Impact of human platelet lysate on the expansion and chondrogenic capacity of cultured human chondrocytes for cartilage cell therapy

Sykes, Jessica Grace; Kuiper, Jan Herman; Richardson, James B.; Wright, Karina; Kuiper, Nicola J.

doi:10.1016/j.joca.2018.02.221

Cited by 5 publications

(5 citation statements)

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“…According to GMP guidelines, animal products should be replaced by human alternatives for clinical translation. 16 Both hPLP and autologous serum turned out to be good options for chondrocyte expansion. However, for research-based experiments hPLP is the more convenient alternative as it can be used as an off-the-shelf product.…”

Section: Discussionmentioning

confidence: 99%

Scaffold-Free Engineering of Human Cartilage Implants

et al. 2021

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Objective Despite new strategies in tissue engineering, cartilage repair remains a major challenge. Our aim is to treat patients with focal lesions of articular cartilage with autologous hyaline cartilage implants using a scaffold-free approach. In this article, we describe experiments to optimize production of scaffold-free cartilage discs. Design Articular chondrocytes were expanded in vitro, seeded in transwell inserts and redifferentiated using established chondrogenic components. Experimental variables included testing 2 different expansion media, adding bone morphogenetic protein 2 (BMP2), insulin-like growth factor 1 (IGF1), growth/differentiation factor 5 (GDF5), or fibroblast growth factor 18 (FGF18) to the differentiation medium and allowing the disc to float freely in large wells. Cartilage discs were analyzed by weight and thickness, real-time RT-qPCR (reverse transcriptase qualitative polymerase chain reaction), fluorescence immunostaining, transmission electron microscopy, second harmonic generation imaging, and measurement of Young’s modulus. Results Addition of BMP2 to the chondrogenic differentiation medium (CDM) was essential for stable disc formation, while IGF1, GDF5, and FGF18 were redundant. Allowing discs to float freely in CDM on a moving platform increased disc thickness compared with discs kept continuously in transwell inserts. Discs cultured for 6 weeks reached a thickness of almost 2 mm and Young’s modulus of >200 kPa. There was abundant type II collagen. Collagen fibrils were 25 nm thick, with a tendency to be organized perpendicular to the disc surface. Conclusion Scaffold-free engineering using BMP2 and providing free movement in CDM produced firm, elastic cartilage discs with abundant type II collagen. This approach may potentially be used in clinical trials.

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Section: Discussionmentioning

confidence: 99%

Scaffold-Free Engineering of Human Cartilage Implants

et al. 2021

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“…However, PL increased follicle survival rate and growth in the antral stage significantly, compared to PLless follicle grafts. PCs including PL, PRP, platelet-rich fibrin, or growth factors alone were confirmed to enhance proliferation, cell survival, and tissue regeneration in cell therapy and tissue engineering [19,39,40]. In a recent study, the ability of PDGF-coated decellularized meniscus scaffold for integrative heal meniscus tears, as the most common knee injury, was examined.…”

Section: Discussionmentioning

confidence: 99%

Fibrin Scaffold Incorporating Platelet Lysate Enhance Follicle Survival and Angiogenesis in Cryopreserved Preantral Follicle Transplantation

