Impact of oocyte cryopreservation on embryo development

Magli, M. Cristina; Lappi, Michela; Ferraretti, Anna Pia; Capoti, Alessandra; Ruberti, Alessandra; Gianaroli, Luca

doi:10.1016/j.fertnstert.2009.01.148

Cited by 37 publications

(19 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The survival rate achieved in the present study is similar to that previously observed with the 0.3 M sucrose method [7,15,16,[31][32][33][34][35][36][37] and the 0.2 M sucrose method [17,19,21]. In some of the above studies only oocytes with optimal morphology were cryopreserved [37,38] which is not the case in the present study.…”

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

Gook

Edgar

2011

J Assist Reprod Genet

View full text Add to dashboard Cite

Previous reports of slow cooling of human mature oocytes have shown a reduced clinical efficiency relative to fresh oocytes. This study reports that equivalent fertilization and implantation rates to those obtained using fresh oocytes and cryopreserved embryos can be achieved with human mature oocytes dehydrated in 1.5 M propanediol and 0.2 M sucrose at 37°C and cryopreserved using slow cooling rates.

show abstract

Section: Discussionsupporting

confidence: 91%

“…In some of the above studies only oocytes with optimal morphology were cryopreserved [37,38] which is not the case in the present study.…”

Section: Discussionmentioning

confidence: 71%

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

Gook

Edgar

2011

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Vitrification gained a foothold only after 2005, prior to which only ten human pregnancies resulting from vitrified oocytes were reported (Oktay et al 2006). Although high oocyte survival rate is achieved with both methods, fertilization and ET rates are still considerably lower than when fresh oocytes are used (Magli et al 2010). When comparing slow freezing to vitrification, higher oocyte survival rates are achieved by the latter (95%, 899/948 vs 75%, 1275/1683 respectively), but pregnancy rate per thawed/warmed oocyte is still low -in the range of 1.9-8.6% for slow freezing and 3.9-18.8% for vitrification (Chen & Yang 2009).…”

Section: Overcoming the Difficultiesmentioning

confidence: 99%

Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification

2011

View full text Add to dashboard Cite

Preservation of female genetics is currently done primarily by means of oocyte and embryo cryopreservation. The field has seen much progress during its four-decade history, progress driven predominantly by research in humans, cows, and mice. Two basic cryopreservation techniques rule the field -controlled-rate freezing, the first to be developed, and vitrification, which, in recent years, has gained a foothold. While much progress has been achieved in human medicine, the cattle industry, and in laboratory animals, this is far from being the case for most other mammals and even less so for other vertebrates. The major strides and obstacles in human and other vertebrate oocyte and embryo cryopreservation will be reviewed here.

show abstract

“…While the success rates of IVF with oocytes that have been subjected to slow freezing are significantly lower than those with fresh oocytes (Borini et al, 2010;Magli et al, 2010), cryopreservation with the vitrification technique offers success rates similar to those with fresh 4 oocytes (Almodin et al, 2010;Grifo and Noyes, 2010;Kim et al, 2010;Rienzi et al, 2010;Trokoudes et al, 2011). However, just as for fresh oocytes, the outcomes of IVF with vitrified oocytes areunsurprisingly -highly dependent on maternal age at the time of freezing (Rienzi and Ubaldi, 2011).…”

Section: Introductionmentioning

confidence: 99%

Social egg freezing: for better, not for worse

Mertes

Pennings

2011

Reproductive BioMedicine Online

103

View full text Add to dashboard Cite

The possibility for healthy women to cryopreserve their oocytes in order to counter future infertility has gained momentum in recent years. However, women tend to cryopreserve oocytes at an age that is suboptimal from a clinical point of view -in their late thirties -when both oocyte quantity and quality have already considerably diminished and success rates for eventually establishing a pregnancy are thus limited. This also gives rise to ethical concerns, as the procedure is seen as giving false hope to (reproductively speaking) older women. We evaluate which measures can be taken to turn social freezing into a procedure that is both clinically and ethically better than the current practice. The main objective of these measures is to convince those women who are most likely to (want to) reproduce at an above average age to cryopreserve their oocytes at a time when this intervention is still likely to lead to a life birth and to discourage fertility clinics from specifically targeting women who have already surpassed the age at which good results can be expected.

show abstract

Impact of oocyte cryopreservation on embryo development

Cited by 37 publications

References 43 publications

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification

Social egg freezing: for better, not for worse

Contact Info

Product

Resources

About