Impact of nano-morphology, lattice defects and conductivity on the performance of graphene based electrochemical biosensors

Tite, Teddy; Chiticaru, Elena Alina; Burns, Jorge S.; Ioniță, Mariana

doi:10.1186/s12951-019-0535-6

Cited by 40 publications

(32 citation statements)

References 144 publications

(181 reference statements)

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“…Therefore, serological diagnosis of the dengue IgM antibody is a less reliable test for acute dengue infection. Thus, to increase the detection rate of dengue, irrespective of the disease stage, the developed IgM biosensor should be combined with our previously developed NS1 biosensor [ 16 ] for a concurrent detection of both biomarkers.…”

Section: Discussionmentioning

confidence: 99%

“…The strong chemical inertness of carbon electrodes provides a variety of working potential with low electrical resistivity. They also have a crystalline structure that provides low residual currents and a high signal to noise ratio [ 16 , 17 ]. In addition, the SPCEs electrochemical biosensor has advantages such as low production cost, simplicity, portability, versatility and ease of mass production.…”

Section: Introductionmentioning

confidence: 99%

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Development and Evaluation of an Electrochemical Biosensor for Detection of Dengue-Specific IgM Antibody in Serum Samples

et al. 2020

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Dengue is an arbovirus disease transmitted mainly by Aedes mosquitoes. As dengue shares similar clinical symptoms with other infectious diseases, prompt and accurate diagnosis is pivotal to clinicians’ decisions on appropriate management. Conventional diagnostic tests to detect the dengue-specific IgM antibody are limited in their performance and ease of use. To address these issues, we developed and evaluated a biosensor based on screen-printed carbon electrodes (SPCEs) for the detection of dengue-specific immunoglobulin M (IgM) antibodies. Various optimisations were performed in order to increase the sensitivity and specificity of the biosensor. For optimal and proper orientation of the paratope sites of goat anti-human IgM capture antibodies (GAHICA), various antibody techniques, including passive, covalent, protein A, protein G and streptavidin/biotin systems, were tested on the SPCEs. The assay reagents for the biosensor were also optimised prior to its evaluation. Analytical sensitivity evaluation was carried out using pooled sera, while analytical specificity evaluation was conducted on a panel of six non-dengue serum samples. Subsequently, diagnostic sensitivity and specificity evaluation were performed using 144 reference samples. Electrochemical current signals generated from H2O2 catalysed by HRP-labelled anti-dengue detection antibodies were measured using the chronoamperometric technique. With a limit of detection (LOD) of 106 serum dilution, the analytical sensitivity of the developed biosensor was 10 times higher than commercial ELISA. The analytical specificity of this dengue IgM biosensor was 100%. Similarly, the biosensor’s diagnostic performance was 100% for sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV). These findings suggest that the developed biosensor has a great potential to be used to diagnose dengue after seroconversion.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and Evaluation of an Electrochemical Biosensor for Detection of Dengue-Specific IgM Antibody in Serum Samples

et al. 2020

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“…The formation of defects and the incorporation of some dopants and functional groups in the electrode material increases the density of electronic states which subsequently helps to promote the electron transfer movement in the electrode/electrolyte interface. [ 36 ] Therefore, a perfect electroactive material should have a balance between the electrical conductivity and the defect density. Furthermore, the effective surface area and porosity of an electrode material is a very crucial factor in governing the electrochemical response of a sensing system.…”

Section: Functional Electroactive and Photoactive Materialsmentioning

confidence: 99%

Advanced Functional Electroactive and Photoactive Materials for Monitoring the Environmental Pollutants

Yan

Kannan

Doonyapisut

et al. 2020

Adv Funct Materials

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Functional materials with attractive electronic and photoelectronic properties show great promise in various fields. In recent decades, tremendous research efforts have been devoted to the design of photoactive and electroactive materials for qualitative and quantitative analysis of environmental pollutants. This review gives a concise overview on the fundamentals and recent research progress of functional material‐based electrochemical and photoelectrochemical technology for environmental pollutant monitoring. The rational design of functional photoactive and electroactive materials with promoted electrochemical properties and basic signaling strategies for electrochemical and photoelectrochemical sensors are presented. Based on these insights, very recent achievements for electrochemical and photoelectrochemical environmental pollutant sensors are summarized from the viewpoint of various functional materials. At last, critical challenges and future research perspectives in this field are proposed and discussed to provide a backdrop for future research.

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“…A variety of approaches have been exploited, including electrochemical biosensors [ 3 – 5 ], fluorescent biosensors [ 6 ], colorimetric biosensors [ 7 , 8 ], potentiometric biosensors [ 9 , 10 ], optical biosensors [ 11 ], and Raman spectroscopy-based platforms [ 12 , 13 ]. Compared with other detection methods, electrochemistry biosensing platforms provide a more facile, cost-effective and a highly sensitive detection method which enables the fast response-recovery times, monitoring different analytes, and a very low detection limit [ 14 – 16 ]. Recent efforts have focused on improving the sensing features of electrochemical biosensors by increasing the specific surface area of the transducers (interacting materials with the target analyte), where the larger the surface area of the sensing materials, the higher their ability to interact with the medium (analytes) [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Graphene impregnated electrospun nanofiber sensing materials: a comprehensive overview on bridging laboratory set-up to industry

2020

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Owing to the unique structural characteristics as well as outstanding physio-chemical and electrical properties, graphene enables significant enhancement with the performance of electrospun nanofibers, leading to the generation of promising applications in electrospun-mediated sensor technologies. Electrospinning is a simple, cost-effective, and versatile technique relying on electrostatic repulsion between the surface charges to continuously synthesize various scalable assemblies from a wide array of raw materials with diameters down to few nanometers. Recently, electrospun nanocomposites have emerged as promising substrates with a great potential for constructing nanoscale biosensors due to their exceptional functional characteristics such as complex pore structures, high surface area, high catalytic and electron transfer, controllable surface conformation and modification, superior electric conductivity and unique mat structure. This review comprehends graphene-based nanomaterials (GNMs) (graphene, graphene oxide (GO), reduced GO and graphene quantum dots) impregnated electrospun polymer composites for the electro-device developments, which bridges the laboratory setup to the industry. Different techniques in the base polymers (preprocessing methods) and surface modification methods (post-processing methods) to impregnate GNMs within electrospun polymer nanofibers are critically discussed. The performance and the usage as the electrochemical biosensors for the detection of wide range analytes are further elaborated. This overview catches a great interest and inspires various new opportunities across a wide range of disciplines and designs of miniaturized point-of-care devices.

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Impact of nano-morphology, lattice defects and conductivity on the performance of graphene based electrochemical biosensors

Cited by 40 publications

References 144 publications

Development and Evaluation of an Electrochemical Biosensor for Detection of Dengue-Specific IgM Antibody in Serum Samples

Development and Evaluation of an Electrochemical Biosensor for Detection of Dengue-Specific IgM Antibody in Serum Samples

Advanced Functional Electroactive and Photoactive Materials for Monitoring the Environmental Pollutants

Graphene impregnated electrospun nanofiber sensing materials: a comprehensive overview on bridging laboratory set-up to industry

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