Impact of injection solvents on supercritical fluid chromatography

Understanding and diminishing the extra-column band broadening effects in supercritical fluid chromatography

“…Several studies highlighted the effect of sample solvents on performance in SFC but even with this knowledge, issues with extra-column band broadening still remain for narrow columns. In general it was found that apolar solvents such as heptane allow to minimize peak distortion, whereas another study found that acetonitrile in combination with water could be used if water-soluble samples are considered [14,15,17,18].…”

A closer study of peak distortions in supercritical fluid chromatography as generated by the injection

“…The solvent strengths for sample solvents according to ε 0 (SiO 2 ) and dielectric constant is presented in Table 1, data from ref. [41].…”

“…Salicylanilide was not chosen due to its apparently more complex adsorption mechanism. To qualitatively Table 1 Properties of solvents used in this study [41]. 1.…”

Theory and Practice of UHPLC and UHPLC–MS

“…Thus, the diluent's dielectric constant, dipole moment, hydrogen bonding, and eluent strength are highly correlated to poor efficiency. Contrarily, injection solvents with higher dielectric constants and dipole moments are suitable for separations on nonpolar stationary phases, such as a C18 column (Abrahamsson and Sandahl, 2013).…”

“…Moreover, acetonitrile is not retained and does not interfere with analytes. Other commonly used solvents, dimethylsulfoxide and dimethylformamide, are inconvenient in SFC because of distorted peaks observed even at low injection volumes, important absorption in the UV region, and retention on polar columns resulting in interfering peaks (Abrahamsson and Sandahl, 2013;Fairchild et al, 2013). been tested for interfacing SFC and MS, including direct fluid introduction, the moving belt interface, the thermospray interface, the particle beam interface, plasma ion sources, such as microwave-induced plasma and inductively coupled plasma, and finally atmospheric pressure ionization sources, namely ESI and atmospheric pressure chemical ionization (APCI).…”