Impact of Histochemistry on biomedical research: looking through the articles published in a long-established histochemical journal

Pellicciari, C.

doi:10.4081/ejh.2014.2474

Cited by 4 publications

(2 citation statements)

References 107 publications

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“…Histochemistry provides a unique opportunity to document in situ the behavior of single cells in a complex organ [ 24 ]. The purpose of the present work was to document, mainly with histochemical procedures, the progression of oxidative stress in the liver of rats induced by a MCD diet lasting for 1 to 4 weeks, taking as reference the liver of rats submitted to a control diet, isocaloric with MCD diet, but containing methionine and choline.…”

Section: Introductionmentioning

confidence: 99%

In Situ Evaluation of Oxidative Stress in Rat Fatty Liver Induced by a Methionine‐ and Choline‐Deficient Diet

Freitas

Boncompagni

Tarantola

et al. 2016

Oxidative Medicine and Cellular Longevity

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Nonalcoholic fatty liver disease (NAFLD) is a serious health problem in developed countries. We documented the effects of feeding with a NAFLD-inducing, methionine- and choline-deficient (MCD) diet, for 1–4 weeks on rat liver oxidative stress, with respect to a control diet. Glycogen, neutral lipids, ROS, peroxidated proteins, and SOD2 were investigated using histochemical procedures; ATP, GSH, and TBARS concentrations were investigated by biochemical dosages, and SOD2 expression was investigated by Western Blotting. In the 4-week-diet period, glycogen stores decreased whereas lipid droplets, ROS, and peroxidated proteins expression (especially around lipid droplets of hepatocytes) increased. SOD2 immunostaining decreased in poorly steatotic hepatocytes but increased in the thin cytoplasm of macrosteatotic cells; a trend towards a quantitative decrease of SOD expression in homogenates occurred after 3 weeks. ATP and GSH values were significantly lower for rats fed with the MCD diet with respect to the controls. An increase of TBARS in the last period of the diet is in keeping with the high ROS production and low antioxidant defense; these TBARS may promote protein peroxidation around lipid droplets. Since these proteins play key roles in lipid mobilization, storage, and metabolism, this last information appears significant, as it points towards a previously misconsidered target of NAFLD-associated oxidative stress that might be responsible for lipid dysfunction.

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Section: Introductionmentioning

confidence: 99%

In Situ Evaluation of Oxidative Stress in Rat Fatty Liver Induced by a Methionine‐ and Choline‐Deficient Diet

Freitas

Boncompagni

Tarantola

et al. 2016

Oxidative Medicine and Cellular Longevity

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“…56,57 This approach would especially be convenient for studying the effects of nanovector-based therapies aimed at curing diseased cells which are to be preserved and functionally repaired (e.g. highly differentiated non-cycling cells such as myofibres and neurons, in muscular or neurodegenerative diseases); indeed, ultrastructural cytochemistry enables to finely describe the cellular morphofunctional changes induced by drug-delivering nanovectors, providing evidence for the restored healthy features and for the possible occurrence of undesired negative side-effects.…”

Section: Discussionmentioning

confidence: 99%

Transmission electron microscopy for nanomedicine: novel applications for long-established techniques

Malatesta

2016

Eur J Histochem

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During the last twenty years, the research in nanoscience and nanotechnology has dramatically increased and, in the last decade, the interest has progressively been oriented towards biomedical applications, giving rise to a new field termed nanomedicine. Transmission electron microscopy is a valuable technique not only for the thorough physico-chemical characterization of newly synthesized nanoparticulates, but especially to explore the effects of nanocomposites on biological systems, providing essential information for the development of efficient therapeutic and diagnostic strategies. Thus, for the progress of nanotechnology in the biomedical field, experts in cell biology, histochemistry and ultramicroscopy should always support the chemists, physicists and pharmacologists engaged in the synthesis and characterization of innovative nanoconstructs.

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Dentin matrix protein 1 and dentin sialophosphoprotein in human sound and carious teeth: an immunohistochemical and colorimetric assay

Martini

Trirè²,

Breschi

et al. 2013

Eur J Histochem

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Dentin matrix protein 1 (DMP1) and dentin sialophosphoprotein (DSPP) are extracellular matrix proteins produced by odontoblasts involved in the dentin mineralization. The aim this study was to compare the distribution of DMP1 and DSPP in human sound dentin vs human sclerotic dentin. Sixteen sound and sixteen carious human molars were selected, fixed in paraformaldehyde and processed for immunohistochemical detection of DMP1 and DSPP by means of light microscopy, transmission electron microscopy (TEM) and high-resolution field emission in-lens scanning electron microscopy (FEI-SEM). Specimens were submitted to a pre-embedding or a post-embedding immunolabeling technique using primary antibodies anti DMP1 and anti-DSPP and gold-conjugated secondary antibodies. Other samples were processed for the detection of DMP1 and DSPP levels. Dentin from these samples was mechanically fractured to powder, then a protein extraction and a protein level detection assay were performed. DMP1 and DSPP were more abundant in carious than in sound samples. Immunohistochemical analyses in sclerotic dentin disclosed a high expression of DMP1 and DSPP inside the tubules, suggesting an active biomineralization of dentin by odontoblasts. Furthermore, the detection of small amounts of these proteins inside the tubules far from the carious lesion, as shown in the present study, is consistent with the hypothesis of a preventive defense of all dentin after a noxious stimulus has undermined the tooth.

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Impact of Histochemistry on biomedical research: looking through the articles published in a long-established histochemical journal

Cited by 4 publications

References 107 publications

In Situ Evaluation of Oxidative Stress in Rat Fatty Liver Induced by a Methionine‐ and Choline‐Deficient Diet

In Situ Evaluation of Oxidative Stress in Rat Fatty Liver Induced by a Methionine‐ and Choline‐Deficient Diet

Transmission electron microscopy for nanomedicine: novel applications for long-established techniques

Dentin matrix protein 1 and dentin sialophosphoprotein in human sound and carious teeth: an immunohistochemical and colorimetric assay

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