Influence of CO₂pneumoperitoneum on intracellular pH and signal transduction in cancer cells

Abstract

Abstract:Object: The authors studied the influence of CO 2 pneumoperitoneum on intracellular pH and signal transduction arising from cancer cell multiplication in laparoscopic tumor operation. Method: They set up a simulation of pneumoperitoneum under different CO 2 pressure, and then measured the variation of intracellular pH (pHi) at different time and the activity of protein kinase C (PKC) and protein phosphatase 2a (PP2a) at the end of the pneumoperitoneum. After 1 week, the concentration of cancer cells in the culture medium was calculated. Result: When the pressure of CO 2 pneumoperitoneum was 0, 10, 20, 30 mmHg respectively, the average pHi was 7.273, 7.075, 6.783, 6.693 at the end of the pneumoperitoneum; PKC activity was 159.4, 168.5, 178.0, 181.6 nmol/(g⋅min) and PP2a was 4158.3, 4066.9, 3984.0, 3878.5 nmol/(g⋅min) respectively. After 1 week, the cancer cells concentration was 2.

In vitro evaluation of a simulated pneumoperitoneum environment using carbon dioxide on canine transitional cell carcinoma

“…However, there were no dose‐response effects of CO 2 exposure on cancer cell growth in our study. Similarly to our work, results of several studies have provided no evidence of significant effects of CO 2 exposure on the proliferation of carcinoma cells, whereas others have shown variable effects, depending on the cell line . It has also been previously reported that lower insufflation pressures (0 and 5 mmHg) result in no significant effects in proliferation or viability compared with control …”

“…This sequential change in viability and proliferation may be explained, at least in part, by the fact that, during the stress of the positive pressure CO 2 environment, some cells may undergo apoptosis, whereas others adapt to survive. Therefore, it is possible that only the cells with a more aggressive and resilient phenotype survived the positive pressure CO 2 environment . However, similar proliferation trends were observed in cells under standard conditions.…”