Impact of macrophages on tumor growth characteristics in a murine ocular tumor model

Stei, Marta M.; Loeffler, Karin U.; Kurts, Christian; Hoeller, Tobias; Pfarrer, Christiane; Holz, Frank G.; Herwig‐Carl, Martina C.

doi:10.1016/j.exer.2016.07.008

Cited by 5 publications

(7 citation statements)

References 40 publications

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“…The ability to examine the interaction between tumor and host cells as the cancer progresses in an immunocompetent animal is arguably the greatest strength of this model. Additionally, recipient mice may be genetically altered in order to study specific contributions of the host in melanoma progression (Lattier, Yang, Crawford, & Grossniklaus, 2013;Stei, Loeffler, Kurts, et al, 2016).…”

Section: Syng Eneic Cutaneous Mel Anoma Mous E Model S For S Imul Ati...mentioning

confidence: 99%

Mouse models of uveal melanoma: Strengths, weaknesses, and future directions

Richards

Yoo

Shin

et al. 2020

Pigment Cell Melanoma Res

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Uveal melanoma is the most common primary malignancy of the eye, and a number of discoveries in the last decade have led to a more thorough molecular characterization of this cancer. However, the prognosis remains dismal for patients with metastases, and there is an urgent need to identify treatments that are effective for this stage of disease. Animal models are important tools for preclinical studies of uveal melanoma. A variety of models exist, and they have specific advantages, disadvantages, and applications. In this review article, these differences are explored in detail, and ideas for new models that might overcome current challenges are proposed.

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Section: Syng Eneic Cutaneous Mel Anoma Mous E Model S For S Imul Ati...mentioning

confidence: 99%

Mouse models of uveal melanoma: Strengths, weaknesses, and future directions

Richards

Yoo

Shin

et al. 2020

Pigment Cell Melanoma Res

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“…M.M. Stei et al описали факторы неблагоприятного прогноза УМ, к которым отнесли пожилой возраст пациентов, а также появление туморинфильтрирующих макрофагов (TИM) и вы-сокую плотность кровеносных сосудов в опухоли [17]. При этом показано, что на прогрессирование опухоли могут влиять макрофаги популяции М2 и уровень экспрессии их цитокинов.…”

Section: Introductionunclassified

“…При этом показано, что на прогрессирование опухоли могут влиять макрофаги популяции М2 и уровень экспрессии их цитокинов. Авторами установлено, что прогрессирование опухоли достоверно коррелирует с количеством макрофагов М2 in vivo и in vitro [17].…”

Section: Introductionunclassified

Prognostic value of macrophage vasculiform mimicry in the evaluation of uveal melanoma progression

Shamanova

Kazachkov

Панова

et al. 2023

jour

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Introduction Uveal melanoma (UM) is a malignant neoplasm of the vascular tract of the eye, prone to hematogenous metastasis, regardless of the type of treatment performed. Prediction and probability of UM metastasis is one of the main tasks of onco-ophthalmology. We decribed an alternative method of tumor blood supply due to the process of vasculo-like mimicry (VM) with the formation of pseudovascular structures of tumor cells is described. At the same time, the participation of the microenvironment in the processes of VM and metastasis is currently debatable and relevant.Purpose of the work was to evaluate the prognostic role of macrophage vasculiform mimicry in the progression of chorioid melanoma based on the histological and immunohistochemical characteristics of surgical material.Materials and methods The surgical material of choroidal melanoma after enucleation for the period 2013-2018 was studied. Histological, histochemical (PAS reaction) and immunohistochemical studies were carried out using markers CD68 (macrophages), CD 34, CD31, D2-40 for the purpose of qualitative and quantitative assessment of macrophages in the stroma of tumors, large vessels and pseudovessels in the tumor.Results The ability of macrophages to form vasculo-like spaces in choroidal melanoma has been discovered and described. In growth with macrometastases, more phage representatives are recorded than in tissue samples of non-metastasizing melanoma. the volume of the macroscopic mass of the ulcer and the growth rate of phages were registered as the inverse average in terms of the strength of the correlation. In group studies with metastases, an increase in the volume of pseudovascular volumes and the number of phages is recorded.Discussion The macrophage microenvironment in a tumor can play a dual role: antitumor and protumor. The ability of macrophages for VM in UM has been described. The results of our macrostudy confirm the protumor activity of phages and their important role in tumor metastasis.Conclusion In-depth study of the ways of pseudovascular space formation not only by the cells of the tumor itself, but also by its microenvironment, helps to open new directions in the study of predictors of UM metastasis and outline approaches to the development of new directions of anti-tumortherapy.

