Impact of pneumoperitoneum on tumor growth

Lécuru, Fabrice; Agostini, Aubert; Camatte, S.; Robin, F.; Aggerbeck, Μartine; Jaı̈s, Jean-Philippe; Vilde, F; Taurelle, R

doi:10.1007/s00464-001-9226-z

Cited by 29 publications

(17 citation statements)

References 23 publications

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“…However, the occurrence of port-site metastasis and peritoneal metastasis reported in early studies of laparoscopic surgery for cancer led to many studies investigating the biologic effects of positive pressure pneumoperitoneum on tumor growth and the development of trocar recurrences. Several experimental studies suggested that laparoscopic surgery can promote tumor growth [6,19] . Some animal studies have also shown that CO2, especially at high insufflation pressures increased the incidence of distant metastases [20,21] .…”

Section: Discussionmentioning

confidence: 99%

“…Recently, several studies demonstrated that the five-year sur vival rates were similar after laparoscopically assisted colectomy and open colectomy for cancer patients, suggesting that the laparoscopic approach is an acceptable alternative to open surgery for colorectal cancer [3][4][5] . However, whether CO2 insufflation, which is widely used in laparoscopic surgery, increases the metastatic potential of tumor cells is still the focus of related studies [6][7][8] .…”

Section: Introductionmentioning

confidence: 99%

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Higher CO₂-insufflation pressure inhibits the expression of adhesion molecules and the invasion potential of colon cancer cells

Ma¹,

Feng²,

Zhang³

et al. 2009

WJG

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Author contributions: Ma JJ carried out the Adhesion assays, drafted the manuscript and participated in the design of the study; Feng B carried out the molecular expression studies, and in vitro pneumoperitoneum establishment; Zhang Y carried out the cell invasive assay; Li JW participate in the induction of intra-abdominal tumors; Lu AG participated in the molecular expression studies; Wang ML performed the statistical analysis; Peng YF, Hu WG, Yue F participated in the cell invasive assay; Zheng MH conceived of the study, and participated in its design and coordination and helped to draft the manuscript. METHODS:With an in vitro artificial pneumoperitoneum model, SW1116 human colon carcinoma cells were exposed to CO2-insufflation in 5 different pressure groups: 6 mmHg, 9 mmHg, 12 mmHg, 15 mmHg and control group, respectively for 1 h. Expression of E-cadherin, ICAM-1, CD44 and E-selectin was measured at 0, 12, 24, 48 and 72 h after CO2-insufflation using flow cytometry. The adhesion and invasion capacity of SW1116 cells before and after exposure to CO2-insufflation was detected by cell adhesion/invasion assay in vitro. Each group of cells was injected intraperitoneally into 16 BALB/C mice. The number of visible abdominal cavity tumor nodules, visceral metastases and survival of the mice were recorded in each group. RESULTS:The expression of E-cadherin, ICAM-1, CD44 and E-selectin in SW1116 cells were changed significantly following exposure to CO2 insufflation at different pressures (P < 0.05). The expression of E-cadherin, CD44 and ICAM-1 decreased with increasing CO2-insufflation pressure. The adhesive/ invasive cells also decreased gradually with increasing pressure as determined by the adhesion/invasion assay. In animal experiments, the number of abdominal cavity tumor nodules in the 15 mmHg group was also significantly lower than that in the 6 mmHg group (29.7 ± 9.91 vs 41.7 ± 14.90, P = 0.046). However, the survival in each group was not statistically different. CONCLUSION: CO2-insufflation

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Higher CO₂-insufflation pressure inhibits the expression of adhesion molecules and the invasion potential of colon cancer cells

Ma¹,

Feng²,

Zhang³

et al. 2009

WJG

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“…Whether CO 2 pneumoperitoneum will promote trocar implantation and extensive abdominal metas- tasis after laparoscopic tumor operation was widely discussed in recent ten years, but there were still no conclusions (Zhang and Kong, 2004;Leister et al, 2003;Yavuz et al, 2003 (Hirabayashi et al, 2002;Lecuru et al, 2002). But these factors could not explain why He pneumoperitoneum would not promote trocar implantation (Neuhaus et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Influence of CO₂pneumoperitoneum on intracellular pH and signal transduction in cancer cells

