Impact of HIV‐Positive Sera on the Functional Activity of Polymorphonuclear Neutrophils from Healthy Donors

Gabrilovich, Dmitry I.; Avdeeva, L. A.; Serebrovskaya, L. V.; Shepeleva, G. K.; Suvorova, Z. K.; Buravcova, E. V.; Oganezov, V. K.; Platonov, Alexander E.; Habarova, V. V.; Posly, I. M.; Pokrovsky, Vadim

doi:10.1111/j.1365-3083.1993.tb01752.x

Cited by 4 publications

(1 citation statement)

References 11 publications

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“…Phagocytic function has been reported as subnormal [17], supranormal [18], oxidative function as excessive [19] and impaired [20], and bacterial killing as reduced [21] and unchanged [22]. Two studies have suggested that heat-labile soluble serum factors may play an important part in HIV-associated neutrophil dysfunction [23,24]. The one functional test which appears to be impaired in all studies is chemotaxis.…”

Section: Discussionmentioning

confidence: 99%

Neutrophil adhesion molecules in HIV disease

Moore

Henderson

Gazzard

1998

Clinical and Experimental Immunology

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Neutrophil dysfunction in HIV disease is well described. We examined the expression of neutrophil adhesion molecules amongst 72 HIV‐infected subjects using a whole blood flow cytometric assay with FITC‐ and R‐PE‐labelled isotype‐specific MoAbs. We report lesser expression of CD11a (LFA‐1) and L‐selectin (CD62L) on the circulating neutrophils of HIV+ subjects compared with HIV− controls. Expression of CD11b (Mac‐1) was unchanged. Shedding of L‐selectin and up‐regulation of CD11b in response to in vitro stimulation with N‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP) were less in HIV+ compared with HIV− subjects, most markedly in subjects with CD4 cell counts < 100 cells/mm3. These results suggest that neutrophil dysfunction in HIV disease, which increases with disease progression, may be attributable to dysregulated adhesion molecule expression.

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Section: Discussionmentioning

confidence: 99%

Neutrophil adhesion molecules in HIV disease

Moore

Henderson

Gazzard

1998

Clinical and Experimental Immunology

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Modification of Lymphocyte and Monocyte Functional Activity by Polymorphonuclear Neutrophils in HIV Infection

et al. 1993

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The aim of this study was to determine whether polymorphonuclear neutrophils (PMN) can modify the immune response in HIV cases. Supernatants of PMN (PMNS) from 33 HIV-infected patients (16 with lymphoadenopathy syndrome, 17 with AIDS-related complex) were tested for their influence on the functional activity of lymphocytes and monocytes from 6 healthy donors. PMNS from another 6 healthy donors comprised a control group. It was found that PMNS from HIV-infected patients, but not from healthy donors, induced suppression of lymphocyte proliferative response and down-regulation of CD8 receptor expression on lymphocytes. Decrease of NK-cell cytotoxicity in the presence of PMNS from HIV-infected patients was the same as that from healthy donors. PMNS did not influence the production of anti-HIV antibody by lymphocytes from HIV-infected patients, as well as non-specific IgG by lymphocytes from healthy donors. PMNS effect on functional activity of lymphocytes was blocked completely after treatment of PMN by catalase and superoxide dismutase. At the same time PMNS from HIV-infected patients but not from healthy donors induced increased production of TNF-alpha by monocytes and up-regulation of monocyte phagocytosis. These effects were independent of catalase and superoxide dismutase and were not abrogated by antibody against IL-1, IL-8, TNF-alpha, IFN-gamma or IFN-alpha.

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The Human Innunodeficiency Virus Type I Tatt Protein Potentiates Ethanol‐Induced Neutrophil Functional Impairment in Transgenic Mice

Prakash

Zhang

Xie

et al. 1998

Alcoholism Clin & Exp Res

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Neutropenia and impairment of neutrophil function are commonly observed in patients with human immunodeficiency virus (HIV) infections. The HIV regulatory protein Tat is known to exert immunosuppressive effects. Alcohol is known also to be an immunosuppressive factor, and as alcohol abuse is common among HIV infected hosts, both factors may interact in an additive or synergistic fashion to further impair the host defenses of these patients. In order to test this possibility, endotoxin-induced neutrophil beta2-integrins CD11b and CD18 expression, phagocytosis, and hydrogen peroxide generation were examined in normal and HIV-1 Tat-transgenic mice in the absence and presence of ethanol intoxication. Acute ethanol intoxication was induced in mice (body weight of 25+/-1 g) by an intraperitoneal (ip) injection of ethanol (3 g/kg, 20% in normal saline). Thirty min later, the animals were given an ip injection of endotoxin (20 microg in 0.2 ml of saline/mouse). Vehicle-treated controls received an ip injection of saline without ethanol or endotoxin. Two hr after endotoxin administration, the animals were killed to determine neutrophil functions with flow cytometry. The baseline expression of CD11b and CD18 was similar in normal nontransgenic and Tat-transgenic mice. Endotoxin administration significantly up-regulated CD11b and CD18 expression in normal mice. This up-regulation was suppressed in Tat-transgenic mice. Ethanol intoxication inhibited endotoxin-induced CD11b and CD18 expression in normal mice and totally abolished endotoxin-induced CD11b and CD18 expression in Tat-transgenic mice. Neutrophil phagocytic activity in normal and Tat-transgenic mice was similar. Ethanol intoxication produced a similar inhibition of phagocytosis in both study groups. Endotoxin suppressed phagocytosis in normal mice, and this suppression was more pronounced in Tat-transgenic mice. Spontaneous and phorbol myristate acetate (PMA)-stimulated hydrogen peroxide generation by circulating neutrophils (PMNs) were similar in normal and Tat-transgenic mice. Neither ethanol nor endotoxin affected hydrogen peroxide generation by PMNs. These data show that both Tat and alcohol significantly impair PMN function, and this may be a mechanism leading to the increased susceptibility of the HIV-infected host to bacterial infection.

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Impact of HIV‐Positive Sera on the Functional Activity of Polymorphonuclear Neutrophils from Healthy Donors

Cited by 4 publications

References 11 publications

Neutrophil adhesion molecules in HIV disease

Neutrophil adhesion molecules in HIV disease

Modification of Lymphocyte and Monocyte Functional Activity by Polymorphonuclear Neutrophils in HIV Infection

The Human Innunodeficiency Virus Type I Tatt Protein Potentiates Ethanol‐Induced Neutrophil Functional Impairment in Transgenic Mice

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