Impact of human behavior on the spread of African swine fever virus: what every veterinarian should know

Rademacher, Christopher; Brown, Justin T.; Karriker, Locke A.

doi:10.2460/javma.22.06.0250

Cited by 4 publications

(3 citation statements)

References 24 publications

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“…In this regard, the portability of a LAMP PCR machine for fluorometric detection or the austere setup with which the colorimetric LAMP can be performed, would suggest that these tools can be applied for surveillance even in remote regions or backyard farms. This is important, considering that, as high as 98% of the outbreaks have been related to backyard productions in the Dominican Republic (Gonzales et al, 2021;Rademacher et al, 2022). In addition, wildlife plays a key role in the epidemiology of ASFV and must be taken into account in any surveillance program (Mighell and Ward, 2021;Sauter-Louis et al, 2021a;Urbano and Ferreira, 2022).…”

Section: Discussionmentioning

confidence: 99%

Efficient detection of African Swine Fever Virus using minimal equipment through a LAMP PCR method

Bohórquez

Lanka²,

Rosell³

et al. 2023

Front. Cell. Infect. Microbiol.

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African swine fever virus (ASFV) currently represents the biggest threat to the porcine industry worldwide, with high economic impact and severe animal health and welfare concerns. Outbreaks have occurred in Europe and Asia since ASFV was reintroduced into the continent in 2007 and, in 2021, ASFV was detected in the Caribbean, raising alarm about the reemergence of the virus in the Americas. Given the lack of vaccines against ASFV, control of the virus relies on molecular surveillance, which can be delayed due to the need for sample shipment to specialized laboratories. Isothermal PCR techniques, such as LAMP, have become increasingly attractive as point-of-care diagnostic tools given the minimal material expense, equipment, and training required. The present study aimed to develop a LAMP assay for the detection of ASFV. Four LAMP primer sets were designed, based on a consensus sequence for the ASFV p72 gene, and were tested using a synthetic plasmid containing the cloned ASFV p72 target gene as a positive control. Two primer sets, were selected for further validation, given their very short time for amplification. Both primer sets showed thermal stability, amplifying the ASFV DNA at temperatures between 60-70°C and proved to have an analytical limit of detection as low as one ASFV-plasmid DNA copy/µL, using both fluorometric and colorimetric methods. The selected primers did not yield false positive or cross reactive results with other common swine pathogens, showing high specificity. Testing of DNA-spiked samples showed that LAMP amplification was not affected by the nature of the matrices, including oral fluids, tonsils, blood, or rectal swabs. The primer sets were able to detect the two more prevalent ASFV genotypes in the field. Taken together, the results show that ASFV-LAMP-BG2 and ASFV-LAMP-BG3 would be a useful tool for rapid, highly sensitive on-site diagnostic testing.

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Section: Discussionmentioning

confidence: 99%

Efficient detection of African Swine Fever Virus using minimal equipment through a LAMP PCR method

Bohórquez

Lanka²,

Rosell³

et al. 2023

Front. Cell. Infect. Microbiol.

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“…Gordon Spronk, DVM 1 ; Karyn Havas, DVM, PhD, DACVPM 1 ; Gil Patterson, VMD, MPH 2 ; Scott Dee, DVM, PhD, DACVM 1 * different approaches to biosecurity (transboundary vs domestic) are required to achieve success. As the common ground here is freedom from both pathogens, educating the industry on science-based biosecurity principles is needed to properly implement efficacious prevention strategies at the level of both the national herd and local herd.…”

Section: Will Swine Veterinarians Lead By Meeting the Nextgeneration ...mentioning

confidence: 99%

“…A s of this writing, a pathogen known as African swine fever virus (ASFV) is looming on the island of Hispaniola in the Caribbean, 1 721 miles from Miami, Florida. Introduction of ASFV to the US swine national herd would immediately stop the exportation of pork, with long-term projections of $50 billion in losses.…”

mentioning

confidence: 99%

Will swine veterinarians lead by meeting the next-generation needs of our industry?

Spronk

Havas

Patterson³

et al. 2023

javma

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The US swine industry is currently challenged by the potential of transboundary animal disease (eg, African swine fever) entry to the national herd and the relentless pressures of domestic diseases (eg, porcine reproductive and respiratory syndrome). The task of the swine veterinarian is to biosecure both the national herd and their customers’ local farms to mitigate these risks. This Viewpoint raises 4 questions that swine veterinarians, including practicing (private and corporate), industry, research, academic, and regulatory (state and federal) veterinarians who spend a portion of their time controlling, treating, preventing, or eradicating diseases of swine, must answer to meet the needs of their farms to compete globally and survive. In addition, it appears that there is sufficient science-based information to move forward in a collaborative manner and that the goals of prevention of African swine fever and elimination of porcine reproductive and respiratory syndrome virus are technically possible. Therefore, as previous generations of swine veterinarians led the US industry in the elimination of foot-and-mouth disease virus, classical swine fever virus, and pseudorabies virus from the national herd, the central challenge is whether the next generation of veterinarians will provide the necessary leadership to deal with the current industry and its next-generation challenges.

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An integrated fuzzy multi-criteria decision-making model for determining the interdependencies among the african swine fever spread factors

Lauron,

Abellana

2024

Decision Analytics Journal

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Impact of human behavior on the spread of African swine fever virus: what every veterinarian should know

Cited by 4 publications

References 24 publications

Efficient detection of African Swine Fever Virus using minimal equipment through a LAMP PCR method

Efficient detection of African Swine Fever Virus using minimal equipment through a LAMP PCR method

Will swine veterinarians lead by meeting the next-generation needs of our industry?

An integrated fuzzy multi-criteria decision-making model for determining the interdependencies among the african swine fever spread factors

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