Impact of Ocimum basilicum leaves powder on immune response of chicken vaccinated against Newcastle Disease Virus

Abstract

In this research, we study the effect of adding the Ocimum basilicum leaves powder (OBLP), as broilers chicken feed additive and study its effects on the innate and adaptive immune responses as well as the antioxidant effects in broilers vaccinated with Newcastle disease virus (NDV) vaccine. A total of 100 one-day old broiler were randomly divided into 4 equal groups, the first group (G1) was served as normal control group, the second group (G2) was served as vaccination control group. Groups 3 (G3) and 4 (G4) fed 0.5% and 1% OBLP in diets, respectively, and vaccinated against NDV. The obtained results showed that the adding of OBLP to diets improved the broilers innate immune response which resulted in significant increases in peripheral blood monocyte cells phagocytic activity, lysozyme levels and lymphoid organs / body weight ratios. Adaptive immune response has also been improved as the hemagglutination inhibition (HI) antibody titers against NDV vaccine in G3 and G4 reached the peak earlier, declined slowly and gave significantly higher titer in G4 than other groups at the end of the experiment. The antioxidant effects of OBLP were evident particularly by using high concentration of OBLP as the serum catalase enzyme (CAT) levels were significantly increased whereas malondialdehyde (MDA) were significantly reduced. It could be concluded that the use of OBLP as a feed additive can boost the innate and adaptive immune response to NDV vaccination and improve the antioxidant activity of broilers in a dose dependent manner.

Estragole: A review of its pharmacology, effect on animal health and performance, toxicology, and market regulatory issues

“…The effectiveness of Estragole against virus infection was reported by Farida and Abdulaziz (59). They found that supplementation of O. basilicum seeds in broiler diets significantly increased the serum hemagglutination inhibition antibody titers and eliminated Infectious Bronchitis Viruses (IBV) and Newcastle Disease (ND).…”

“…According to Farida and Nahid (59), adding Ocimum basilicum leaves powder to broiler chicken feed improves the innate immune response, as seen by considerable improvements in cells' phagocytic activity of peripheral blood monocyte, lymphoid organs, and lysozyme concentrations. According to ELnaggar and El-Tahawy (60), Adding Ocimum basilicum leaves powder or seed oils to the broiler basal diet boosted the phagocytic index and activity of the macrophages, allowing it to be administered effectively as a natural growth promoter to improve broiler chick development and immunological response.…”

Effect of Ocimum basilicum in Cobb 500 chickens on the productive and bacterial parameters

“…La adición de O. basilicum a la ración de pollos de engorde tiene efectos beneficiosos sobre la respuesta inmune innata que se refleja como aumentos significativos en monocitos de sangre periférica, actividad fagocítica, niveles de lisozima y órganos linfoides proporciones de peso corporal especialmente cuando se usa al 1%, pero si se usa al 0,5% podría ser más ventajoso y podría usarse en áreas con un alto riesgo de infección viral, ya que ayudará en maximizando los beneficios de las vacunas (Mohamed y Abd, 2020).…”