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“…В публикации A. Javed et al (2020) [26] показано, что лимфоцитарное микроокружение клеток УМ стоит рассматривать в качестве потенциальных «защитников» от опухоли, и клетки микроокружения, в том числе натуральные киллеры, NK-клетки, особенно во вторичных очагах опухоли (метастазы в печени) могут рассматриваться как перспективные клетки для провоопухолевого воздействия [26]. Активно исследуется роль клеточного микроокружения при ЗНО различных локализаций, в том числе макрофагов [27][28]. По данным M.M.…”

Section: Introductionunclassified

“…По данным M.M. Stei (2016) [27], показано, что туморинфильтрирующие макрофаги (TИM), средняя плотность сосудов в УМ, а также PAS-положительные паттерны экстраваскулярного матрикса этой опухоли и пожилой возраст пациентов связаны с плохим прогнозом при увеальной меланоме [27].…”

Section: Introductionunclassified

Features of the lymphocytic microenvironment in metastatic uveal melanoma

Шаманова

Kazachkov

Панова

et al. 2021

jour

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Introduction. Uveal melanoma is a malignant neoplasm of the vascular tract of the eye. Prevention of metastasis of this tumor is one of the main tasks in order to increase the rates of relapse-free survival of patients. Despite the pronounced immunosuppressive activity of uveal melanoma cells, its lymphocytic microenvironment exerts its antitumor effect.Aim of the study. Compare the lymphocytic microenvironment of primary uveal melanomas and distant metastases (to the liver).Мaterials and methods. The tissue material of choroid melanoma after enucleation and the material of tumor metastases for the period 2013-2018 were studied. An immunohistochemical study was performed using CD8, CD4, and CD56 markers for the qualitative and quantitative assessment of lymphocytes in the tumor stroma.Results. Differences were found in the lymphocytic infiltration of the uveal melanoma stroma and its distant metastases. A statistically significantly greater representation of CD4, CD8-lymphocytes and CD56 cells in tumor metastases than in primary melanoma tissue samples, with CD4-lymphocytes predominant. A direct high-strength correlation was registered between the number of CD4-lymphocytes and CD8-lymphocytes.Discussion. Malignant cells actively modify their cellular and stromal-vascular environment, ensuring their active growth and reproduction. The question of the immune reactivity of the surrounding cells in relation to uveal melanoma remains debatable. According to our data, which is consistent with a number of other studies, uveal melanoma cells do not completely evade the body's immune response. Thus, the determination of possible points of antitumor exposure can be based on a detailed study of the microenvironment of uveal melanoma. Conclusions. The pronounced lymphocytic infiltrate found in uveal melanoma metastases in comparison with the primary tumor indicates an active immune response of the body to the tumor. These results of our study confirm the importance of further studying the immune-mediated antitumor effect on uveal melanoma and the need to investigate possible approaches to immunotherapy.

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Impact of macrophages on tumor growth characteristics in a murine ocular tumor model

Cited by 5 publications

References 40 publications

Mouse models of uveal melanoma: Strengths, weaknesses, and future directions

Mouse models of uveal melanoma: Strengths, weaknesses, and future directions

Prognostic value of macrophage vasculiform mimicry in the evaluation of uveal melanoma progression

Features of the lymphocytic microenvironment in metastatic uveal melanoma

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