Impact of inorganic nitrogen additions on microbes in biological soil crusts

Wang, Jin; Bao, Jing-Ting; Su, Jieqiong; Li, Xinrong; Chen, Guoxiong; Ma, Xiaofei

doi:10.1016/j.soilbio.2015.06.004

Cited by 90 publications

(54 citation statements)

References 81 publications

(103 reference statements)

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“…Actinobacteria and Proteobacteria are usually predicted to be copiotrophic groups which increase in high-C environments (Fierer et al, 2007). These results differ from those reported in BSCs from Oman and the Gurbantunggut Desert (Abed et al, 2010;Moquin et al, 2012;Zhang et al, 2016), and even from BSCs of natural vegetation at the edge of the Tengger Desert (Wang et al, 2015), where Proteobacteria were the most abundant phylum, followed by Cyanobacteria, Actinobacteria and Chloroflexi. Unexpectedly, Cyanobacteria had a high proportion in the developed BSCs, although they were prevalent in early successional stages of BSCs (5YR) and play crucial roles in initial crust development (Belnap and Lange, 2001).…”

Section: Impact Of Bsc Age On Bacterial Community Compositioncontrasting

confidence: 57%

“…This is relatively similar to that in the natural habitat around the Tengger Desert, where Cyanobacteria (19.5 %) and Actinobacteria (19.4 %) were the most dominant phyla after Proteobacteria (25.0 %). Moreover, the results did not resemble those from arid Arizona soils (Dunbar et al, 1999) or the Gurbantunggut Desert (Zhang et al, 2016) due to the high proportion of Chloroflexi, an unexplained presence of thermophilic phyla (Gundlapally and Garcia-Pichel, 2006;Moquin et al, 2012;Nagy et al, 2005) that display good adaptation to drought conditions and the important roles in the development of BSCs in arid zones (Lacap et al, 2011;Wang et al, 2015). MS,5YR,15YR,28YR,34YR and 51YR represent mobile sand,respectively. …”

Section: Impact Of Bsc Age On Bacterial Community Compositionmentioning

confidence: 89%

“…PCR analyses were performed in triplicate 20 µL mixture containing 2 µL of 5 × FastPfu Buffer, 2 µL of 2.5 mM dNTPs, 0.8 µL of each primer (5 µM), 0.2 µL of FastPfu Polymerase and 10 ng of template DNA. This was conducted according to Wang et al (2015). Amplicons were extracted from 2 % agarose gels and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer's instructions and quantified using QuantiFluor ™ -ST (Promega Corporation, Madison, WI, USA).…”

Section: Dna Extraction and Illumina Miseq Sequencingmentioning

confidence: 99%

“…Bacteria present the highest proportion of the microbial biomass in BSCs (Bates et al, 2010;Green et al, 2008;Gundlapally and Garcia-Pichel, 2006;Maier et al, 2014;Wang et al, 2015) and thus have important roles in the BSC successional process. They can decompose organic material and release nutrients, mediating geochemical processes necessary for ecosystem functioning in the persistence of BSCs (Balser and Firestone, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Development of bacterial communities in biological soil crusts along a revegetation chronosequence in the Tengger Desert, northwest China

Liu

Zhang

et al. 2017

Biogeosciences

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Abstract. Knowledge of structure and function of microbial communities in different successional stages of biological soil crusts (BSCs) is still scarce for desert areas. In this study, Illumina MiSeq sequencing was used to assess the compositional changes of bacterial communities in different ages of BSCs in the revegetation of Shapotou in the Tengger Desert. The most dominant phyla of bacterial communities shifted with the changed types of BSCs in the successional stages, from Firmicutes in mobile sand and physical crusts to Actinobacteria and Proteobacteria in BSCs, and the most dominant genera shifted from Bacillus, Enterococcus and Lactococcus to RB41_norank and JG34-KF-361_norank. Alpha diversity and quantitative real-time polymerase chain reaction (PCR) analysis indicated that bacterial richness and abundance reached their highest levels after 15 years of BSC development. Redundancy analysis showed that silt + clay content and total K were the prime determinants of the bacterial communities of BSCs. The results suggested that bacterial communities of BSCs recovered quickly with the improved soil physicochemical properties in the early stages of BSC succession. Changes in the bacterial community structure may be an important indicator in the biogeochemical cycling and nutrient storage in early successional stages of BSCs in desert ecosystems.