Rajabzadeh

Jahanpeyma

Talebi

et al. 2020

GMJ

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Background: Transplantation of cryopreserved follicles can be regarded as a promising strategy for preserving fertility in cancer patients under chemotherapy and radiotherapy by reducing the risk of cancer recurrence. The present study aimed to evaluate whether fibrin hydrogel supplemented with platelet lysate (PL) could be applied to enhance follicular survival, growth, and angiogenesis in cryopreserved preantral follicle grafts. Materials and Methods: Preantral follicles were extracted from 15 four-week-old NMRI mice, cryopreserved by cryotop method, and encapsulated in fibrin-platelet lysate for subsequent heterotopic (subcutaneous) auto-transplantation into the neck. Transplants were assessed in three groups including fresh follicles in fibrin-15%PL, cryopreserved follicles in fibrin-15%PL, and cryopreserved follicles in fibrin-0% PL. Two weeks after transplantation, histological, and immunohistochemistry (CD31) analysis were applied to evaluate follicle morphology, survival rate, and vascular formation, respectively. Results: Based on the results, fibrin-15% PL significantly increased neovascularization and survival rate (SR) both in cryopreserved (SR=66.96%) and fresh follicle (SR=90.8%) grafts, compared to PL-less fibrin cryopreserved transplants (SR=28.46%). The grafts supplemented with PL included a significantly higher percentage of preantral and antral follicles. Also, no significant difference was observed in the percentage of preantral follicles between cryopreserved and fresh grafts of fibrin-15% PL. However, a significantly lower (P=0.03) percentage of follicles (23.37%) increased to the antral stage in cryopreserved grafts of fibrin-15%PL, compared to fresh grafts (35.01%). Conclusion: The findings demonstrated that fibrin-PL matrix could be a promising strategy to improve cryopreserved follicle transplantation and preserve fertility in cancer patients at the risk of ovarian failure. [GMJ.2020;9:e1558]

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“…Similarly, Hildner et al showed that chondrocytes cultured with HPL have better redifferentiation potential compared to those expanded with FBS when comparison was made between cells at the same population doubling number [40]. Contradictory results were reported by Sykes et al whereby the researchers found that chondrocytes cultured with HPL have poorer redifferentiation potential compared to those cultured with FBS [8]. HPL also has been found to reduce the deposition of cartilaginous matrix by chondrocytes in 3D culture [41].…”

Section: Chondrocyte Dedifferentiation and Redifferentiation Of Dedifmentioning

confidence: 95%

“…Importantly, HPL is economical and will not increase the production cost. Similar to FBS, HPL is rich in biological factors that are essential in maintaining chondrocyte survival and proliferation in vitro [8].…”

Section: Introductionmentioning

confidence: 99%

Feasibility of Human Platelet Lysate as an Alternative to Foetal Bovine Serum for In Vitro Expansion of Chondrocytes

Liau

Hassan

Tang

et al. 2021

IJMS

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Osteoarthritis (OA) is a degenerative joint disease that affects a lot of people worldwide. Current treatment for OA mainly focuses on halting or slowing down the disease progress and to improve the patient’s quality of life and functionality. Autologous chondrocyte implantation (ACI) is a new treatment modality with the potential to promote regeneration of worn cartilage. Traditionally, foetal bovine serum (FBS) is used to expand the chondrocytes. However, the use of FBS is not ideal for the expansion of cells mean for clinical applications as it possesses the risk of animal pathogen transmission and animal protein transfer to host. Human platelet lysate (HPL) appears to be a suitable alternative to FBS as it is rich in biological factors that enhance cell proliferation. Thus far, HPL has been found to be superior in promoting chondrocyte proliferation compared to FBS. However, both HPL and FBS cannot prevent chondrocyte dedifferentiation. Discrepant results have been reported for the maintenance of chondrocyte redifferentiation potential by HPL. These differences are likely due to the diversity in the HPL preparation methods. In the future, more studies on HPL need to be performed to develop a standardized technique which is capable of producing HPL that can maintain the chondrocyte redifferentiation potential reproducibly. This review discusses the in vitro expansion of chondrocytes with FBS and HPL, focusing on its capability to promote the proliferation and maintain the chondrogenic characteristics of chondrocytes.

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Impact of human platelet lysate on the expansion and chondrogenic capacity of cultured human chondrocytes for cartilage cell therapy

Cited by 5 publications

References 0 publications

Scaffold-Free Engineering of Human Cartilage Implants

Scaffold-Free Engineering of Human Cartilage Implants

Fibrin Scaffold Incorporating Platelet Lysate Enhance Follicle Survival and Angiogenesis in Cryopreserved Preantral Follicle Transplantation

Feasibility of Human Platelet Lysate as an Alternative to Foetal Bovine Serum for In Vitro Expansion of Chondrocytes

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