Impact of Microbial Protease Enzyme and Dietary Crude Protein Levels on Growth and Nutrients Digestibility in Broilers over 15–28 Days

Jabbar, Abdul; Tahir, Muhammad Naeem; Alhidary, Ibrahim A.; Abdelrahman, Mutassim A.; Albadani, Hani; Khan, Rifat Ullah; Selvaggi, Maria; Laudadio, Vito; Tufarelli, Vincenzo

doi:10.3390/ani11092499

Cited by 29 publications

(20 citation statements)

References 20 publications

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“…The banning of antibiotics as growth promoters, which has triggered research into alternative to antibiotics, such as probiotics [ 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ], prebiotics [ 11 , 12 , 13 , 14 ], enzymes [ 15 , 16 , 17 , 18 ], organic acids [ 19 , 20 ], herbs [ 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ] and other compounds [ 37 , 38 ], has been reported in poultry production ( Figure 1 ). Researchers are developing phytogenics for usage in chickens with many advantages, such as increased feed efficiency, improved immunological status, and improved gastrointestinal health, in addition to others [ 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 ].…”

Section: Introductionmentioning

confidence: 99%

Potential Applications of Moringa oleifera in Poultry Health and Production as Alternative to Antibiotics: A Review

Khan

Khan²,

Naz

et al. 2021

Antibiotics

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Because of developing bacterial resistance and increased public awareness of health and food safety problems, the use of antibiotics as growth promoters in the chicken industry has been outlawed. This problem has spurred the poultry industry and sector to explore for safe antibiotic alternatives and to focus on developing better long-term feed management solutions in order to improve chicken health and growth. As a result, phytogenics have developed as natural antibiotic alternatives, with a lot of potential in the poultry industry. Moringa oleifera has gotten a lot of attention from researchers in the recent past as a natural product with a lot of health advantages for poultry. Moringa is known for its antimicrobial, antioxidant, anti-inflammatory, and hypocholesterolemic properties, as well as its capacity to activate digestive enzymes in the stomach, owing to the presence of hundreds of essential ingredients. The potential influence of M. oleifera as a natural feed supplement on overall gut health, nutritional digestibility, blood biochemical profile, antioxidant benefits, antibacterial potential, and immunological response is emphasized in this review.

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Section: Introductionmentioning

confidence: 99%

Potential Applications of Moringa oleifera in Poultry Health and Production as Alternative to Antibiotics: A Review

Khan

Khan²,

Naz

et al. 2021

Antibiotics

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“…2 ProAct360 ® , DSM Nutritional Products, Switzerland; 50 g/ton corn diluted premixes had 0, 17 or 50 of the protease corresponding to 0, 10,000 and 30,000 New Feed Protease (NFP) units. Vieira et al 10.3389/fanim.2022.1040051 effects (Kaczmarek et al, 2014;Yuan et al, 2017;Walk et al, 2019) to significant improvements in growth performance of broilers (Freitas et al, 2011;Lee et al, 2018;Jabbar et al, 2021;McCafferty et al, 2022). Synergy between proteases with other in feed supplemental enzymes, such as phytases, xylanases and amylases have also been reported (Cowieson and Adeola, 2005;Amerah et al, 2017;Cowieson et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Growth performance and nutrient digestibility of broiler chickens as affected by a novel protease

et al. 2023

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Two experiments evaluated the addition of an exogenous sfericase protease in broiler diets. Experiments were run (Exp1 and Exp2) with 1,848 and 2,100 one-day-old male chicks being allocated into 84 floor pens with 14 replicates of 22 and 25 birds each, respectively. The studies were conducted in completely randomized designs. In Exp1, Standard diets were formulated with energy and AA at marginally lower levels than usual by the Brazilian integration such that broilers were expected to grow at comparatively reduced rates to the industry whereas in Exp2, the Standard diets were formulated using energy and AA as usual by the Brazilian integrations such that broilers were expected to grow comparable to industry rates. Standard diets had ideally balanced amino acids (AA). Matrix diets, in contrast, had reductions of 6% digestible lysine and of 20 kcal AME/kg compared to the Standard. Matrix diets were supplemented with an sfericase protease at 0, 10,000, and 30,000 New Feed Protease units (NFP)/kg. Outcomes showed no interaction between diet and protease in any of the experiments. However, broilers fed Standard diets had higher cumulative body weight gain (BWG) to 35 and 42 d when compared to Matrix fed birds whereas FCR were worse for birds fed the Matrix diets at 35 d in EXP1 and at 35 and 42 d in EXP2. Improvements in FCR were observed when the sfericase protease was added throughout all ages in EXP1 with a beneficial trend (P<0.067) observed in the cumulative FCR at 42 d in EXP2. The ileal digestible crude protein (IDCP) was significantly higher for birds fed Standard feeds in EXP1 with no other differences in digestibility found in any of the experiments. Protease addition led to improvements in ileal digestibility of dry matter (IDM) and IDCP (P < 0.05) compared to no protease addition in EXP1 as well as in ileal digestibility of energy (IDE) when 30,000 protease units were added. The present report demonstrates that the novel sfericase protease was successful in compensate broiler performance when reductions of 6% digestible Lys and 20 kcal/kg AME were imposed. This compensation, however, seemed more notable when birds were fed diets formulated to support moderate rather than maximum growth and having animal protein in the feed formula.

