Impact of Pesticides Used in the Culture of the Vine on the Viability of the Yeast Saccharomyces Cerevisiae Wine in Chronological Aging

Owsiak, Alina; Marchel, Magdalena; Żyracka, Ewa

doi:10.15414/jmbfs.2015.4.special1.48-51

Cited by 4 publications

(1 citation statement)

References 17 publications

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“…For example, the early embryonic development of amphibians was seriously affected by the widespread use of paraquat, which could induce ROS generation. The processes related to cell aging were intensely affected by adding pesticides in the culture of yeast Saccharomyces cerevisiae [ 64 ], likely because pesticides induce oxidative lesions by stimulating the production of free radicals. UV irradiation provokes ROS formation, leading to the activation of complex signalling pathways, such as mitogen activated protein kinase (MAPK) and nuclear factor kappa-β (NF-кβ) pathways, finally causing cellular death [ 4 , 65 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Decapentapletic Gene (AccDpp) from Apis cerana cerana and Its Possible Involvement in Development and Response to Oxidative Stress

Zhao

Wang

et al. 2016

PLoS ONE

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To tolerate many acute and chronic oxidative stress-producing agents that exist in the environment, organisms have evolved many classes of signal transduction pathways, including the transforming growth factor β (TGFβ) signal pathway. Decapentapletic gene (Dpp) belongs to the TGFβ superfamily, and studies on Dpp have mainly focused on its role in the regulation of development. No study has investigated the response of Dpp to oxidative pressure in any organism, including Apis cerana cerana (A. cerana cerana). In this study, we identified a Dpp gene from A. cerana cerana named AccDpp. The 5΄ flanking region of AccDpp had many transcription factor binding sites that relevant to development and stress response. AccDpp was expressed at all stages of A. cerana cerana, with its highest expression in 15-day worker bees. The mRNA level of AccDpp was higher in the poison gland and midgut than other tissues. Furthermore, the transcription of AccDpp could be repressed by 4°C and UV, but induced by other treatments, according to our qRT-PCR analysis. It is worth noting that the expression level of AccDpp protein was increased after a certain time when A. cerana cerana was subjected to all simulative oxidative stresses, a finding that was not completely consistent with the result from qRT-PCR. It is interesting that recombinant AccDpp restrained the growth of Escherichia coli, a function that might account for the role of the antimicrobial peptides of AccDpp. In conclusion, these results provide evidence that AccDpp might be implicated in the regulation of development and the response of oxidative pressure. The findings may lay a theoretical foundation for further genetic studies of Dpp.

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Section: Discussionmentioning

confidence: 99%

Characterization of a Decapentapletic Gene (AccDpp) from Apis cerana cerana and Its Possible Involvement in Development and Response to Oxidative Stress

Zhao

Wang

et al. 2016

PLoS ONE

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Alcoholic Fermentation as a Strategy to Mitigate Pesticides and Mycotoxins

Giacomini

Acosta

Cerqueira

et al. 2023

Food Bioprocess Technol

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In this study alcoholic fermentation was explored to reduce the pesticides -2,4-dichlorophenoxyacetic acid (2,4-D) and procymidone -and the mycotoxin ochratoxin A (OTA), besides evaluating their effects on quality parameters of the process. Fermentation (at 20°C for 168 h) was conducted in synthetic must (YPD and 10 g hL − 1 of yeast Saccharomyces cerevisiae) to which the following were added: 2,4-D (6.73 mg L − 1 ) and procymidone (2.24 mg L − 1 ), simultaneously (Treatment 1); and OTA (0.83 and 2.66 µg L − 1 ) (Treatments 2 and 3). The control treatment had no contaminants. All contaminated treatments showed lower ethanol production and cell viability.The highest decrease in contaminants was found after 168 h of fermentation: 22 and 65% in the cases of both pesticides while OTA decreased 52 and 58% (Treatments 2-0.83 µg L − 1 and 3-2.66 µg L − 1 ). Decrease in procymidone and OTA due to metabolic processes in yeast cells is mainly related to peroxidase activity and glutathione production.

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Molecular and functional characaterization of the novel odorant-binding protein geneAccOBP10fromApis cerana cerana

Guo

Hao

Cui

et al. 2020

The Journal of Biochemistry

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Summary Odorant-binding proteins (OBPs) play an important role in odor perception and transport in insects. However, little is known about whether OBPs perform other functions in insects, particularly in Apis cerana cerana. Within this study, an OBP gene (AccOBP10) was isolated and identified from Apis cerana cerana. Both homology and phylogenetic relationship analyses indicated that the amino acid sequence of AccOBP10 had a high degree of sequence identity with other members of the gene family. Analysis of real-time quantitative PCR (qRT-PCR) showed that AccOBP10 mRNA was expressed at higher levels in the venom gland than in other tissues. The mRNA transcript expression of AccOBP10 was upregulated by low temperature (4 °C), hydrogen peroxide (H2O2), pyridaben, methomyl and imidacloprid but downregulated by heat (42 °C), ultraviolet (UV) light, vitamin C, mercuric chloride (HgCl2), cadmium chloride (CdCl2), paraquat and phoxim. Expression of AccOBP10 under abiotic stress was analyzed by Western blotting, and the results were consistent with those of qRT-PCR. And as a further study of AccOBP10 function, we demonstrated that knockdown of AccOBP10 by RNA interference (RNAi) could slightly increase the expression levels of some stress-related genes. Collectively, these results suggest that AccOBP10 is mainly involved in the response to stress conditions.

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Impact of Pesticides Used in the Culture of the Vine on the Viability of the Yeast Saccharomyces Cerevisiae Wine in Chronological Aging

Cited by 4 publications

References 17 publications

Characterization of a Decapentapletic Gene (AccDpp) from Apis cerana cerana and Its Possible Involvement in Development and Response to Oxidative Stress

Characterization of a Decapentapletic Gene (AccDpp) from Apis cerana cerana and Its Possible Involvement in Development and Response to Oxidative Stress

Alcoholic Fermentation as a Strategy to Mitigate Pesticides and Mycotoxins

Molecular and functional characaterization of the novel odorant-binding protein geneAccOBP10fromApis cerana cerana

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