2000
DOI: 10.1128/jvi.74.12.5729-5735.2000
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Impact of Human Immunodeficiency Virus Type 1 RNA Dimerization on Viral Infectivity and of Stem-Loop B on RNA Dimerization and Reverse Transcription and Dissociation of Dimerization from Packaging

Abstract: The kissing-loop domain (KLD) encompasses a stem-loop, named kissing-loop or dimerization initiation site (DIS) hairpin (nucleotides [nt] 248 to 270 in the human immunodeficiency virus type 1 strains HIV-1 Lai and HIV-1 Hxb2 ), seated on top of a 12-nt stem-internal loop called stem-loop B (nt 243 to 247 and 271 to 277). Destroying stem-loop B reduced genome dimerization by ϳ50% and proviral DNA synthesis by ϳ85% and left unchanged the dissociation temperature of dimeric genomic RNA. The most affected step of… Show more

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Cited by 77 publications
(92 citation statements)
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“…The precise secondary structure(s) of the SL1 domain have not been definitively established for HIV-2 leader RNA, compared with the homologous region in HIV-1 (see below and Shen et al 2000Shen et al , 2001. Several secondary structure models have been proposed, some of which correspond to the structures predicted in this work.…”
Section: Discussionmentioning
confidence: 99%
“…The precise secondary structure(s) of the SL1 domain have not been definitively established for HIV-2 leader RNA, compared with the homologous region in HIV-1 (see below and Shen et al 2000Shen et al , 2001. Several secondary structure models have been proposed, some of which correspond to the structures predicted in this work.…”
Section: Discussionmentioning
confidence: 99%
“…Notably, the palindromic dimer initiation sites in HIV-1 provide a nucleation point for RNA dimerization, but additional RNA sequences are known to play roles in virion RNA dimerization (Clever et al 1996;Hill et al 2003;Russell et al 2003;Sakuragi et al 2003). HIV-1 mutants with altered dimer initiation sites also have strand-transfer defects during reverse transcription (Paillart et al 1996a;Shen et al 2000). For MoMuLV RNA, two hairpins that form intermolecular interactions and two additional stem-loops that form intermolecular kissing-loop interactions initiate dimerization, while longer self-complementary sequences are believed to stabilize the dimer (Tounekti et al 1992;Oroudjev et al 1999;Kim and Tinoco 2000;Ly and Parslow 2002).…”
Section: Discussionmentioning
confidence: 99%
“…1A). This mutation reduces dimerization in virions by ∼50% (Shen et al 2000). Native gel analysis showed minimal dimer formation by the C258G variant over the range of salt (Supplemental Fig.…”
Section: Role Of Psi Rna Dimerization and Single-stranded G Bases In mentioning
confidence: 99%