Impact of Humidity on In Vitro Human Skin Permeation Experiments for Predicting In Vivo Permeability

Ishida, Masahiro; Takeuchi, Hiroyuki; Endo, Hiromi; Yamaguchi, Junichi

doi:10.1002/jps.24656

Cited by 4 publications

(2 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…secondly, the high hydration of the skin applying 1 mL of physiological solution after the washing procedure can enhance nicotine skin absorption (Ishida et al, 2015), thus overestimate real A C C E P T E D M A N U S C R I P T scenario. Moreover, it should take into account that the toxicological evaluation relies on experimental results claiming/ describing a worstcase scenario.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Skin contamination as pathway for nicotine intoxication in vapers

Maina

Castagnoli

Ghione

et al. 2017

Toxicology in Vitro

View full text Add to dashboard Cite

Growing warnings on health effects related to electronic cigarettes have met inconclusive findings at present. This study analyzed the in vitro percutaneous absorption of nicotine resulting by skin contamination with two e-liquids (refill 1 and 2) containing nicotine at 1.8%. Donor chambers of 6 Franz cells for each refill liquid were filled with 1mL of nicotine e-liquid for 24h; at selected intervals, 1.5mL of the receptor solutions were collected for nicotine concentration analysis by mean gas chromatography-mass spectrometry (LOD: 0.01μg/mL). The experiment was repeated removing the nicotine donor solution after 10min from the application and rinsing the skin surface three times with 3.0mL of milliQ water. A total of 12 cells with 24h exposure and 12 cells washed were studied. The mean concentration of nicotine in the receiving phase at the end of the experiment was 54.9±29.5 and 30.2±18.4μg/cm for refill 1 and 2 respectively and significantly lower in washed cells (4.7±2.4 and 3.5±1.3μg/cm). The skin absorption of nicotine can lead to minor health illness in vapers, while caution must be paid to dermal contamination by e liquids in children. The skin cleaning significantly reduced the transdermal absorption kinetic and intradermal deposition of nicotine.

show abstract

Section: Accepted Manuscriptmentioning

confidence: 99%

Skin contamination as pathway for nicotine intoxication in vapers

Maina

Castagnoli

Ghione

et al. 2017

Toxicology in Vitro

View full text Add to dashboard Cite

show abstract

“…However, the reliability of results from pharmacokinetic and pharmacodynamic (PK/PD) studies performed with dOFM suffers from highly variable skin permeability. The skin permeability to topically applied drugs is mostly determined by the skin's outermost layer, the stratum corneum (SC) (Scheuplein and Blank 1971), and it does not only show high inter and intra-subject variabilities but additionally varies depending on several factors, such as environmental conditions and the kind of the drug applied (Southwell et al 1984, Roskos et al 1989, Akomeah et al 2007, Ishida et al 2015. These skin permeability variations demand large sample and measurement numbers to yield reliable results (Holmgaard et al 2014).…”

Section: Introductionmentioning

confidence: 99%