Impact of omega-3 fatty acids on expression of angiogenic cytokines and angiogenesis by adipose-derived stem cells

Parshyna, Iryna; Lehmann, Susann; Grahl, Katrin; Pahlke, Claudia; Frenzel, Annika; Weidlich, Helmut H.; Morawietz, Henning

doi:10.1016/j.atherosclerosissup.2017.05.040

Cited by 8 publications

(9 citation statements)

References 28 publications

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“…13,14 By releasing a series of multifunctional effect factors, ADSCs are promising candidates to prepare a favorable microenvironment for the regeneration of injured tissues, thereby facilitating angiogenesis, extracellular matrix remodeling, and re-epithelialization in cutaneous wound healing. 15–17 In this study, we demonstrated that genetic deletion of E2F1 enhanced the secretory factor of ADSCs by up-regulating VEGF and TGF-β1 expression to promote wound healing, suggesting an effective strategy to enhance the therapeutic bioactivity of ADSCs in wound repair.…”

Section: Discussionmentioning

confidence: 77%

E2F1- deficient adipose-derived stem cells improve skin wound closure in mice by upregulating paracrine expression of VEGF and TGF-β1

Zhang

et al. 2023

Plastic &Amp; Reconstructive Surgery

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Background: Wound healing is a widespread health problem that imposes a financial burden on health systems. Cell therapy with genetically modified adipose-derived stem cells (ADSCs) is a promising strategy for dysregulated wound repair. E2F transcription factor 1 (E2F1) is a bidirectional regulator of cytokines. Here, the authors aimed to investigate the impact and potential mechanism of E2F1–/– ADSCs in promoting the wound healing process. Methods: Forty-five C57BL/6 mice (specific pathogen-free, male) with 10-mm full-thickness wounds were randomly treated with subcutaneous injection of 2 × 106 wild-type ADSCs, 2 × 106 E2F1–/– ADSCs, or phosphate-buffered saline. The wound closure rate was monitored at days 0, 3, 7, 10, and 14 after treatment. The collagen synthesis, angiogenesis, and wound contraction were calculated by Masson, immunohistochemistry, and immunofluorescent staining (CD31 and KI67), Western blotting (α-smooth muscle actin, collagen I, vascular endothelial growth factor, and transforming growth factor–β1) separately at day 14. In vitro, the conditioned media (CM) of wild-type ADSCs and E2F1–/– ADSCs were collected to evaluate the impact on proliferation, migration, and angiogenesis. Results: In vivo, the E2F1–/– ADSC group exhibited increased healing rate, proliferating vessels, and collagen synthesis compared with control at day 14 (P < 0.05). Moreover, E2F1–/– ADSCs showed enhanced vascular endothelial growth factor and transforming growth factor–β1 expression in the wound site and CM, and the CM from E2F1–/– ADSCs promoted the proliferation, migration, and tube formation of co-cultured cells in vitro (P < 0.05). Conclusion: The E2F1–/– ADSCs exhibited a strong paracrine ability to improve the vascularization process and collagen deposition, thereby accelerating wound healing in the rodent model. Clinical Relevance Statement: These findings show that targeting transcription factor E2F1 could regulate the paracrine function of ADSCs, developing E2F1-modified ADSCs as an effective therapeutic option for wound healing and regeneration.

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Section: Discussionmentioning

confidence: 77%

E2F1- deficient adipose-derived stem cells improve skin wound closure in mice by upregulating paracrine expression of VEGF and TGF-β1

Zhang

et al. 2023

Plastic &Amp; Reconstructive Surgery

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“…Due to the same angiogenic capacity of the 3D‐ASCs, Parshyna et al studied the role of several omega‐3 fatty acids. In particular, docosahexaenoic acid promoted the sprouting activity of ASCs supporting the formation of microvascular networks in cocultures with HUVECs into a collagen matrix (Parshyna et al, 2017 ).…”

Section: Resultsmentioning

confidence: 99%

Systematic review on spheroids from adipose‐derived stem cells: Spontaneous or artefact state?

Stefano

Urrata

Trapani

et al. 2022

Journal Cellular Physiology

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Three‐dimensional (3D) cell cultures represent the spontaneous state of stem cells with specific gene and protein molecular expression that are more alike the in vivo condition. In vitro two‐dimensional (2D) cell adhesion cultures are still commonly employed for various cellular studies such as movement, proliferation and differentiation phenomena; this procedure is standardized and amply used in laboratories, however their representing the original tissue has recently been subject to questioning. Cell cultures in 2D require a support/substrate (flasks, multiwells, etc.) and use of fetal bovine serum as an adjuvant that stimulates adhesion that most likely leads to cellular aging. A 3D environment stimulates cells to grow in suspended aggregates that are defined as “spheroids.” In particular, adipose stem cells (ASCs) are traditionally observed in adhesion conditions, but a recent and vast literature offers many strategies that obtain 3D cell spheroids. These cells seem to possess a greater ability in maintaining their stemness and differentiate towards all mesenchymal lineages, as demonstrated in in vitro and in vivo studies compared to adhesion cultures. To date, standardized procedures that form ASC spheroids have not yet been established. This systematic review carries out an in‐depth analysis of the 76 articles produced over the past 10 years and discusses the similarities and differences in materials, techniques, and purposes to standardize the methods aimed at obtaining ASC spheroids as already described for 2D cultures.

