Impact of Hepatic Arterial Reconstruction on Orthotopic Liver Transplantation in the Rat

Hori, Tomohide; Gardner, Lindsay B.; Chen, Florence; Baine, Ann Marie T.; Hata, Toshiyuki; Üemoto, Shinji; Nguyen, Justin H.

doi:10.3109/08941939.2011.636476

Cited by 14 publications

(13 citation statements)

References 42 publications

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“…Drug-induced DNA damage is not identified by the TUNEL assay unless it is coupled to the apoptotic response (34). In addition, this technique can detect early-stage apoptosis in systems where chromatin condensation has begun and strand breaks are fewer, even before the nucleus undergoes major morphological changes (34,35).…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical investigation of the coma blister and its pathogenesis

et al. 2013

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: The erythematous patches and vesicles that are observed in coma patients, usually from an overdose of medication, are known as coma blisters. However, it is unknown whether the degenerated sweat gland is a necrosis or apoptosis. We immunohistochemically examined such skin lesions to investigate the characteristics and pathogenesis of the coma blister. Skin lesions were obtained from a forensic autopsy case, a woman in her thirties, of caffeine intoxication. Those lesions were observed in the left femoral, the lower left thigh, and the right knee. Histologically, the skin lesions showed that the keratinocytes had necrosed and the epidermis was thin in some areas. Eccrine sweat gland degeneration was observed. Obvious inflammatory cell infiltrations were not detected. Immunohistochemically, we stained each skin lesion against CD3, CD8, CD45RO, cytokeratin, 70 kD heat shock protein, ubiquitin, 150 kD oxygen regulated protein, and caspase-cleaved keratin 18 neo-epitope M30. They were also stained with an in situ apoptosis detection kit. Degenerated sweat glands featured CD45RO and M30 immunoreactivity. Immunohistochemical staining for CD45RO, CK-L, and M30 might be useful to observe sweat gland degeneration in the coma blister. Therefore, the apoptosis might be related to coma blisters and sweat gland degenerations. J. Med. Invest. 60 : 256-261, August, 2013

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Section: Discussionmentioning

confidence: 99%

Immunohistochemical investigation of the coma blister and its pathogenesis

et al. 2013

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“…Orthotopic liver transplantation was performed using the “two-cuff technique” as previously described [11, 18]. Briefly, the donors and recipients were anesthetized with the ether.…”

Section: Methodsmentioning

confidence: 99%

Gene Silencing of 4-1BB by RNA Interference Inhibits Acute Rejection in Rats with Liver Transplantation

Shi

Song

et al. 2013

BioMed Research International

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The 4-1BB signal pathway plays a key role in organ transplantation tolerance. In this study, we have investigated the effect of gene silencing of 4-1BB by RNA interference (RNAi) on the acute rejection in rats with liver transplantation. The recombination vector of lentivirus that contains shRNA targeting the 4-1BB gene (LV-sh4-1BB) was constructed. The liver transplantation was performed using the two-cuff technique. Brown-Norway (BN) recipient rats were infected by the recombinant LVs. The results showed that gene silencing of 4-1BB by RNAi downregulated the 4-1BB gene expression of the splenic lymphocytes in vitro, and the splenic lymphocytes isolated from the rats with liver transplantation. LV-sh4-1BB decreased the plasma levels of liver injury markers including AST, ALT, and BIL and also decreased the level of plasma IL-2 and IFN-γ in recipient rats with liver transplantation. Lentivirus-mediated delivery of shRNA targeting 4-1BB gene prolonged the survival time of recipient and alleviated the injury of liver morphology in recipient rats with liver transplantation. In conclusion, our results demonstrate that gene silencing of 4-1BB by RNA interference inhibits the acute rejection in rats with liver transplantation.

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“…It has been amply demonstrated that - in contrast to the human situation - OLT and P-OLT in rats can be successfully performed without the technically complicated rearterialization of the graft [3,9,15,94]; nonetheless, the arterial flow has essential functions, and omitting rearterialization can substantially influence bile duct circulation (and thereby the rate of biliary complications) [18,95,96], graft microcirculation [97], and immunological functions [98]. This was also confirmed during our own investigations: we observed considerable differences in hepatocellular injury (AST and ALT levels) and graft microcirculation with and without arterial reconstruction [unpubl.…”

Section: Technical Issues Concerning P-olt In Ratsmentioning

confidence: 99%

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Czigány

Iwasaki²,

Yagi³

et al. 2015

Eur Surg Res

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Background: Due to a worldwide shortage of donor organs for liver transplantation, alternative approaches, such as split and living donor liver transplantations, were introduced to increase the donor pool and reduce mortality on liver transplant waiting lists. Numerous details concerning the mechanisms and pathophysiology of liver regeneration, small-for-size syndrome, rejection, and tolerance in partial liver transplantation facilitated the development of various animal models. The high number of preclinical animal studies contributed enormously to our understanding of many clinical aspects of living donor and partial liver transplantations. Summary: Microsurgical rat models of partial orthotopic liver transplantation are well established and widely used. Nevertheless, several issues regarding this procedure are controversial, not clarified, or not yet properly standardized (graft rearterialization, size reduction techniques, etc.). The major aim of this literature review is to give the reader a current overview of rat orthotopic liver transplantation models with a special focus on partial liver transplantation. The aspects of model evolution, microsurgical training, and different technical problems are analyzed and discussed in detail. Our further aim in this paper is to elaborate a detailed publication guide in order to improve the quality of reporting in the field of rat liver transplantation according to the ARRIVE guidelines and the 3R principle. Key Messages: Partial orthotopic liver transplantation in rats is an indispensable, reliable, and cost-efficient model for transplantation research. A certain consensus on different technical issues and a significant improvement in scientific reporting are essential to improve transparency and comparability in this field as well as to foster refinement.

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Impact of Hepatic Arterial Reconstruction on Orthotopic Liver Transplantation in the Rat

Cited by 14 publications

References 42 publications

Immunohistochemical investigation of the coma blister and its pathogenesis

Immunohistochemical investigation of the coma blister and its pathogenesis

Gene Silencing of 4-1BB by RNA Interference Inhibits Acute Rejection in Rats with Liver Transplantation

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

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