Cao¹,

Ding²,

Que³

et al. 2005

J. Zhejiang Univ. Sci.

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Abstract:Object: The authors studied the influence of CO 2 pneumoperitoneum on intracellular pH and signal transduction arising from cancer cell multiplication in laparoscopic tumor operation. Method: They set up a simulation of pneumoperitoneum under different CO 2 pressure, and then measured the variation of intracellular pH (pHi) at different time and the activity of protein kinase C (PKC) and protein phosphatase 2a (PP2a) at the end of the pneumoperitoneum. After 1 week, the concentration of cancer cells in the culture medium was calculated. Result: When the pressure of CO 2 pneumoperitoneum was 0, 10, 20, 30 mmHg respectively, the average pHi was 7.273, 7.075, 6.783, 6.693 at the end of the pneumoperitoneum; PKC activity was 159.4, 168.5, 178.0, 181.6 nmol/(g⋅min) and PP2a was 4158.3, 4066.9, 3984.0, 3878.5 nmol/(g⋅min) respectively. After 1 week, the cancer cells concentration was 2.

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“…In in-vitro experiments, the SKOV3 ovarian carcinoma cell line was found to show increased growth under the influence of CO 2 [8]. By contrast, in various studies in which the nude rat model was used, neither the laparoscopy gas used nor laparoscopy itself was found to have any influence on various tumor parameters such as the weight of the omentum, metastases, survival of the animals or circulating tumor DNA, in comparison with laparotomy [5,9,10,11,12]. However, Canis et al [6] observed increased tumor growth after laparotomy in the BD IX rat model in comparison with animals that underwent laparoscopic procedures.…”

Section: Discussionmentioning

confidence: 92%

Influence of surgical trauma on tumor establishment in a rat ovarian cancer model

et al. 2004

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There is a strong debate regarding the influence of laparoscopic surgery on tumor dissemination and prognosis, especially in ovarian cancer. In murine mammary cancer cell line models, the incidence of surgical metastasis was influenced by the type of surgical trauma. We investigated the influence of surgical trauma on tumor establishment in a synergetic rat ovarian cancer model in 6-to 8-week-old female BD-IX rats using the rat ovarian cancer cell line O-342. The animals underwent i.p. injection of tumor cells, laparoscopy or midline laparotomy with different surgical procedures at the right uterine horn (grasping with a forceps, incision of the uterine horn after coagulation or partial resection of the uterine horn by ligation). Intraperitoneal tumor establishment was then determined 14 days after the surgical procedure, using cancer index scoring (0, no tumor; 1, tumor diameter less than 0.5 cm; 2, between 0.5 and <1.0 cm; 3, between 1.0 and <2.0 cm). No macroscopically visible tumor growth was observed in the uterine horn, which was unaffected by any surgical procedure. Alternatively, for the right uterine horn that underwent grasping by forceps, coagulation or ligation, the cancer index score was found to be 0.2, 2.5 and 3.0, respectively. The present study shows that surgical trauma favors the establishment of tumor lesions in the rat ovarian cancer model.

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Impact of pneumoperitoneum on tumor growth

Abstract: In these solid tumor models, CO2 pneumoperitoneum had no deleterious effect on tumor growth when compared to gasless laparoscopy or midline laparotomy.

Cited by 29 publications

References 23 publications

Higher CO₂-insufflation pressure inhibits the expression of adhesion molecules and the invasion potential of colon cancer cells

Higher CO₂-insufflation pressure inhibits the expression of adhesion molecules and the invasion potential of colon cancer cells

Influence of CO₂pneumoperitoneum on intracellular pH and signal transduction in cancer cells

Influence of surgical trauma on tumor establishment in a rat ovarian cancer model

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Impact of pneumoperitoneum on tumor growth

Abstract: In these solid tumor models, CO2 pneumoperitoneum had no deleterious effect on tumor growth when compared to gasless laparoscopy or midline laparotomy.

Cited by 29 publications

References 23 publications

Higher CO2-insufflation pressure inhibits the expression of adhesion molecules and the invasion potential of colon cancer cells

Higher CO2-insufflation pressure inhibits the expression of adhesion molecules and the invasion potential of colon cancer cells

Influence of CO2pneumoperitoneum on intracellular pH and signal transduction in cancer cells

Influence of surgical trauma on tumor establishment in a rat ovarian cancer model

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Higher CO₂-insufflation pressure inhibits the expression of adhesion molecules and the invasion potential of colon cancer cells

Higher CO₂-insufflation pressure inhibits the expression of adhesion molecules and the invasion potential of colon cancer cells

Influence of CO₂pneumoperitoneum on intracellular pH and signal transduction in cancer cells