Impact of medicated feed along with clay mineral supplementation on Escherichia coli resistance to antimicrobial agents in pigs after weaning in field conditions

Jahanbakhsh, Seyedehameneh; Kabore, Kiswendsida Paul; Fravalo, Philippe; Letellier, Ann; Fairbrother, John M.

doi:10.1016/j.rvsc.2015.07.014

Cited by 29 publications

(29 citation statements)

References 35 publications

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“…A similar co-selection of sulphonamide resistance genes was reported in chickens treated with streptomycin [32]. Moreso, a Canadian study found a predominant pattern of AMR with extended-spectrum cephalosporin (ESC)-resistant E. coli strains, with co-resistance to streptomycin, cefoxitin, trimethoprim-sulfamethoxazole, sulfisoxazole, ampicillin, amoxicillin/clavulanic acid, chloramphenicol, and tetracycline [30]. Also, in an extensive study of E. coli isolates from various countries authors observed that nearly 75% of ampicillin-resistant E. coli isolates were also resistant to streptomycin and tetracycline [33].…”

Section: Discussionmentioning

confidence: 62%

Prevalence and distribution of antimicrobial resistance determinants ofEscherichia coliisolates obtained from meat in South Africa

Jaja

Oguttu

Green

et al. 2019

Preprint

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28Objective: This study aimed to characterise antibiotics resistance of Escherichia coli isolates 29 from the formal meat sector (FMS) and informal meat sectors (INMS). 30Method: A total of 162 and 102 E. coli isolates from the FMS, and INMS respectively were 31 isolated by standard culture-based, and biochemical reactions. The isolates were further 32 confirmed by polymerase chain reaction (PCR). The disc diffusion method was used to 33 screen for antimicrobial susceptibility against 19 different antibiotics. The presence of class 34 1-2 integrons in each E. coli isolates was assessed using 3-CS and 5-CS regions specific 35 primers. 36Result: Among the 19 antimicrobials, resistance to tetracyclines, aminoglycosides, 37 cephalosporins, and nitrofurans were found to be more frequent than carbapenems and 38 phenicol with a noticeable increase in the number of multi-drug resistance ranging from three 39 to ten antimicrobials. A total of 20 resistance determinants were assessed with their 40 prevalence and distributions obtained as follows for FMS and INMS respectively; 41 [aminoglycosides: aadA (40.6%; 31.9%), aacC2 (21.4%; 31%), aphA1 (20.8%; 15.1%), 42 aphA2 (37.7%; 18.9%) and strA (6.5%; 9.4%)], [β-lactams: ampC (20%; 45%), blaTEM, 43 (4.4%; 13.3), and blaZ (8.9%; 2.2%)], [Chloramphenicol: catI (1.7%; 1.7%), and cmIA1 44 (1.7%; 1.7%)] and [tetracyclines: tetA (7.7%; 15.4%), tetB (11.5%; 24%), and tetM, (1.9%; 45 8.7%)], and [sulfonamides: sul1 (22.2%; 26.7%), sul2 (17.8%; 6.7%)]. 46 Conclusion: Multiple antibiotic resistance (MAR) indexes ranged from 0.2 -0.5. The results 47 reveal a high prevalence of multidrug-resistant E. coli isolates and resistance determinants 48 suggesting that consumers and handlers of such meat are at risk of contracting antibiotic 49 resistant E. coli-related foodborne disease. 50 51 1 Background 53Meat is a high-risk food, and many studies have shown a causal relationship between meat 54 consumption and disease outbreaks. Meat-related foodborne diseases frequently occur due to 55 the consumption of Escherichia coli contaminated raw or poorly processed meat. The United 56 States, Center for Disease Control and Prevention (CDC) estimates for the year 2011 that one 57 in six or forty-eight million people are infected with a foodborne illness each year, resulting 58 in 3000 deaths. Of this number, E. coli O157: H7 caused an estimated 73480 illnesses each 59 year, resulting in more than 2000 hospitalisations and 60 deaths [1]. 60 Even though raw food hygiene and food safety epidemiology is still in its infancy in Africa, 61 foodborne disease (FBD) is common in developing countries. For instance, 10200 cases of 62 Shiga toxin E.coli (STEC) related FBD was reported in WHO subregion AFR D and E circa 3 63 2012 [2]. The actual prevalence of food-borne infections is difficult to determine, primarily 64 because only a small percentage of incidence is officially reported. Even when cases of 65 foodborne infections are reported, only in a limited number is the aetiology determined [3]. 6...

