Impact of intercellular crosstalk between epidermal keratinocytes and dermal fibroblasts on skin homeostasis

Jevtić, Marijana; Löwa, Anna; Nováčková, Anna; Kováčik, Andrej; Kaessmeyer, Sabine; Erdmann, Gerrit; Vávrová, Kateřina; Hedtrich, Sarah

doi:10.1016/j.bbamcr.2020.118722

Cited by 44 publications

(38 citation statements)

References 37 publications

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“…When scars, burns, and wounds are not able to heal on their own, they require replacement of the dermal barrier. It is very common to find skin equivalent and reconstruction research in the literature using primary human fibroblasts and keratinocytes, regularly supplemented with a collagen type I matrix or ascorbate [127,128]. In terms of decellularized approaches, acellular dermal matrices (ADMs) are widely used in clinical regenerative medicine approaches because of their biological and structural organization.…”

Section: Dermal Tissuementioning

confidence: 99%

Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds

Mendibil

Ruiz-Hernández

Retegi-Carrión

et al. 2020

IJMS

203

187

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The extracellular matrix (ECM) is a complex network with multiple functions, including specific functions during tissue regeneration. Precisely, the properties of the ECM have been thoroughly used in tissue engineering and regenerative medicine research, aiming to restore the function of damaged or dysfunctional tissues. Tissue decellularization is gaining momentum as a technique to obtain potentially implantable decellularized extracellular matrix (dECM) with well-preserved key components. Interestingly, the tissue-specific dECM is becoming a feasible option to carry out regenerative medicine research, with multiple advantages compared to other approaches. This review provides an overview of the most common methods used to obtain the dECM and summarizes the strategies adopted to decellularize specific tissues, aiming to provide a helpful guide for future research development.

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Section: Dermal Tissuementioning

confidence: 99%

Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds

Mendibil

Ruiz-Hernández

Retegi-Carrión

et al. 2020

IJMS

203

187

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“…The skin is a complex organ, with a structure consisting of two main tissue layers: the overlying epidermis, which primarily contains keratinocytes, and the underlying dermis, a connective tissue that comprises collagens and fibers, along with multiple cell types such as fibroblasts, endothelial cells, macrophages, and mast cells ( 4 , 5 ). Previous studies demonstrated that these skin cells are responsible for maintaining healthy skin tissue homeostasis and are critically involved in wound healing.…”

Section: Introductionmentioning

confidence: 99%

High Glucose Causes Distinct Expression Patterns of Primary Human Skin Cells by RNA Sequencing

Zhang

Yang

et al. 2021

Front. Endocrinol.

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Diabetes-related skin problems represent the most common long-term complications in diabetes mellitus patients. These complications, which include diabetic dermopathy, diabetic blisters, necrobiosis lipoidica diabeticorum, and eruptive xanthomatosis, may dramatically impair patients’ quality of life and cause long-lasting disability. However, the cellular and molecular mechanisms linking diabetes-related hyperglycemia and skin complications are still incompletely understood. To assess the role of the various skin-cell types in hyperglycemia-induced skin disorders, we performed RNA sequencing-based transcriptome analysis, measuring gene expression patterns in biological replicates in normal- and high glucose-stimulated skin cells. Three primary human skin-cell types were examined, i.e., epidermal keratinocytes, dermal fibroblasts, and dermal microvascular endothelial cells. For each separate cell type, we identified gene expression. Comparing gene abundances and expression levels revealed that transcription profiles exhibit distinct patterns in the three skin-cell types exposed to normal (i.e., physiological) glucose treatment and high (i.e., supraphysiological) glucose treatment. The obtained data indicate that high glucose induced differential gene expression and distinct activity patterns in signaling pathways in each skin-cell type. We are adding these data to the public database in the hope that they will facilitate future studies to develop novel targeted interventions for diabetic skin complications.

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“…Reports confirm that mesenchymal-epithelial cross talk when mediated by autocrine/paracrine regulatory mechanisms initiate keratinocyte-fibroblast interaction, development and differentiation which controls the MMP expression and therefore, help achieve skin homeostasis [41,46]. Keratinocytes, in a typical skin, are accumulated within the dormant epidermal tissue adjoined by the desmosomal-hemidesmosomal cell junctions.…”

Section: Crosstalk Of Keratinocytes and Fibroblasts During Healingmentioning

confidence: 97%

“…The proliferative phase consists of the granulation tissue development from the interim wound bed formed during the haemostatic phase. The granulation tissue comprises of a pool of fibroblasts, white blood cells, phagocytic cells, blood vessel networks and collagen bundles that recuperates the structural and functional integrity of the damaged tissue [41]. Fibroblasts exhibit significant role in preserving skin's homeostatic balance and for orchestrating granulation tissue formation.…”

Section: Inflammatory Cellular Response and Its Transition From Inflammation To Reepithelization And Refurbishmentmentioning

confidence: 99%

“…Fibroblasts exhibit significant role in preserving skin's homeostatic balance and for orchestrating granulation tissue formation. Upon its migration into the interim dermis wound bed, these activated fibroblasts proliferate and secrete MMP's for simultaneous degradation of wound matrix [41] and remodelling of ECM ensuring wound closure. Myofibroblasts are capable of cell adhesion, growth, division, apoptosis with growth factor bioavailability by binding, sequestration, and initiation during the repair process [42].…”

Section: Inflammatory Cellular Response and Its Transition From Inflammation To Reepithelization And Refurbishmentmentioning

confidence: 99%

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Nanoceutical Adjuncts as Wound Healing Material: Precepts and Prospects

Madhyastha¹,

Banerjee²,

Madhyastha³

et al. 2021

Preprint

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Dermal wound healing describes the progressive repair and recalcitrant mechanism of damaged skin and eventually reformatting and reshaping the skin. Many probiotics, nutraceuticals, metal nanoparticles have been associated with improved healing process of intra and inter tissue wounds. Despite the vast nature on material based wound healing mediators, the exact mechanism on material-cellular interaction is still point of repent issue particularly in diabetics and pathological condition. The use of bioengineered alternative agents will likely only continue to dominate the outpatient and perioperative management of chronic, recalcitrant wounds as new additional products continue to cut costs and improve wound healing process. This review article provides an update of the various remedies with confirmed wound healing activities by a diverse group of agents from previous experiments conducted by various researchers

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Impact of intercellular crosstalk between epidermal keratinocytes and dermal fibroblasts on skin homeostasis

Cited by 44 publications

References 37 publications

Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds

Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds

High Glucose Causes Distinct Expression Patterns of Primary Human Skin Cells by RNA Sequencing

Nanoceutical Adjuncts as Wound Healing Material: Precepts and Prospects

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