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“…It is suggested that highly purified SVFG combined with APRFE can significantly promote the angiogenesis of injured tissue and accelerate vascular reconstruction. Because angiogenesis is an important part of radiation injury repair, the injectable highly purified SVFG enriched with ADSCs have been confirmed to differentiate into vascular endothelial cells, smooth muscle cells, and pericytes, or directly participating in angiogenesis (25)(26)(27). Moreover, cytokines secreted by ADSCs and PDGF, VEGF, FGF-2, EGF, IGF and TGF-β in APRFE effectively promote neovascularization (28).…”

Section: Discussionmentioning

confidence: 99%

“…Twelve rabbits were randomly divided into four groups, and chloral hydrate was injected into each group according to 400 mg/kg. After anesthesia, different dose (15,20,25,30 Gy) was irradiated according to the dose of 6 MeV electron beam in each group (dose rate was 300 mu/min × 12 min = 3,500 mu). After 24 hours of irradiation, pictures were taken, the healing rate of each group was recorded to select the best radiation dose.…”

Section: Establishing Model Of Irradiated Skin and Soft Tissue Injury...mentioning

confidence: 99%

Promoting repair of highly purified stromal vascular fraction gel combined with advanced platelet-rich fibrin extract for irradiated skin and soft tissue injury

Li¹,

Gan²,

Liang³

et al. 2022

Ann Transl Med

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Background To evaluate the effect of highly purified stromal vascular fraction gel (SVFG) combined with advanced platelet-rich fibrin extract (APRFE) in treatment of irradiated skin and soft tissue injury. Methods The subcutaneous fat and whole blood of 4 rabbits were collected to isolate the SVFG and APRFE, respectively. Forty-eight rabbits were divided into 4 groups to prepare irradiated skin injury models with 25 Gy for 24 hours; corresponding dose were performed subcutaneously injected into wounds. In group A, the rabbits were treated with 0.3 mL APRFE combined with 1 mL SVFG. In group B, the rabbits were treated with 1 mL SVFG. In group C, the rabbits were treated with 0.3 mL APRFE, and group D was treated with 1 mL normal saline. The wound healing was detected on the 2, 5, 9 and 14 d after intervention. The wounds tissue was cut for hematoxylin and eosin (HE) staining to observe the structure and Masson staining to observe the collagen content. The expression of CD31 in each group was detected by immunohistochemistry (IHC), the protein and mRNA levels of K19, hypoxia inducible factor-1 alpha (HIF-1α), vascular endothelial growth factor (VEGF), interleukin 8 (IL-8) and interleukin 10 (IL-10) were detected respectively by Western blot (WB) and reverse transcription-polymerase chain reaction (RT-PCR) on 7, 14 and 28 d after intervention. Results It is revealed that wound healing rates from 5 to 14 d in group A was significantly higher than that of control. The wounds healing rates in group B and C were significantly higher than that of control after 12 d. Masson staining results showed that the collagen content in group A was significantly higher than that of the other 3 groups on the 7, 14 and 28 d. The results of IHC showed that the expression of CD31 in group A was significantly higher than that of the other 3 groups on 7, 14 and 28 d. WB and RT-PCR results showed that relative expression levels of K19, HIF-1α, VEGF, IL-10 in group A were significantly higher than that of the other 3 groups on 7, 14 and 28 d. However, the relative expression levels of IL-8 in group A was significantly lower than that of the other 3 groups on 7, 14 and 28 d. Conclusions SVFG combined with APRFE can promote the repair of irradiated skin and soft tissue injury by accelerating angiogenesis, promoting collagen synthesis and reducing inflammation.

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Impact of omega-3 fatty acids on expression of angiogenic cytokines and angiogenesis by adipose-derived stem cells

Cited by 8 publications

References 28 publications

E2F1- deficient adipose-derived stem cells improve skin wound closure in mice by upregulating paracrine expression of VEGF and TGF-β1

E2F1- deficient adipose-derived stem cells improve skin wound closure in mice by upregulating paracrine expression of VEGF and TGF-β1

Systematic review on spheroids from adipose‐derived stem cells: Spontaneous or artefact state?

Promoting repair of highly purified stromal vascular fraction gel combined with advanced platelet-rich fibrin extract for irradiated skin and soft tissue injury

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