Impact of formulation excipients on the thermal, mechanical, and electrokinetic properties of hydroxypropyl methylcellulose acetate succinate (HPMCAS)

Deshpande, Tanvi M.; Quadir, Anisul; Obara, Sakaé; Hoag, Stephen W.

doi:10.1016/j.ijpharm.2018.02.024

Cited by 13 publications

(4 citation statements)

References 28 publications

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“…These plasticizing polymers normally generate cracks as a dry final product after coating by fluidized bed. For this reason, coating preparation was improved with triethyl citrate, which gave greater firmness and remarkedly decreased cracks, effect that is corroborated with photomicrographs of the SEM (Deshpande et al, 2018). The influence of compounds that improve enteric coating based on compounds derived from hydroxypropyl methylcellulose (HPMC) is not yet clear.…”

Section: In Vitro Release Profilementioning

confidence: 93%

Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive

Roque‐Borda

Silva

Crusca

et al. 2021

International Journal of Biological Macromolecules

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Section: In Vitro Release Profilementioning

confidence: 93%

Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive

Roque‐Borda

Silva

Crusca

et al. 2021

International Journal of Biological Macromolecules

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“…Therefore, this study provides evidence for the potential use of the Ctx(Ile 21 )-Ha antimicrobial peptide to reduce S. Enteritidis counts in a chicken infection model. One of the most important aspects related to the successful application of this HPMCAS against S. Enteritidis is most likely the high mechanical resistance 52 and insolubility in the gastric pH of the molecule, which could guarantee the full release of the Ctx(Ile 21 )-Ha peptide. 29 In addition, the introduction of the peptide in alginate-based microparticles would allow its mucoadhesion, easy dispersion, and controlled release for a longer period and would protect the particles from denaturing agents.…”

Section: ■ Discussionmentioning

confidence: 99%

HPMCAS-Coated Alginate Microparticles Loaded with Ctx(Ile²¹)-Ha as a Promising Antimicrobial Agent against Salmonella Enteritidis in a Chicken Infection Model

et al. 2022

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Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) in poultry is most often transmitted by the fecal− oral route, which can be attributed to high population density. Upon encountering the innate immune response in a host, the pathogen triggers a stress response and virulence factors to help it survive in the host. The aim of this study was to evaluate the effect of hypromellose acetate/succinate (HPMCAS)-coated alginate microparticles containing the Ctx(Ile 21 )-Ha antimicrobial peptide (AMP) on both intestinal colonization and systemic infection of laying hens challenged with S. Enteritidis. The applied AMP microsystem reduced the bacterial load of S. Enteritidis in the liver, with a statistical significance between groups A (control, no Ctx(Ile 21 )-Ha peptide) and B (2.5 mg of Ctx(Ile 21 )-Ha/kg) at 2 days postinfection (dpi), potentially indicating the effectiveness of Ctx(Ile 21 )-Ha in the first stage of infection by S. Enteritidis. In addition, the results showed a significant decrease in the S. Enteritidis counts in the spleen and cecal content at 5 dpi; remarkably, no S. Enteritidis counts were observed in livers at 5, 7, and 14 dpi, regardless of the Ctx(Ile 21 )-Ha dosage (p-value <0.0001). Using the Chi-square test, the effect of AMP microparticles on S. Enteritidis fecal excretion was also evaluated, and a significantly lower bacterial excretion was observed over 21 days in groups B and C, in comparison with the untreated control (p-value <0.05). In summary, the use of HPMCAS-Ctx(Ile 21 )-Ha peptide microcapsules in laying hens drastically reduced the systemic infection of S. Enteritidis, mainly in the liver, indicating a potential for application as a feed additive against this pathogen.

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“…One of the most important aspects related to the successful application of this HPMCAS against S . Enteritidis, is most likely regarding to the high mechanical resistance 42 and insolubility in gastric pH of the molecule, which could guarantee the fully release of Ctx(Ile 21 )-Ha peptide 15 .…”

Section: Discussionmentioning

confidence: 99%

Application of HPMCAS-coated Ctx(Ile²¹)-Ha peptide microparticles as a potential use to prevent systemic infection caused bySalmonellaEnteritidis in poultry

Roque‐Borda

Saraiva

Monte

et al. 2021

Preprint

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The transmission of Salmonella Enteritidis (SE) in poultry is most often by the fecal-oral route, which can be attributed to the population density. Consequently, the pathogen triggers stress response and virulence factors deploying it to survive in hosts. Therefore, this study proposed to evaluate HPMCAS-coated microparticles containing the Ctx(Ile21)-Ha antimicrobial peptide against SE in laying hens chicks infection model to determine whether Ctx(Ile21)-Ha-utilization confers a benefit in the intestinal lumen, as well as whether limits systemic infection. Importantly, while assessing whether AMP utilization confers reduction of SE in liver, it was noted that there was statistical significance between groups A (control, no Ctx(Ile21)-Ha peptide) and B (2.5 mg of Ctx(Ile21)-Ha/kg) at 2 dpi, potentially indicating the Ctx(Ile21)-Ha effectiveness in the first stage of infection by SE. Remarkably, it was also detected a statistical significance (p -value <0.0001) with lower counts of SE (~ 0 CFU) in livers at 5, 7, and 14 dpi, regardless of Ctx(Ile21)-Ha dosage (2.5 mg or 5 mg/kg - group C). By using Chi-square test, the AMP effect on SE fecal excretion was evaluated. In this regard, it was noticed statistical significance (p < 0.05) among groups B and C in comparison with control group A, since those groups had lower bacterial excretion along 21 days. In summary, the role of HPMCAS-Ctx(Ile21)-Ha peptide microcapsules against S. Enteritidis in laying hen chicks infection model was unraveled, providing a satisfactory results against this pathogen.

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Impact of formulation excipients on the thermal, mechanical, and electrokinetic properties of hydroxypropyl methylcellulose acetate succinate (HPMCAS)

Cited by 13 publications

References 28 publications

Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive

Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive

HPMCAS-Coated Alginate Microparticles Loaded with Ctx(Ile²¹)-Ha as a Promising Antimicrobial Agent against Salmonella Enteritidis in a Chicken Infection Model

Application of HPMCAS-coated Ctx(Ile²¹)-Ha peptide microparticles as a potential use to prevent systemic infection caused bySalmonellaEnteritidis in poultry

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Impact of formulation excipients on the thermal, mechanical, and electrokinetic properties of hydroxypropyl methylcellulose acetate succinate (HPMCAS)

Cited by 13 publications

References 28 publications

Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive

Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive

HPMCAS-Coated Alginate Microparticles Loaded with Ctx(Ile21)-Ha as a Promising Antimicrobial Agent against Salmonella Enteritidis in a Chicken Infection Model

Application of HPMCAS-coated Ctx(Ile21)-Ha peptide microparticles as a potential use to prevent systemic infection caused bySalmonellaEnteritidis in poultry

Contact Info

Product

Resources

About

HPMCAS-Coated Alginate Microparticles Loaded with Ctx(Ile²¹)-Ha as a Promising Antimicrobial Agent against Salmonella Enteritidis in a Chicken Infection Model

Application of HPMCAS-coated Ctx(Ile²¹)-Ha peptide microparticles as a potential use to prevent systemic infection caused bySalmonellaEnteritidis in poultry