Impact of milking modalities on the occurrence of subclinical mastitis and the anti-bacterial effect of honey on isolated bacteria (Escherichia coli)

Abstract

Prevention of mastitis is a major concern in dairy cattle breeding, and is among the most costly diseases of dairy companies. The purpose of this study was to compare two types of milking practices; one using manual milk-ing and the other using mechanical milking to determine milking type consequences on the occurrence and spread of subclinical mastitis. One hundred dairy cows were brought to the slaughterhouse following a fall in milk production due to subclinical mastitis, of which 64 cows were mechanically milked and 36 cows were manually milked. After anti-mortem and post-mortem examinations, we found that 58% of cows in mechanical milking mode had mastitis. Amongst them 9% of cows had mastitis spread on the four teats. However, the rate was higher (68%) in cows where milking was done manually, of which 49% mastitis spread on the four teats. The bacterio-logical analyses was carried out regarding coliforms, Staphylococci and Streptococci on the raw milks of cows suspected of mastitis, irrespective of the mode of milking.. We chose E. coli and found that its resistance against antibiotics tested was important, except Gentamicin where the sensitivity was average with an inhibition zone of 1.6 cm. We then evaluated the effect of pure honey on the strain to see its antiseptic effect. We noticed the inhibition zone diameters of 2.2 cm. We also tested the effect of diluted honey, the results of which were not satisfactory because of the decreased antisep-tic activity possibly owing to decreased acidity of the honey.

Balın GSBL ve Biyofilm Üreten Enterobacterales Üzerindeki Antibakteriyel Etkisinin Değerlendirilmesi

“…Mastitis is an essential inflammation of the mammary gland that affects milking and dairy production and causes economic losses (Tepeli and Zorba, 2017;Nadia and Kheira, 2018;Krömker et al, 2019). Mastitis occurs in two types as clinical and subclinical.…”

“…Subclinical mastitis infections can be prevented by early diagnosis of the disease, post-milking teat disinfection, and adequate pre-milking hygiene (Das et al, 2017). Antibiotics have been used in the treatment of mastitis for many years (Nadia and Kheira, 2018). The extensive and inappropriate use of antibiotics in dairy farms induces resistance among pathogens in mastitis.…”

“…Moreover, blaCTX-M-1 is the most common genotype in animals according to available studies (EFSA, 2011;Ögedey et al, 2016;Das et al, 2017;Yıldırım and Pehlivanoğlu, 2018). Alternative natural treatment methods such as bee products, phytotherapy, and essential oils were evaluated to reduce the use of antibiotics in the treatment of mastitis (Bal, 2011;Nadia and Kheira, 2018). Honey and bee products were used in folk medicine since ancient times due to biological activities such as antimicrobial, antioxidant, antiinflammatory, immune modulator, and antitumor effects (Kwakman et al, 2008;Cinar, 2020;Kolaylı et al, 2020).…”