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“…Exogenous enzymes increase the growth of birds through their ability not only to reduce the adverse effects of anti-nutritional factors (non-starch polysaccharide) but also to decrease cell membrane integrity, changes the gut microflora which consequently improves utilization of protein solubility and energy digestibility (Olukosi et al, 2015;Singh et al, 2017). Several commercially produced enzyme products composed of pure single digestive enzymes (protease, amylase, and lipase) have been used technically to improve nutrient digestibility and growth by augmenting the insufficient quantities of enzymes eRBCA-2022-1673 eRBCA-2022-1673 produced by the animal (Cowieson et al, 2019;Jabbar et al, 2021a). Although, numerous studies have indicated that cocktails of these enzymes deliver more significant improvements in inherent digestibility, such as apparent metabolizable energy (AME), amino acids (AA) digestibility, nutrient availability, and to a lesser extent, reduced nutrient excretion, their result in corn soybean meal (SBM)-based diet has been inconsistent (Romero et al, 2013;2014;Kaczmarek et al, 2014;Adebiyi & Olukosi, 2015).…”

Section: Introductionmentioning

confidence: 99%

Dietary Inclusion of Pancreatin Enzyme on the Ileal and Fecal Digestibility of Nutrients in Layer-Type Cockerels

Sosa-Montes

Ordaz-Contreras

Pró-Martínez³

et al. 2023

Braz. J. Poult. Sci.

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The current experiment aimed to evaluate the effects of Pancreatin supplementation at different levels on ileal and fecal digestibility in layertype cockerels. A total of 480-day-old silver brown Hy-Line male chicks were randomly allocated into 5 treatments, 6 replicates (16 birds per pen) arranged in a completely randomized design. Pancreatin enzyme was supplemented on a basal corn-soybean meal-based diet at 0, 250, 500, 750, and 1000 mg/kg and was fed in two growth phases (starter and grower). The results indicated that at the end of the starter stage, except for 1000 mg/kg, dietary Pancreatin supplementation levels increased (p<0.05) the ileal crude protein (CP). Similarly, addition of Pancreatin increased (p< 0.05) apparent ileal amino acids (AA) digestibility (AIAAD) total means of AA (MTAA), means of indispensable AA (MIAA) and dispensable AA (MDAA) with the optimum performance on 250 mg/kg and 500 mg/kg. However, except for histidine and alanine which were negatively affected (p<0.05), and MIAA, MDAA, MTAA which were also positively affected, the addition level at 1000 mg/kg did not affect most of the AIAAD compared to the non-supplemented. Further, Pancreatin supplementation had no effect (p>0.05) on nitrogen digestibility (ND), nitrogen retention (NR), digestible energy (DE), apparent metabolizable energy (AME), dry matter digestibility (DMD), dry matter retention (DMR), and apparent metabolizable energy corrected for nitrogen (AME-n) on fed starter diet. On the other hand, at the end of the grower stage, dietary Pancreatin enzyme supplementation reduced (p<0.05) the ileal CP, MIAA, MDAA, MTAA, AIAAD, AME, AME-n, ND, NR, DE, DMD, and DMR in a dose-dependent manner. The rate of reduction was more marked on Pancreatin addition level 1000 mg/kg. In conclusion, Pancreatin supplementation at 250 mg/kg, 500 mg/kg, and 750 mg/kg improved AIAAD and ileal CP, especially at the young age. The rate of pancreatin enzyme effect was dependent on enzyme supplement level to the ileal CP and individual amino acid.

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Impact of Microbial Protease Enzyme and Dietary Crude Protein Levels on Growth and Nutrients Digestibility in Broilers over 15–28 Days

Cited by 29 publications

References 20 publications

Potential Applications of Moringa oleifera in Poultry Health and Production as Alternative to Antibiotics: A Review

Potential Applications of Moringa oleifera in Poultry Health and Production as Alternative to Antibiotics: A Review

Growth performance and nutrient digestibility of broiler chickens as affected by a novel protease

Dietary Inclusion of Pancreatin Enzyme on the Ileal and Fecal Digestibility of Nutrients in Layer-Type Cockerels

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