In vitro evaluation of a simulated pneumoperitoneum environment using carbon dioxide on canine transitional cell carcinoma

Asthana, Rashi; Oblak, Michelle L.; Singh, Ameet; Mutsaers, Anthony J.

doi:10.1111/vsu.12778

Cited by 3 publications

(2 citation statements)

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“…Experiments in vitro showed a nonlinear impact of CO 2 insufflation pressures on the viability and proliferation of tumor cell lines. 29,30 Asthana demonstrated a "U-shaped", dose-response effect of pneumoperitoneum pressure on the viability of transitional carcinoma cells and Madin-Darby canine kidney cells. 29 They described decreased viability in all cell lines upon exposure to 5 mmHg and 10 mmHg compared with that obtained with control and 0 mmHg, whereas no effects were observed in cells exposed to 15 mmHg of CO 2 pneumoperitoneum.…”

Section: Carbon Dioxide (Co 2 ) Pneumoperitoneummentioning

confidence: 99%

“…29,30 Asthana demonstrated a "U-shaped", dose-response effect of pneumoperitoneum pressure on the viability of transitional carcinoma cells and Madin-Darby canine kidney cells. 29 They described decreased viability in all cell lines upon exposure to 5 mmHg and 10 mmHg compared with that obtained with control and 0 mmHg, whereas no effects were observed in cells exposed to 15 mmHg of CO 2 pneumoperitoneum. Zheng supplied additional information on the effect of CO 2 pneumoperitoneum on the proliferation and migration of cells using a gastrointestinal stromal tumor line (GIST-T1).…”

Section: Carbon Dioxide (Co 2 ) Pneumoperitoneummentioning

confidence: 99%

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<p>The Pathogenesis and Prevention of Port-Site Metastasis in Gynecologic Oncology</p>

Gao¹,

Guo²,

Wang³

2020

CMAR

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Port-site metastasis (PSM) is a specific and challenging complication of laparoscopic gynecologic oncologic procedures. Research has demonstrated that PSM is associated with significant morbidity and poor outcome. The exact pathogenesis of PSM in gynecologic patients is not clear. Several preventive measures of PSM have been addressed in the relevant literature, and novel approaches to prevent this uncommon complication keep emerging. In this review, we summarized the potential mechanisms of PSM and discuss the controversies and merits of proposed preventive measures of PSM in gynecologic oncology. We undertook a literature search using the Medline database to identify studies on the pathogenesis and prevention of laparoscopic PSM. Hypotheses of PSM pathogeneses center on the immune response, pneumoperitoneum, wound contamination, and surgical method. Cogent evidence of effective prevention of PSM after laparoscopic surgery is lacking. Traditional preventive actions such as irrigation and tumor manipulation should be taken individually. Insufflation of hyperthermic CO 2 and humidified CO 2 leads to a better outcome in patients with a malignant tumor who undergo a laparoscopic procedure compared with normal CO 2 pneumoperitoneum. Port-site resection shows no advantage in survival and results in more wound events. PSM prevention plays a crucial part in the overall care of patients with gynecologic malignancies who undergo laparoscopic procedures.

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Section: Carbon Dioxide (Co 2 ) Pneumoperitoneummentioning

confidence: 99%

Section: Carbon Dioxide (Co 2 ) Pneumoperitoneummentioning

confidence: 99%

<p>The Pathogenesis and Prevention of Port-Site Metastasis in Gynecologic Oncology</p>

Gao¹,

Guo²,

Wang³

2020

CMAR

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Perioperative Detection of Circulating Tumor Cells in Radical or Partial Nephrectomy for Renal Cell Carcinoma

et al. 2019

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Proteomic analysis of the influence of CO2 pneumoperitoneum in cervical cancer cells

Zhou

2021

Journal of Cancer Research and Therapeutics

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Objective: The effect of CO2 pneumoperitoneum (CDP) on the oncology outcomes of patients undergoing laparoscopic radical hysterectomy for cervical cancer remains unclear. In this study, we investigated the effects of CDP on the proliferation of cervical cancer cells and examined the molecular mechanism. Materials and Methods: We established an in vitro CDP model to study the effects of CDP on the proliferation of cervical cancer cells by Cell Counting Kit-8 (CCK-8) assay, xenografted tumor assay. Tandem mass tag-based quantitative proteomics were used to study the proteomic changes in HeLa cells after CDP treatment. Western blot assay was used to detect the expressions of PI3K/Akt signaling pathway proteins. Results: CDP increased cell proliferation after a short period of inhibition in vitro and promoted tumorigenesis in vivo. Proteomic analysis showed that the expression levels of 177 and 309 proteins were changed significantly 24 and 48 h after CDP treatment, respectively. The acidification caused by CO2 inhibited the proliferation of cervical cancer cells by inhibiting the phosphorylation of PI3K and Akt. Conclusions: CDP promoted the proliferation of human cervical cancer cells after a short time of inhibition. The mechanism of which is related to the inhibition of phosphorylation of the PI3K/Akt signaling pathway.

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In vitro evaluation of a simulated pneumoperitoneum environment using carbon dioxide on canine transitional cell carcinoma

Cited by 3 publications

References 46 publications

<p>The Pathogenesis and Prevention of Port-Site Metastasis in Gynecologic Oncology</p>

<p>The Pathogenesis and Prevention of Port-Site Metastasis in Gynecologic Oncology</p>

Perioperative Detection of Circulating Tumor Cells in Radical or Partial Nephrectomy for Renal Cell Carcinoma

Proteomic analysis of the influence of CO2 pneumoperitoneum in cervical cancer cells

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