Impact of PCOS on early embryo cleavage kinetics

Wissing, Marie Louise Muff; Bjerge, Marianne Rich; Olesen, A.I.G.; Hoest, T.; Mikkelsen, Anne Lis

doi:10.1016/j.rbmo.2013.11.017

Cited by 86 publications

(68 citation statements)

References 35 publications

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“…Oocyte quality after COH in PCOM patients. Fertil Steril 2014. endometrial receptivity (6,34,35). Furthermore, our delivery rate in the present study is close to our pregnancy rate, confirming that pregnancies in patients with PCOM without metabolic disorders have the same prognosis as in controls.…”

Section: Discussionsupporting

confidence: 88%

Is polycystic ovarian morphology related to a poor oocyte quality after controlled ovarian hyperstimulation for intracytoplasmic sperm injection? Results from a prospective, comparative study

Sigala

Sifer

Dewailly

et al. 2015

Fertility and Sterility

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Section: Discussionsupporting

confidence: 88%

Is polycystic ovarian morphology related to a poor oocyte quality after controlled ovarian hyperstimulation for intracytoplasmic sperm injection? Results from a prospective, comparative study

Sigala

Sifer

Dewailly

et al. 2015

Fertility and Sterility

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“…The present study demonstrated that women with PCOS had lower serum FSH [18]. Embryos from PCOS women were found to have a slower development during the blastocyst stage [17]. In this study, we constructed the insulin intervention pregnant mouse model on D4.…”

Section: Discussionmentioning

confidence: 99%

“…Women with PCOS or T2DM are reported to have ovarian dysfunction, low implantation rates, slow development, and high miscarriage rates [1,[17][18][19]. However, the underlying mechanism of this phenomenon is still unknown.…”

Section: Animal Model and Tissue Collectionmentioning

confidence: 99%

High insulin impaired ovarian function in early pregnant mice and the role of autophagy in this process

et al. 2017

Endocr J

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METABOLIC DISORDERS, such as PCOS (polycystic ovary syndrome) and T2DM (type 2 diabetes mellitus), are reported to be associated with high miscarriage rates, decreased pregnancy rates, and low live birth rates [1]. PCOS and T2DM are characterized by hyperinsulinemia [2]. PCOS is an endocrine and metabolic disorder with a prevalence ranging from 5 to 13% in women of reproductive age. Moreover, PCOS is often accompanied by menstrual dysfunction, anovulaHigh insulin impaired ovarian function in early pregnant mice and the role of autophagy in this process Abstract. Metabolic disorders, such as PCOS (polycystic ovarian syndrome) and T2DM (type 2 diabetes mellitus), are associated with menstrual dysfunction, anovulation, infertility, and early pregnancy loss. Ovarian dysfunction is not only related to low pregnancy rates but also to the increased risk of miscarriage. Women with PCOS or T2DM, characterized by hyperinsulinemia, commonly experience ovarian dysfunction. In this study, we first explored whether high insulin levels directly affected ovarian functioning during embryo implantation. Mice in the insulin-treated group were given a subcutaneous injection of human recombinant insulin. After insulin treatment, serum levels of E2 (estrogen), PROG (progesterone), LH (luteinizing hormone), and FSH (follicle-stimulating hormone) were obviously lower, and there was a significant decrement of ovarian GDF9 (growth differentiation factor 9) mRNA. H&E (hematoxylin and eosin) staining showed a greater number of immature follicles and less luteinization in the insulin group. Further autophagy was studied in this process. A significant increase of P62 (SQSTM1/Sequestosome1) and a decrease of Cathepsin B, BECN1 (Beclin 1), and ULK1 (Unc-51-like kinase 1) mRNA in ovary was found in the insulin group. Western blot analysis showed that the expressions of LC3 (microtubule-associated protein 1 light chain 3), BECN1, and Cathepsin B proteins in ovaries from insulin group were obviously reduced, while P62 proteins were significantly increased. All these results illustrated that insulin could directly impair ovarian function during embryo implantation and the imbalance of ovarian autophagy due to insulin. Autophagy could enhance the impaired ovarian function results from insulin.

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“…However, evidence does exist to show external factors may alter embryo morphokinetics, and such factors may originate from either the patient population [27][28][29], ovarian stimulation [24][25][26], or laboratory culture conditions [30,31]. In the present study, the two participating laboratories' datasets had significantly different cycle characteristics (Table 1) and embryo culture conditions (Table 2), which may have contributed to the significant differences in morphokinetics that were found (Table 3).…”

Section: Discussionmentioning

confidence: 69%

“…It must also be noted that the growth of embryos is not constant, and may be influenced by external factors. Such confounding factors have been associated with the patient (e.g., the stimulation regimen used for ovarian stimulation [24,25], gonadotropin dose and hormonal levels [24,26], smoking [27], the presence of hyperandrogenic polycystic ovarian syndrome in the female [28], and sperm DNA fragmentation in the male [29]) and also the culture system (e.g., oxygen concentration in the incubator [30] and the use of different culture media formulations [31]). …”

Section: Introductionmentioning

confidence: 99%

Assessment of human embryos by time-lapse videography: A comparison of quantitative and qualitative measures between two independent laboratories

Liu

Copeland²,

Stevens³

et al. 2015

Reproductive Biology

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Impact of PCOS on early embryo cleavage kinetics

Cited by 86 publications

References 35 publications

Is polycystic ovarian morphology related to a poor oocyte quality after controlled ovarian hyperstimulation for intracytoplasmic sperm injection? Results from a prospective, comparative study

Is polycystic ovarian morphology related to a poor oocyte quality after controlled ovarian hyperstimulation for intracytoplasmic sperm injection? Results from a prospective, comparative study

High insulin impaired ovarian function in early pregnant mice and the role of autophagy in this process

Assessment of human embryos by time-lapse videography: A comparison of quantitative and qualitative measures between two independent laboratories

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