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Section: Discussionmentioning

confidence: 62%

Prevalence and distribution of antimicrobial resistance determinants ofEscherichia coliisolates obtained from meat in South Africa

Jaja

Oguttu

Green

et al. 2019

Preprint

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“…Isolates were tested for susceptibility to the 14 antimicrobial agents examined in the Canadian Integrated Program for Antimicrobial Surveillance (CIPARS 2008) using the disk‐diffusion assay as previously described . The following antimicrobial disks were used: azithromycin (15 μg), streptomycin (STR; 10 μg), gentamicin (GEN; 10 μg), trimethoprim‐sulfamethoxazole (23.75 μg), sulfisoxazole (SSS; 250 μg), tetracycline (TET; 30 μg), nalidixic acid (NAL; 30 μg), ciprofloxacin (CIP; 5 μg), chloramphenicol (CHL; 30 μg), ampicillin (AMP; 10 μg), amoxicillin/clavulanic acid (AMC; 30 μg), cefoxitin (FOX; 30 μg), ceftriaxone (CRO; 30 μg), and XNL (30 μg).…”

Section: Methodsmentioning

confidence: 99%

Prevalence, risk factors, and characterization of multidrug resistant and extended spectrum β‐lactamase/AmpC β‐lactamase producing Escherichia coli in healthy horses in France in 2015

Lagarde

Larrieu

Praud

et al. 2019

Veterinary Internal Medicne

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Background Although antimicrobial resistance is increasingly common in equine medicine, molecular and epidemiological data remains scarce. Objectives We estimated the prevalence of, and risk factors for, shedding of multidrug resistant (MDR), extended spectrum β‐lactamase (ESBL)‐producing, and AmpC β‐lactamase‐producing, or some combination of these in Escherichia coli in horses in France. We characterized ESBL/AmpC isolates for antimicrobial susceptibility and the presence of virulence and ESBL/AmpC‐associated resistance genes. Animals Fecal samples from healthy adult horses at 41 premises were collected. A questionnaire was completed by each premises manager. A subset of these samples was tested to build 2 bacterial collections. Methods Indicator (without enrichment) and specific (enrichment with ceftriaxone) E. coli tested for antimicrobial susceptibility. Prevalence of isolates nonsusceptible to antimicrobials was estimated at the horse and the premises level. The ESBL/AmpC and virulence genes were identified by PCR. Multivariable logistic regression was used to investigate risk factors for MDR and ESBL/AmpC isolates at premises. Results Approximately 44% of horses shed MDR E. coli. Resistance most commonly was observed to ampicillin, streptomycin, and amoxicillin/clavulanic acid. Twenty‐nine percent of premises housed horses shedding ESBL/AmpC‐producing isolates. The ESBL/AmpC gene most commonly identified was blaCTX‐M‐1. Virulence gene iutA was identified in 1 ESBL/AmpC‐producing isolate. Medical treatment, staff numbers, and activity were identified as risk factors for housing horses shedding ESBL/AmpC‐producing E. coli isolates. Conclusions and Clinical Importance Prevalence of healthy horses harboring ESBL/AmpC genes and MDR isolates in their intestinal microbiota is substantial. Risk factors could be used to elaborate guidelines to prevent their dissemination.

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“…coli isolates were examined for susceptibility to the same 15 antimicrobial agents examined in the Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS) surveillance programme in Canada [11] using the disk diffusion (Kirby-Bauer) assay as previously described [12]. E. coli isolates were considered MDR based on the definitions described by Magiorakos et al (nonsusceptibility to at least one antimicrobial agent in three or more antimicrobial-defined categories) [13].…”

Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

Dynamics of extended-spectrum cephalosporin resistance in pathogenic Escherichia coli isolated from diseased pigs in Quebec, Canada

Jahanbakhsh

Smith

Kohan-Ghadr

et al. 2016

International Journal of Antimicrobial Agents

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Impact of medicated feed along with clay mineral supplementation on Escherichia coli resistance to antimicrobial agents in pigs after weaning in field conditions

Cited by 29 publications

References 35 publications

Prevalence and distribution of antimicrobial resistance determinants ofEscherichia coliisolates obtained from meat in South Africa

Prevalence and distribution of antimicrobial resistance determinants ofEscherichia coliisolates obtained from meat in South Africa

Prevalence, risk factors, and characterization of multidrug resistant and extended spectrum β‐lactamase/AmpC β‐lactamase producing Escherichia coli in healthy horses in France in 2015

Dynamics of extended-spectrum cephalosporin resistance in pathogenic Escherichia coli isolated from diseased pigs in Quebec, Canada

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