Impact of Green Extraction on Curcuminoid Content, Antioxidant Activities and Anti-Cancer Efficiency (In Vitro) from Turmeric Rhizomes (Curcuma longa L.)

Abstract

Turmeric (Curcuma longa L.) powder is widely used as a spice and seasoning in Asian countries. This study investigated the effect of turmeric extracts on the anticancer activity of Huh7 and HCT 116 cells. The curcumin bioactive compounds were extracted using various methods such as microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE) and traditional extraction (TDE). The yield of dried extracts from MAE was found to be the highest at 17.89%, followed by UAE and TDE, with 11.34% and 5.54%, respectively. Antioxidant activities such as TPC, DPPH and FRAP from MAE were higher than those of UAE and TDE. The total curcuminoid contents from the novel extractions were higher than those from traditional extraction methods. For instance, curcuminoid contents from MAE, UAE and TDE were 326.79, 241.17 and 215.83 mg/g, respectively. Due to having the highest bioactive compounds and extraction yield, turmeric extract from MAE was used to investigate the potential anticancer properties. The extract showed significant cytotoxic potential against the human liver (Huh7) and human colon (HCT116) cell lines, in concentrations ranging from 31.25 to 1000.00 µg/mL. Turmeric extracts using MAE have potential anticancer effects on Huh7 and HCT116 cells. This study serves as scientific data for the chemotherapeutic properties of turmeric extracts and their use as functional ingredients.

Oxidative Stress, Inflammation, Gut Dysbiosis: What Can Polyphenols Do in Inflammatory Bowel Disease?

“…Curcumin, a widely applied natural polyphenol mainly derived from CHMs (e.g., Crocus sativus L., and Curcuma longa L.) [ 81 , 82 ], has been reported to have promising pharmacologic activities in IBD therapy [ 83 ]. The antioxidant, anti-inflammatory, and antimicrobial properties of curcumin endow it with an enormous advantage in modulating the gut microbiota and alleviating oxidative stress and inflammation, thus protecting the intestinal mucosal barrier.…”

Development, characterization, and consumer acceptance evaluation of thermally stable capsule beads containing mixed extracts of green tea and turmeric

“…The rhizomes was ground by a hammer mill machine and passed through a 1.0 mm sieve. We utilized the extraction process protocol described in our previous study 24 . Brie y, 5 g of turmeric powder was extracted in 95% (v/v) ethanol (100 mL) using microwave-assisted extraction at 800 W for 3 minutes.…”

“…A standard solution of curcuminoid was prepared by mixing curcumin (80%), DMC (17%) and BDMC (3%) (Sigma, St. Louis, MI, USA). The HPLC protocol used to determine the curcuminoid content was implemented in this work 24 .…”

Xây Dựng Quy Trình Chiết Cao Chuẩn Hóa Kiểm Soát Hàm Lượng Curcuminoid Từ Thân Rễ Nghệ Vàng (Rhizoma Curcumae Longa)

“…Như vậy có thể thấy, sản lượng chiết xuất của chúng tôi khá cao. Nếu so sánh với các phương pháp chiết xuất công nghiệp khác, sản lượng chiết xuất khô thấp hơn đáng kể, dao động từ 5-18% [8]. Tuy nhiên, trong nghiên cứu của Đoàn Xuân Tuyền và cộng sự, tác giả cũng sử dụng cách thức chiết xuất tương tự chúng tôi, tổng hàm lượng phần trăm curcuminoid ghi nhận được hơn 50% [1].…”