect was also observed at the cellular level [11,12].…”

Section: Introductionsupporting

confidence: 77%

“…The NHDF cell line was purchased from Clonetics (CC-2511, San Diego, CA, USA) and was cultured as was previously described [11].…”

Section: Cell Culturementioning

confidence: 99%

“…For the experiment, the fibroblasts were plated at 5 × 10 5 cells in 25 cm 2 culture vessels (Sarstedt, Nümbrecht, Germany) and pre-incubated for 24 h in an atmosphere of 5% CO 2 at 37 • C. Then, the cells were treated with sodium fluoride (NaF) at a concentration of 0.3 mmol/L and were exposed to a moderate-strength SMF with different magnetic inductions (0.45 T; 0.55 T and 0.65 T) for 12 h. The NaF was dissolved in phosphate-buffered saline (PBS, Lonza, Basel, Switzerland), and a dilution was prepared in the culture medium. The concentration of fluoride was selected on the basis of previous research [11]. The SMF was generated using permanent magnets in patented magnetic chambers that were designed to test the impact of SMF in vitro (patent P-396639).…”

Section: Cell Culturementioning

confidence: 99%

“…The research was conducted with magnetic field intensities that were parametrically changed by altering the distance from the permanent magnets. In addition, a control was used in which steel was employed instead of a magnet (0.0 T) [11,12,19,20]. A Gauss meter was used to assess the flux densities.…”

Section: Cell Culturementioning

confidence: 99%

“…Total RNA was isolated using a TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. Qualitative and quantitative evaluations of the extracts were carried out as was previously described [11].…”

Section: Rna Extractionmentioning

confidence: 99%

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The Protective Effect of Static Magnetic Fields with Different Magnetic Inductions against Fluoride Toxicity Is Related to the NRF2 Signaling Pathway

2020

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A redox imbalance disrupts the cellcycle and the proliferation process, and contributes to the initiation of programmed cell death. One of the pathways that are important for redox homeostasis is the Nrf2-ARE signaling pathway. Fluoride as well as static magnetic fields (SMF) are associated with the concepts of oxidative stress, and thus programmed cell death. Therefore, this study aimed to assess the connection between oxidative stress and apoptosis in human cells co-exposed to fluoride and a SMF with a different magnetic induction and to determine whether the Nrf2-signaling pathway is involved in these effects. The research was realized using normal human dermal fibroblasts that had been co-exposed to fluoride (0.3 mmol/L) and a SMF with a different magnetic induction (0.45 T, 0.55 T, 0.65 T) for 12 h. The mRNA levels of the cellular antioxidant system-related genes and apoptosis-related genes were assessed using the quantitative reverse transcription polymerase chain reaction (RT-qPCR) method. Our results indicated that the increased activity of antioxidant enzymes (SOD1 (superoxide dismutase 1), SOD2 and GSR (glutathione reductase)) suggests the restoration of the cell redox homeostasis that had been disturbed by fluoride, and also that the genes whose expression is associated with the induction of apoptosis are down regulated as a result of exposure to a SMF. The SMF with a 0.65 T flux density had the strongest effect on the fibroblasts. Moreover, our findings demonstrated that the Nrf2 transcription factor plays a crucial role in the protective effect of a SMF against fluoride toxicity in human cells. The results of these studies can form the basis for developing therapeutic strategies for apoptosis and oxidative stress-related diseases.

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Section: Introductionsupporting

confidence: 77%

“…The NHDF cell line was purchased from Clonetics (CC-2511, San Diego, CA, USA) and was cultured as was previously described [11].…”

Section: Cell Culturementioning

confidence: 99%

Section: Cell Culturementioning

confidence: 99%

Section: Cell Culturementioning

confidence: 99%

Section: Rna Extractionmentioning

confidence: 99%

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The Protective Effect of Static Magnetic Fields with Different Magnetic Inductions against Fluoride Toxicity Is Related to the NRF2 Signaling Pathway

2020

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Impact of silver ions and silver nanoparticles on biochemical parameters and antioxidant enzyme modulations in Saccharomyces cerevisiae under co-exposure to static magnetic field: a comparative investigation

Kthiri,

Hamimed,

Tahri

et al. 2023

Int Microbiol

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The effect of a static magnetic field and baicalin or baicalein interactions on amelanotic melanoma cell cultures (C32)

et al. 2022

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Background Baicalin and baicalein have antioxidant, anti-inflammatory, hepatoprotective and anti-cancer properties. However, it is not known how a static magnetic field will modify these properties. Therefore, the aim of our study was to evaluate the simultaneous exposure of melanoma cells to flavones and the static magnetic fields that are generated by permanent magnets on the gene expression and the activity of the antioxidant enzymes that are associated with the antioxidant defense system. Methods and results Melanoma cells that had been treated with baicalin or baicalein were subjected to a static magnetic fields with a moderate induction. The static magnetic field was emitted by permanent magnets and the cell cultures were carried out in special test chambers. The research included determining the activity of the antioxidant enzymes (superoxide dismutase, glutathione peroxidase and catalase) as well as the gene expression profile. The addition of the flavones to the cell cultures at a concentration of 50 µmol/L resulted increase in the expression of the SOD1, SOD2 and GPX1 genes compared to the nontreated cell cultures. Simultaneous exposure of the melanoma cells to static magnetic field and baicalin or baicalein reduced their mRNA levels compared to the cultures to which only baicalin or baicalein had been added. The change in gene expression was accompanied by changes at the protein level associated with an increase in the activity of antioxidant enzymes. Conclusion We showed that baicalin or baicalein have anticancer properties by disturbing the redox homeostasis in melanoma cells and also increases the antioxidant system gene expression. There was also an antagonistic interaction between the studied flavones and the static magnetic field, which cause a decrease in the anticancer effects of baicalin or baicalein.

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Impact of fluoride and a static magnetic field on the gene expression that is associated with the antioxidant defense system of human fibroblasts

Cited by 18 publications

References 34 publications

The Protective Effect of Static Magnetic Fields with Different Magnetic Inductions against Fluoride Toxicity Is Related to the NRF2 Signaling Pathway

The Protective Effect of Static Magnetic Fields with Different Magnetic Inductions against Fluoride Toxicity Is Related to the NRF2 Signaling Pathway

Impact of silver ions and silver nanoparticles on biochemical parameters and antioxidant enzyme modulations in Saccharomyces cerevisiae under co-exposure to static magnetic field: a comparative investigation

The effect of a static magnetic field and baicalin or baicalein interactions on amelanotic melanoma cell cultures (C32)

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