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Section: Impact Of Bsc Age On Bacterial Community Compositioncontrasting

confidence: 57%

Section: Impact Of Bsc Age On Bacterial Community Compositionmentioning

confidence: 89%

Section: Dna Extraction and Illumina Miseq Sequencingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of bacterial communities in biological soil crusts along a revegetation chronosequence in the Tengger Desert, northwest China

Liu

Zhang

et al. 2017

Biogeosciences

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“…The control (mobile sand), the artificially revegetating sites restored since 1981 and 1956, respectively, and the naturally revegetating site are referred to as NCS, N81S, N56S, and NNS for DNA-level analysis and as NCSR, N81SR, N56SR, and NNSR for transcript-level analysis, respectively facilitation to dominant competition once the BSCs formed. Alternatively, although diazotrophs usually give way to other species over time due to increases in soil N availability [3], the increases in soil N availability in the revegetated sites (Table 1) may more favor cyanobacteria relative to others as suggested by Wang et al [68], resulting in the predominance of cyanobacterial diazotrophs in the revegetated sites.…”

Section: Discussionmentioning

confidence: 91%

Molecular Ecology of nifH Genes and Transcripts Along a Chronosequence in Revegetated Areas of the Tengger Desert

Wang

Bao

et al. 2015

Microb Ecol

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The colonization and succession of diazotrophs are essential for the development of organic soil layers in desert. We examined the succession of diazotrophs in the well-established revegetated areas representing a chronosequence of 0 year (control), 22 years (restored artificially since 1981), 57 years (restored artificially since 1956), and more than 100 years (restored naturally) to determine the community assembly and active expression of diazotrophs. The pyrosequencing data revealed that Alphaproteobacteria-like diazotrophs predominated in the topsoil of our mobile dune site, while cyanobacterial diazotrophs predominated in the revegetated sites. The cyanobacterial diazotrophs were primarily composed of the heterocystous genera Anabaena, Calothrix, Cylindrospermum, Nodularia, Nostoc, Trichormus, and Mastigocladus. Almost all the nifH sequences belonged to the Cyanobacteria phylum (all the relative abundance values >99.1 %) at transcript level and all the active cyanobacterial diazotrophs distributed in the families Nostocaceae and Rivulariaceae. The most dominant active cyanobacterial genus was Cylindrospermum in all the samples. The rank abundance and community analyses demonstrated that most of the diazotrophic diversity originated from the "rare" species, and all the DNA-based diazotrophic libraries were richer and more diverse than their RNA-based counterparts in the revegetated sites. Significant differences in the diazotrophic community and their active population composition were observed among the four research sites. Samples from the 1981-revegetating site (predominated by cyanobacterial crusts) showed the highest nitrogenase activity, followed by samples from the naturally revegetating site (predominated by lichen crusts), the 1956-revegetating site (predominated by moss crusts), and the mobile dune site (without crusts). Collectively, our data highlight the importance of nitrogen fixation by the primary successional desert topsoil and suggest that the N2-fixing cyanobacteria are the key diazotrophs to the nitrogen budget and the development of topsoil in desert, which is critical for the succession of the degraded terrestrial ecosystems.

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High nitrogen rates do not increase canola yield and may affect soil bacterial functioning

et al. 2020

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Nitrogen fertilization has a fundamental role in agricultural productivity. However, injudicious N applications to crops are common. It is important to ensure the minimum N required for satisfactory crop growth is applied but that excess amounts are avoided due to potential impacts on agroecosystem functioning. Nitrogen at 0, 60, and 150 kg ha–1 was applied as limestone ammonium nitrate to plots arranged in a randomized complete block design, on three farms to determine the impact of rate and temporal distribution of fertilizer on canola (Brassica napus L.) production in South Africa, and the effect of N fertilizer application on the composition and diversity of soil bacterial communities. The amount and distribution of N had only minor effects on canola growth (P < .05) and no effects on yield or harvest index. Splitting fertilizer into two or three applications throughout the season resulted in more mineral N available in the soil later in the season. Increasing the N rate from 60 to 150 kg ha–1 had a significant impact on bacterial community composition. The lower rate favored bacteria that are more able to break down N‐containing C sources. No effects of fertilizer amount or distribution were observed on either N fixation potential (number of nifH gene copies) or bacterial community diversity. Overall, a low rate of N fertilizer split into multiple applications is recommended for canola production, as higher rates do not increase yield and may have a detrimental impact on soil C and N cycling.

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Impact of inorganic nitrogen additions on microbes in biological soil crusts

Cited by 90 publications

References 81 publications

Development of bacterial communities in biological soil crusts along a revegetation chronosequence in the Tengger Desert, northwest China

Development of bacterial communities in biological soil crusts along a revegetation chronosequence in the Tengger Desert, northwest China

Molecular Ecology of nifH Genes and Transcripts Along a Chronosequence in Revegetated Areas of the Tengger Desert

High nitrogen rates do not increase canola yield and may affect soil bacterial functioning

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